Colloidal gold nucleic acid test strip for watermelon bacterial fruit blotches as well as preparation and application thereof

Abstract

The invention discloses a colloidal gold nucleic acid test strip for watermelon bacterial fruit blotches as well as preparation and application thereof, which belong to the technical field of colloidal gold detection. A detected substance is firstly bound with colloidal gold coupled Aptamers (1) in a competitive way under the action of a capillary siphoning effect formed by an absorbent pad, and residual aptamers drift to a detection zone to be bound with streptavidin-Aptamers (2) and realize color development in the presence of excessive coupled Aptamers (1); and the V region binding sites of the colloidal gold coupled Aptamers (1) bound with the detecting substance are occupied by the detected substance, so that the Aptamers can only cross the detection zone and drift to a reference zone to be unspecifically bound with streptavidin-Aptamers (3) at binding sites in a C region and carry out color comparison with the detection zone to semi-quantitatively detect the residual quantity of watermelon bacterial fruit blotches in the sample. The test strip can meet the requirement of food safety on the residual quantity of watermelon bacterial fruit blotches, is suitable for feeds, meat producing plants and government detection mechanisms, and has the characteristics of convenience for using, economical efficiency, rapidness, easiness in manufacturing and low cost.

Description

technical field [0001] The invention discloses a method for detecting watermelon fruit spot virus with a gold-labeled test strip of a colloidal gold-labeled aptamer, which belongs to the technical field of colloidal gold detection. Background technique [0002] Watermelon and melon diseases are common, and some have been effectively controlled. However, bacterial fruit rot of melons, also known as Bacterial Fruit Blotch (BFB for short), is a disease that has been on the rise in recent years and poses a great threat to the production of melons. After the disease occurs in the fruit, drug control is basically ineffective, the fruit rots and loses its commodity value, and the melon farmers suffer heavy losses. Since the main mode of transmission of the disease is seed infection, it also has a serious impact on my country's watermelon and melon breeding industry and seed export. [0003] The occurrence of bacterial fruit rot has become one of the urgent problems to be solved i...

AI Technical Summary

Problems solved by technology

At present, a lot of research has been done on the identification of pathogenic bacteria, detection technology, and treatment of infected seeds at home and abroad, but the basic research on the infection cycle is still very weak.

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