Bacillus velezensis and application thereof in preventing and treating clubroot of cruciferae

A technology of bacillus and strains, applied in the application, pest control, bacteria and other directions, can solve the problem of less bacillus velesi

Active Publication Date: 2021-06-04
INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are few researches and reports on the use of Bacillus Velez in biological control

Method used

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  • Bacillus velezensis and application thereof in preventing and treating clubroot of cruciferae
  • Bacillus velezensis and application thereof in preventing and treating clubroot of cruciferae
  • Bacillus velezensis and application thereof in preventing and treating clubroot of cruciferae

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1 10

[0081] Example 1 Isolation and screening of cruciferous clubroot biocontrol bacteria

[0082] 1. Isolation and purification of strains

[0083] Soil samples for testing: 128 rhizosphere soil samples were collected from areas where cruciferous vegetable clubroot occurred in Wulong, Chongqing, Enshi, Hubei, Sichuan, etc., marked with sterile sample bags and brought back to the laboratory for timely storage and processing.

[0084] 1198 strains of bacteria were isolated from the above 128 soil samples by dilution plate coating method. The specific operation is as follows: respectively add 10 g of soil samples into a Erlenmeyer flask filled with 90 mL of sterile distilled water, place the Erlenmeyer flask on a shaker at 28°C and 200 r / min for 30 minutes to fully suspend it, and treat it in a water bath at 80°C for 10 minutes to obtain 10 -1 The soil dilution solution, after gradient dilution to 10 -6 , will be 10 -4 、10 -5 and 10 -6 Draw 100 μL of each liquid and spread it on...

Embodiment 2

[0099] Identification of embodiment 2 bacterial strain ZF481

[0100] The bacterial strain ZF481 isolated and screened in Example 1 was taken for the following identification.

[0101] 1. Morphological characteristics

[0102] The morphological characteristics of the strain ZF481 were observed by the plate streak method. The shape of the bacteria was rod-shaped, and the average size of the bacteria was 0.4 μm×4.0 μm. The strain ZF481 was inoculated on the LB solid medium and cultured at a constant temperature of 28°C. box, and cultured upside down for 24 hours, and then observe the shape, color, edge uniformity, and texture of the single colony of the strain, as follows: the single colony of ZF481 is white, with a raised center, and the edges are neat and smooth. After growing in the medium for a period of time, the epidermis Folded, crater-shaped in the center of the colony.

[0103] 2. Physiological and biochemical characteristics

[0104] Refer to the "Common Bacteria Sy...

Embodiment 3

[0122] The control effect of embodiment 3 Veles bacillus ZF481 to clubroot

[0123] Pot test was adopted to detect the control effect of Bacillus Velez ZF481 on clubroot, and the method steps were as follows:

[0124] 1. Preparation and inoculation of dormant spore suspension of Plasmodium

[0125] Take out the preserved swollen roots of cabbage from the refrigerator at -20°C, wash and dry them after thawing, and put them in room temperature and dark conditions to rot for 5 days. Add an appropriate amount of distilled water to the tissue crusher to fully crush, filter the spore suspension through 8 layers of gauze, and adjust the concentration of dormant spores to a concentration of 1×10 using a hemocytometer. 8 pcs mL -1 , to obtain a suspension of dormant spores of Plasmodium.

[0126] The seeds of the Chinese cabbage variety 'Juxin' were soaked in warm water at 55 °C for 30 min, and washed twice with sterile water. The soaked cabbage seeds were neatly placed in a petri ...

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Abstract

The invention discloses bacillus velezensis ZF481 and application thereof. The strain number of the bacillus velezensis is ZF481, the bacillus velezensis is preserved in the China General Microbiological Culture Collection Center on July 8, 2020, and the registration number of the bacillus velezensis is CGMCC No.20320. The bacillus velezensis ZF481 or / and the metabolite of the bacillus velezensis ZF481 can be used for preventing and / or treating the plant clubroot, or preparing a product for preventing and / or treating the plant clubroot. The strain has an inhibiting effect on the activity and germination of plasmodiophora brassicae resting spores, has a good growth promoting effect on brassicaceous crops such as Chinese cabbage and the like, and also has a good antibacterial effect on phytophthora capsici, stemphylium solani, fusarium oxysporum, alternaria solani, corynespora, Botryis cinerea, rhizoctonia solani and the like.

Description

technical field [0001] The invention relates to a strain of Bacillus Velez ZF481 in the field of biotechnology and its application. Background technique [0002] Clubroot of cruciferous vegetables caused by Plasmodiophora brassicae Woronin. is one of the worldwide soil-borne diseases. This fungus is an obligate parasitic fungus and cannot be cultured in vitro. The pathogenic fungus can be transmitted through seeds, soil, sick bodies, irrigation water and animal feces. Tumors hinder the normal physiological functions of the roots, slow growth of aboveground plants, wilt due to lack of water, resulting in reduced quality and reduced production, causing huge losses to production. It is known as "cruciferous cancer" and seriously threatens cruciferous Sustainable development of crops. [0003] At present, the prevention and control of clubroot mainly adopts the breeding of disease-resistant varieties, comprehensive agricultural measures, chemical control and biological control...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/22A01P1/00A01P3/00A01P21/00C12R1/07
CPCC12N1/20A01N63/22
Inventor 柴阿丽李宝聚张思雨芮婷婷李磊谢学文石延霞
Owner INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
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