Novel watermelon sugar transporter as well as coding gene ClVST1 and application thereof

A technology of transgenic plants and proteins, applied in application, genetic engineering, plant genetic improvement, etc., can solve the problems of low selection efficiency, high cost, and heavy workload.
CN114656533AActive Publication Date: 2022-06-24BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
Publication Date
2022-06-24

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Abstract

The invention belongs to the field of gene engineering, and relates to a novel watermelon sugar transporter and a coding gene ClVST1 and application thereof. The novel watermelon sugar transport protein has a sequence composition shown in a sequence 1 in a sequence table, and the coding gene ClVST1 has a sequence composition shown in a sequence 2 in the sequence table. The invention also relates to application of the novel watermelon sugar transporter protein and the novel watermelon sugar transporter coding nucleic acid sequence in watermelon disease and insect pest resistance. The invention further provides an obtaining method of the watermelon plant disease and insect pest resistant strain. The watermelon novel sugar transporter and the coding gene ClVST1 provided by the invention are closely related to disease and insect pest resistance, and the higher the expression quantity is, the stronger the disease and insect pest resistance is; therefore, a stable watermelon strain resistant to bacterial fruit blotch and aphid can be obtained by knocking out novel sugar transport of watermelons in watermelon plants, and a quick and efficient way is provided for breeding of watermelons resistant to diseases and insect pests.
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Description

technical field

[0001] The invention belongs to the field of biotechnology, and particularly relates to a novel watermelon sugar transporter and its encoding gene CLVST1 and application. Background technique

[0002] The sugar produced by photosynthesis in watermelon leaves is transported to the fruit through the stem phloem. In the fruit, it needs to go through four steps of metabolism, unloading, transport and storage, that is, oligosaccharides such as raffinose are hydrolyzed into sucrose, the phloem of the fruit unloads the sucrose, and the sugar in the intercellular space is composed of Sugar transport proteins are transported into pulp cells and ultimately store sugar in pulp cell vacuoles. The novel sugar transporter CLVST1 is a key factor regulating fruit sugar unloading, that is, a key gene that plays a role in the second link unloading. "

[0003] Watermelon (Citullus lanatus (Thunb.) Matsum & Nadai) is an important horticultural crop in the world. my country's w...

Claims

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