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Cucurbit bacterial fruit blotch biocontrol bacillus amyloliquefaciens strain and application thereof

A technology of Bacillus amyloliquefaciens and Bacillus amyloliquefaciens, which is applied in the field of Bacillus amyloliquefaciens strains for the biocontrol of bacterial fruit spot disease of melons, and can solve the problem of biocontrol strains of Bacillus amyloliquefaciens that have not been seen in melons and affect seeds. Germination, easy to produce phytotoxicity and other problems, to achieve the effect of alleviating antibiotic pollution and phytotoxicity, neat seedling emergence, and good practical application value

Active Publication Date: 2015-09-09
INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Fruit spot disease is a disease transmitted by seeds. At present, hydrochloric acid, formalin and other chemical agents are mainly used to treat seeds, which are prone to phytotoxicity and affect seed germination.
So far, there has been no report on the use of Bacillus amyloliquefaciens to treat seeds to control bacterial fruit spot of melons

Method used

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  • Cucurbit bacterial fruit blotch biocontrol bacillus amyloliquefaciens strain and application thereof
  • Cucurbit bacterial fruit blotch biocontrol bacillus amyloliquefaciens strain and application thereof
  • Cucurbit bacterial fruit blotch biocontrol bacillus amyloliquefaciens strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] 1.1 Identification of antagonistic bacteria JD001 for the control of melon bacterial fruit spot

[0047] 1.1.1 Screening of antagonistic bacteria

[0048] Pick a single colony of Pseudomonas spp. and inoculate it in liquid LB medium (Tryptone (Tryptone) 10g / L, yeast extract (Yeast extract) 5g / L, sodium chloride (NaCl) 5g / L), 30°C, 200r / L min constant temperature culture for 24h, OD600≈0.3(10 8 cfu / mL). Add the bacterial liquid to the medium (the ratio of the bacterial liquid to the medium is 1:40) to make a mixed bacterial plate. Use a sterilized toothpick to pick up antagonistic bacteria colonies and spot them on the mixed bacteria plate. Incubate at a constant temperature of 30°C, observe the antibacterial effect at 24h and 49h, and measure the size and presence of the antibacterial zone. From the antibacterial effect of the plate, the indoor plate antibacterial effect of the JD001 strain is good, the average diameter of the inhibition zone is 24.5mm, and the anta...

Embodiment 2

[0058] Example 2 Antagonist JD001 seed treatment greenhouse potted plant disease control experiment and indoor growth promotion effect.

[0059] Adjust the Aac-5 bacterial solution to 10 8 cfu / mL, mixed with 1g of melon seeds and 2mL of bacterial solution, soaked for 1h, and dried the seeds in an ultra-clean workbench.

[0060] Melon seeds inoculated with fruit blotch fungus in JD001 bacterial solution (10 8 cfu / mL) for 12 hours, 50 seeds per dish, repeated 3 times. Accelerate the germination at 28°C, investigate the germination rate of the seeds after 24 hours, and measure the root length after 48 hours, so as to judge the influence of the chemical treatment on the germination of the seeds. The remaining seeds of the same treatment were sown in sterilized soil, and each treatment was repeated 3 times. Clear water was used as a control. After one week of investigation after emergence, the melon seeds treated with Bacillus amyloliquefaciens bacteria solution did not suffe...

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Abstract

The invention provides a cucurbit bacterial fruit blotch biocontrol bacillus amyloliquefaciens strain and the application thereof. The biological preservation number of the obtained cucurbit bacterial fruit blotch biocontrol bacillus amyloliquefaciens strain JD001 is CGMCC No.10421. The strain JD001 is separated from a soil sample of a watermelon producing area and is preserved, has good bacteriostasis on a plurality of kinds of phytopathogen bacteria and can be used for biological control over cucurbit bacterial fruit blotches. New microbial resources are provided for prevention and cure of the cucurbit bacterial fruit blotches, and the good application prospect is achieved.

Description

technical field [0001] The invention belongs to biotechnology technology, and relates to a bacterial strain of bacillus amyloliquefaciens for the control of melon bacterial fruit spot disease and its application. Background technique [0002] Cucurbit bacterial fruit spot is a typical seed-borne bacterial disease, and the pathogen is Acidovorax citrulli. The fungus can infect a variety of cucurbit crops, such as melon, watermelon, melon, pumpkin, cucumber, etc. Artificial inoculation can also infect plants such as betel leaves. The disease was first discovered in the mid-1960s, when it was considered a seedling wilt by the United States Department of Agriculture (USDA), and it soon developed into an important disease in the melon producing areas of the United States. In 1989, the disease occurred in large numbers in commercial seed production bases in the United States, causing devastating damage to the American melon industry. The disease affects all stages of watermelons...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01P1/00A01C1/00A01G7/06C12R1/07
Inventor 赵廷昌孙柏欣杨玉文关巍王铁霖董春玲
Owner INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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