Preparation method and applications of wild jujube endophytic bacillus amyloliquefaciens new strain SZ23 and fermentation broth

A technology of amylolytic spores and new strains, which is applied in the field of utilization of plant endophytic bacteria, can solve the problems of being easily degraded, and achieve the effect of not harsh fermentation conditions and simple preparation process

Inactive Publication Date: 2015-04-08
SHANXI AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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  • Preparation method and applications of wild jujube endophytic bacillus amyloliquefaciens new strain SZ23 and fermentation broth
  • Preparation method and applications of wild jujube endophytic bacillus amyloliquefaciens new strain SZ23 and fermentation broth
  • Preparation method and applications of wild jujube endophytic bacillus amyloliquefaciens new strain SZ23 and fermentation broth

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Embodiment 1

[0056] A strain of Bacillus amyloliquefaciens ( Bacillus amyloliquefaciens ) The new strain SZ23 was deposited in the General Microbiology Center of the China Committee for the Collection of Microbial Cultures on July 25, 2014. The preservation number is CGMCC No. 9481. Address: No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing, Chinese Academy of Sciences Microbiology graduate School.

[0057] Bacillus amyloliquefaciens of the present invention ( Bacillus amyloliquefaciens ) The new strain SZ23 is an endophytic bacterium isolated from the root tissue of jujube plants in Taigu area, Shanxi Province.

[0058] The new strain SZ23 of Bacillus amyloliquefaciens sour jujube was cultured on NA medium at a constant temperature of 30°C. The initial colonies were milky white, round, pus-like, with neat edges, raised colonies, and sticky surface; with the extension of culture time, the colonies Dried, wrinkled and changed color to pale yellow ( figure 1 ). Observati...

Embodiment 2

[0065] The new strain SZ23 fermentation liquid of Bacillus amyloliquefaciens of the present invention is used to control pear black spot.

[0066] 1, the preparation method of bacillus amyloliquefaciens new bacterial strain SZ23 fermented liquid is as follows:

[0067] (1) Preparation of seed solution Pick the strain SZ23 from the slant of the test tube with an inoculation needle onto the slant medium, incubate at a constant temperature of 25°C for 24 hours, inoculate it into a conical flask containing 100 mL of LB culture solution, and culture it with shaking at 180 r / min for 12 hours. Prepare the seed solution of the new strain SZ23 of Bacillus amyloliquefaciens;

[0068] (2) Fermentation broth preparation The seed liquid of the new strain SZ23 of Bacillus amyloliquefaciens was inserted into 20 parts by mass and volume of soluble starch, 5 parts of yeast extract, 10 parts of peptone, K 2 HPO 4 ·3H 2 In the fermentation medium made of 2 parts of O and 1000 parts of distill...

Embodiment 3

[0071] The new strain SZ23 fermentation liquid of Bacillus amyloliquefaciens of the present invention is used to control pear black spot.

[0072] 1, the preparation method of bacillus amyloliquefaciens new bacterial strain SZ23 fermented liquid is the same as embodiment 2.

[0073] 2. Dilute the fermentation liquid of the new strain of Bacillus amyloliquefaciens SZ23 50 times with water, soak the pears in the diluted solution for 2 min to 3 min immediately after picking, and put them in plastic turnover boxes. Chill and store. Note that during the natural drying process, avoid contacting the pome fruit with soil and other substances that may carry pathogenic bacteria. This method can also effectively prevent the infection of pear fruit field pathogens during the postharvest storage period, and reduce the occurrence of postharvest black spot of pears during the storage period. According to the sampling survey, the control effect of postharvest black spot on pears has reached...

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Abstract

The invention discloses a preparation method and applications of wild jujube endophytic bacillus amyloliquefaciens new strain SZ23 and fermentation broth. The bacillus amyloliquefaciens new strain SZ23 is preserved at China General Microbiological Culture Collection Center on July, 25th, 2014, and preservation number is CGMCC No.9481. Morphological characteristics, physiological and biochemical indexes, and 16S rDNA sequencing results of bacillus amyloliquefaciens new strain SZ23 are detailedly disclosed by the invention. It is shown by bacteriostasis experiments that, the fermentation broth of bacillus amyloliquefaciens new strain SZ23 possesses excellent bacteriostatic activity on alternaria kikuchiana, fusarium oxyspirum F sp niveum, fusarium oxysporum Schl.f.sp vasinfectum(Atk.)Snyd.& Hans, alternaria solani, fusarium wilt of cucumber, cotton rhizoctonia solani kuhn, fusarium graminearum schw, and botrytis cirerea Pers et Tris; bacteriostasis rate ranges from 55.6% to 92.2%; and it is confirmed that wild jujube endophytic bacillus amyloliquefaciens new strain SZ23 possesses excellent broad-spectrum antibacterial activity. It is confirmed by practical experiments that control rate of the fermentation broth of new strain SZ23 on alternaria kikuchiana is more than 75%.

Description

technical field [0001] The present invention relates to the utilization field of plant endophytic bacteria, in particular to a kind of endophytic Bacillus amyloliquefaciens ( Bacillus amyloliquefaciens ) The preparation method and application of the new strain SZ23 and its fermentation broth. Background technique [0002] Plant endophytic bacteria (endophytic bacteria) are bacteria living in plant cells or intercellular spaces, which can establish a harmonious relationship with them. Endophytic bacteria in plants do not cause changes in phenotypic characteristics or functions of plants. After Paestur obtained endophytic bacteria from grapes in 1876, people really began to study endophytic bacteria in plants. In 1992, Kleopper first proposed the concept of "endophytic bacteria", which led botanists, microbiologists and microecologists to conduct a lot of research on it. Current studies have confirmed that endophytic bacteria exist in healthy plants and also have various bi...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/02A01P3/00C12R1/07
CPCA01N63/10C12N1/20C12N1/205C12R2001/07
Inventor 刘慧平刘东刘慧芹张宝俊韩巨才张晓宇任璐
Owner SHANXI AGRI UNIV
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