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High-yield strain streptomyces lydicus, breeding and fermentation thereof

A technology of Streptomyces lidi and high-yielding strains, applied in the directions of fermentation, bacteria, fungicides, etc., can solve the problems of low production capacity of starting strains and inability to meet the requirements of actual production.

Active Publication Date: 2010-04-14
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] A Streptomyces lydicus strain (Streptomyces lydicus AS 4.2501) CGMCC NO.1692 was isolated from the soil in my laboratory. Its fermentation broth has a broad-spectrum antifungal effect, but the production capacity of the starting strain is too low to meet the actual production requirements. requirements

Method used

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  • High-yield strain streptomyces lydicus, breeding and fermentation thereof
  • High-yield strain streptomyces lydicus, breeding and fermentation thereof
  • High-yield strain streptomyces lydicus, breeding and fermentation thereof

Examples

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Effect test

Embodiment 1

[0071] The breeding of high-yielding bacterial strain Streptomyces lydides E9 consists of the following steps:

[0072] (1) Natural breeding of the starting strain: at room temperature, the concentration of 0.1mL was 10 7 Streptomyces lydicus AS 4.2501 (Streptomyces lydicus AS 4.2501) CGMCC NO.1692 single spore suspension per mL was added to the separation medium plate, and then spread evenly with a spreader, and cultivated at 30°C until needle-tip-sized spores grew on the plate. For white colonies, use a puncher with an inner diameter of 8 mm to punch holes, pick a single colony agar block and culture it in a shake flask, then filter the shake flask fermentation broth of each strain and dilute it 10 times, then use the double-layer plate cup and saucer method Measure the antibacterial activity, and select the strain with good antibacterial effect as the starting strain for the next stage;

[0073] (2) Compound mutagenesis and self-product resistance screening: at room temper...

Embodiment 2

[0075] The breeding of high-yield bacterial strain Streptomyces lydida E9 consists of the following steps:

[0076] (1) Natural breeding of the starting strain: at room temperature, the concentration of 0.1mL was 10 2 Streptomyces lydicus AS 4.2501 (Streptomyces lydicus AS 4.2501) CGMCC NO.1692 single spore suspension per mL was added to the separation medium plate, then spread evenly with a spreader, and cultured at 26°C until pinpoint-sized pincushions grew on the plate. For white colonies, use a puncher with an inner diameter of 8 mm to punch holes, pick a single colony agar block and culture it in a shake flask, then filter the shake flask fermentation broth of each strain and dilute it 10 times, then use the double-layer plate cup and saucer method Determination of antibacterial activity, select the bacterial strain with good antibacterial effect as the starting strain of the next stage; (2) compound mutagenesis and self-product resistance screening: at room temperature, ...

Embodiment 3

[0078] The breeding of high-yield bacterial strain Streptomyces lydida E9 consists of the following steps:

[0079] (1) Natural breeding of the starting strain: at room temperature, the concentration of 0.1mL was 10 8 Streptomyces lydicus AS 4.2501 (Streptomyces lydicus AS 4.2501) CGMCC NO.1692 single spore suspension per mL was added to the separation medium plate, then spread evenly with a spreader stick, and cultured at 28°C until needle-tip-sized spores grew on the plate. For white colonies, use a puncher with an inner diameter of 8 mm to punch holes, pick a single colony agar block and culture it in a shake flask, then filter the shake flask fermentation broth of each strain and dilute it 10 times, then use the double-layer plate cup and saucer method Measure the antibacterial activity, and select the strain with good antibacterial effect as the starting strain for the next stage;

[0080] (2) Compound mutagenesis and self-product resistance screening: at room temperatur...

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Abstract

The invention discloses a high-yield strain streptomyces lydicus, as well as breeding and fermentation thereof. The fermentation of the high-wield stain streptomyces lydicus E9 CGMCC No.3075 comprises the following steps: (1) preparation of a shake flask, seeds and a fermentation medium; (2) shake flask culture; (2) seed culture; (4) fermentation pot culture: leading a seed culture liquid in a fermentation pot filled with the fermentation medium and culturing for 24-96 hours to obtain a high-wield stain streptomyces lydicus E9 fermentation liquor under the condition of ventilating, stirring, temperature of 25-35 DEG C, and pH of 4-9; and carrying out ultrasonication for 20-40 min. The strain of the invention can generate large quantity of substances with antimycotic activity in the fermentation liquor, and the fermentation liquor has evident inhibiting effect on rhizoctonia solani; and the bacterial inhibition rate of the fermentation liquor which is diluted by 50 times reaches above 97.8% after 48 hour fermentation.

Description

technical field [0001] The invention belongs to the field of industrial microbes, and relates to a new high-yield strain of Streptomyces lydides and the breeding and fermentation of the strain. Background technique [0002] In my country, plant fungal diseases rank first among the three major plant diseases (bacteria, fungi, and viruses). If they are not prevented and controlled in time, they will cause huge losses to agriculture and forestry. At present, chemical pesticides are mostly used to prevent and control plant fungal diseases. Although chemical pesticides have quick effects, their action time is short, causing serious environmental pollution, and pathogenic bacteria are prone to develop resistance to chemical pesticides, which will eventually be replaced by other efficient and environmentally friendly drugs. substitute. Microbial pesticides are highly efficient and environmentally friendly pesticides. [0003] A Streptomyces lydicus strain (Streptomyces lydicus AS...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N15/01C12P1/04A01P3/00C12R1/465
Inventor 元英进程景胜韦宇平陈伟
Owner TIANJIN UNIV
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