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Application of chitosan in prevention and control of bacterial fruit blotch of plants

A chitosan and bacterial technology is applied to the application field of chitosan in the prevention and control of plant bacterial fruit spot, which can solve the problems of poor control effect and few types of chemicals, and achieves convenient operation, easy popularization and application, prevention and control. good effect

Inactive Publication Date: 2012-08-01
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The invention provides the application of chitosan in the prevention and treatment of plant bacterial fruit spot, which has the advantages of simple process, good safety and good control effect, and solves the problems of few types of medicaments and poor control effect, etc.

Method used

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  • Application of chitosan in prevention and control of bacterial fruit blotch of plants
  • Application of chitosan in prevention and control of bacterial fruit blotch of plants
  • Application of chitosan in prevention and control of bacterial fruit blotch of plants

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Embodiment 1 prepares chitosan solution

[0029] Get the chitosan powder that deacetylation degree is 85% and be dissolved in the acetic acid aqueous solution of 1% by volume percentage, be mixed with the solution that chitosan concentration is 5mg / ml; Regulate pH to 6.0 with NaOH, shake with 160rpm at room temperature 24h; sterilize in an autoclave at 121°C for 20min, and set aside.

Embodiment 2

[0030] Embodiment 2 prepares watermelon fruit spot fungus PFB-01 seed solution

[0031] Watermelon fruit spot fungus PFB-01 was isolated from watermelon samples infected in Zhejiang Province and stored in the Fungal Collection Center of Institute of Biotechnology, Zhejiang University. Streak the watermelon fruit spot fungus PFB-01 on the NA agar plate and culture it at 28°C for 48 hours; pick a single colony and inoculate it into the LB liquid medium, and culture it in a shaker at 28°C and 160r / min 24h, make seed liquid, make the bacterium concentration in the seed liquid be 10 9 CFU / ml.

Embodiment 3

[0032] The effect of embodiment 3 chitosan concentration on the growth of watermelon fruit spot fungus PFB-01

[0033] The 5mg / ml chitosan solution of embodiment 1 is diluted into the chitosan solution that concentration is 0.05,0.10,0.20,0.40mg / ml; The watermelon fruit blotch PFB-01 seed liquid in the inoculation embodiment 2, makes Bacterial concentration is 10 8 CFU / ml. After cultured on a shaking table at 28°C and 160rpm for 6 hours, the number of viable bacteria of P. PFB-01 was determined by plate counting. Sterile deionized water with a pH of 6.0 was used as the control of chitosan treatment in the experiment.

[0034] The result is as figure 1As shown, different concentrations of chitosan solutions have significant inhibitory effect on watermelon fruit spot fungus PFB-01, and the inhibitory effect increases with the increase of chitosan concentration. After treatment with 0.05mg / ml chitosan solution, the number of viable bacteria of watermelon fruit spot fungus PFB...

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Abstract

The invention discloses application of chitosan in prevention and control of bacterial fruit blotch of plants, wherein the deacetylation degree of the chitosan is more than 85%. The plants are melon plants. The application comprises the steps of placing plant seeds in a chitosan solution to be soaked for 3-5 hours or using the chitosan solution to perform spraying treatment to leaves of the plants at a trefoil stage. The chitosan uses as a main raw material, the prepared and obtained chitosan solution can effectively inhibit the activity of the bacterial fruit blotch and prevent and control the bacterial fruit blotch of the plants. The chitosan solution with certain concentration further can effectively promote growth of watermelon seedlings. The raw material is easily obtained, the process is simple, the operation is convenient, and the chitosan is low in toxicity and harm and good in preventing and controlling effect on the bacterial fruit blotch of melon plants. In addition, the chitosan can further promote the growth of plant seedlings to some extent and is suitable for actual production and easy to popularize and apply.

Description

technical field [0001] The invention relates to the technical field of plant protection biological control, in particular to the application of chitosan in the control of plant bacterial fruit spot. Background technique [0002] Melon has always been a very important vegetable variety, especially watermelon, which is juicy, sweet, cool and delicious, and has become an indispensable summer commodity in the daily life of residents. Farmers have also gained better economic benefits from planting watermelons. However, in recent years, after watermelon bacterial spot disease infects watermelon, water-soaked dots appear on the surface of the fruit, which spread and penetrate into the pulp, causing the watermelon fruit to rot and lose its edible value, causing huge economic losses to melon farmers. [0003] Bacterial fruit spot was first discovered in Florida, USA, and Crall et al. described its symptoms, but did not identify the pathogenic bacteria (Crall et al.1969). In 1988, W...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N43/16A01P1/00A01P21/00A01C1/00A01G13/00
Inventor 李斌石雨周青单长林谢关林
Owner ZHEJIANG UNIV
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