Compound microbial fertilizer for antagonism of soil-borne fungal diseases, and preparation method and application thereof

A technology of compound microorganisms and soil-borne fungi is applied in the field of compound microbial fertilizers that antagonize soil-borne fungal diseases and its preparation, which can solve the problems of inability to produce antagonism, and achieve strong in-situ enhancement effect and biomimetic strain effect, good effect, Good disease prevention effect

Active Publication Date: 2015-09-09
QINGDAO GENYUAN BIOLOGICAL TECH GRP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Then, microbial compound fertilizer products mostly use a combination of two or more bacteria, aiming to synthesize the effects of multiple bacteria and complement each other, but the premise of the combination of bacteria and bacteria is that they cannot produce antagonistic effects

Method used

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  • Compound microbial fertilizer for antagonism of soil-borne fungal diseases, and preparation method and application thereof
  • Compound microbial fertilizer for antagonism of soil-borne fungal diseases, and preparation method and application thereof
  • Compound microbial fertilizer for antagonism of soil-borne fungal diseases, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1. Isolation, screening, identification and preservation of Bacillus amyloliquefaciens HFJ-7

[0035] 1. Isolation and screening of Bacillus amyloliquefaciens HFJ-7

[0036] From January 2012 to March 2012, 30 pepper rhizosphere soil samples were taken from 10 pepper vegetable greenhouses in Shangkou Town, Shouguang City, Weifang City, Shandong Province. The soil samples were fully ground respectively, heated in a water bath at 75°C for 20 minutes, then diluted 1000 times with sterile saline, diluted and spread on a nutrient agar NA plate, and single colonies were picked and purified by streaking, and a total of 68 strains of pure Bacteria, all of which were Bacillus after microscopic examination, including strain HFJ-7.

[0037] 2. Comparison of pathogenic fungal resistance between Bacillus amyloliquefaciens HFJ-7 and other strains

[0038] Using the method of plate confrontation, the pathogenic fungi of soil-borne diseases such as Rhizoctonia solani, Fusar...

Embodiment 2

[0045] Example 2, Bacillus amyloliquefaciens HFJ-7 has the characteristics of antagonizing various pathogenic fungi

[0046] Using the plate confrontation method, the antagonistic characteristics of different Bacillus were investigated by using Rhizoctonia solani, Fusarium oxysporum, root rot pathogen, cucumber wilt pathogen, Capsicum phytophthora and other soil-borne disease pathogenic fungi as target pathogenic fungi difference. First use a puncher to obtain a 6mm-diameter pathogenic bacteria block, connect it to the center of the PDA plate, and inoculate the target bacteria line with a length of about 1cm around the plate, vertically about 3cm from the center. Considering the different growth rates of different pathogenic fungi, Rhizoctonia solani was pre-cultured for 8 hours, Fusarium oxysporum, root rot pathogen, cucumber wilt pathogen, and Capsicum phytophthora were pre-cultured for 24 hours, and then inoculated with antagonistic bacteria, including amyloliquefaciens ...

Embodiment 3

[0048] Example 3, Bacillus amyloliquefaciens HFJ-7 has stronger liquid fermentation proliferation ability

[0049] Pick single colonies of Bacillus subtilis G4, Bacillus lateralis M9, Bacillus amyloliquefaciens HFJ-7 and HFJ-8 respectively, and inoculate them in test tubes containing 5ml sterilized LB liquid medium, at 37°C, 160 rpm / min , shaking and culturing for 12 hours; at a ratio of 1%, transfer to a 500ml Erlenmeyer flask containing 130ml LB liquid medium, 37°C, 180 rpm / min shaking and culturing for 18 hours, using Hengping 722 visible spectrophotometer, using colorimetric method, Track and monitor OD 660nm; sampling time is 0, 2, 4, 6, 8, 10, 12, 15, 18h. Each experimental group had 3 repetitions at each time point.

[0050] figure 2 Show and understand the comparative growth curves of Bacillus amylogenus HFJ-7 and other three strains of Bacillus. The results showed that in the LB medium, the order of the growth and reproduction ability of several bacillus from st...

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Abstract

The invention discloses a compound microbial fertilizer for antagonism of soil-borne fungal diseases, and a preparation method and an application thereof; the fertilizer takes humic acid as a compound carrier, includes bacillus amyloliquefaciens HFJ-7 with the bacteria content of 1.8*10<8>-2.1*10<8> CFU/g, and also includes bacillus megatherium B3. The bacillus amyloliquefaciens HFJ-7 in the compound microbial fertilizer has the preservation number of CGMCC No.10011. The bacillus amyloliquefaciens HFJ-7 strain can be antagonistic to rhizoctonia solani, fusarium oxysporum, root rot pathogen, cucumber fusarium wilt pathogen, phytophthora capsici leon pathogen and other soil-borne disease pathogenic fungi. After the bacillus amyloliquefaciens HFJ-7 is compounded with the bacillus megatherium B3, the resistance to rhizoctonia solani and pepper root rot pathogen can be significantly improved, a disease state index of pepper seedling root rot disease can be significantly reduced, and the occurrence and spreading of pepper root rot can be effectively prevented and controlled.

Description

technical field [0001] The invention belongs to the field of biological fertilizers and relates to the preparation and application of compound microbial fertilizers, in particular to a compound microbial fertilizer for antagonizing soil-borne fungal diseases and its preparation method and application. Background technique [0002] In recent years, great progress has been made in the control of plant diseases by bacteria. According to reports in the United States, the effect of using Erwinia herbophilus to control amylovora is equivalent to that of streptomycin. The Biological Pesticide Engineering Center of Shenyang Agricultural University used antagonistic Trichoderma and antagonistic bacteria to make a powder, which successfully prevented the seedling diseases of protected vegetables and melons. The product is in the pilot stage. Other reported bacterial fungicides include: Bacillus licheniformis used to control cucumber and tobacco anthracnose, Bacillus subtilis to contr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01P3/00C12R1/11C12R1/07
CPCA01N63/00C05F11/02C05F11/08C05G3/80C12N1/20
Inventor 李俊安李慧芬王娇马成薛玉赵志强张明俊王晓伟
Owner QINGDAO GENYUAN BIOLOGICAL TECH GRP
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