Biocontrol strain for antagonistic phytopathogen and use thereof

A technology of plant pathogenic bacteria and biocontrol strains, applied in plant growth regulators, botanical equipment and methods, and methods based on microorganisms, can solve environmental pollution and other problems, and achieve good antibacterial effects

Active Publication Date: 2014-03-19
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, with the extensive use of chemical pesticides, the problem of environmental pollution has also follow...

Method used

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  • Biocontrol strain for antagonistic phytopathogen and use thereof
  • Biocontrol strain for antagonistic phytopathogen and use thereof
  • Biocontrol strain for antagonistic phytopathogen and use thereof

Examples

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preparation example Construction

[0027] The preparation method of bacterial strain fermentation crude product of the present invention is specifically as follows:

[0028] 1. Obtaining the strain: collect some rice soil samples from Wuxi City, Jiangsu Province, crush the soil samples, and place them in an oven at 50°C to 70°C for 30 to 60 minutes. Weigh the baked soil sample and dissolve it in sterile water, shake it fully to make a soil suspension with different concentration gradients, and apply it on the 2 Cr 2 o 7 On the Gaoshi No. 1 medium plate, cultivate in a constant temperature incubator at 28°C to 32°C for 5 to 10 days. Single colonies were purified on SFM solid medium to obtain Streptomyces griseoruber Y1B (Streptomyces griseoruber Y1B), CCTCC M2013358.

Embodiment 1

[0031] Obtaining the strain: Collect some rice soil samples from Wuxi City, Jiangsu Province, crush the soil samples, and place them in an oven at 60°C for 30 minutes. Weigh 5 g of the baked soil sample and add it to a shaker bottle filled with 45 mL of sterile water, shake for 30 min, and make 10 -1 Concentration of the soil suspension, the soil suspension was thoroughly mixed, diluted in turn to make 10 -2 、10 -3 10 -4 、10 -5 concentrated soil suspension. Then take 10 respectively -2 、10 -3 、10 -4 、10 -5 200 μL of each concentration of soil suspension, spread on the soil supplemented with 0.1% K 2 Cr 2 o 7 Gao's No. 1 medium plate and cultured in a constant temperature incubator at 28°C for 7 days. Single colonies were purified on SFM solid medium to obtain Streptomyces griseoruber Y1B (Streptomyces griseoruber Y1B).

[0032] The components of the above-mentioned various culture media are respectively:

[0033] Gao's No. 1 medium: 20g soluble starch, KNO 3 lg, ...

Embodiment 2

[0036] Preparation of crude product fermented by biocontrol strains: use SFM solid medium to activate the cryopreserved CCTCC M2013358 strain, then inoculate the above-mentioned culture into SFM liquid medium shake flasks, culture at 28°C and 180rpm for 2 days, and use it as a seed solution and then inoculate the seed liquid into the GYM medium shake flask according to the inoculum amount of 2%, and ferment for 7 days under the conditions of 28° C. and 180 rpm. After the fermentation, centrifuge the fermentation broth to collect the fermentation supernatant, extract the fermentation supernatant with an equal volume of ethyl acetate for 3 times, combine and collect the organic phase, distill the organic phase under reduced pressure at 40°C, concentrate and evaporate to dryness, and obtain the crude fermentation product .

[0037] The above GYM medium consists of: 4 g of glucose, 4 g of yeast extract, 10 g of malt extract, distilled water to 1000 mL, pH 7.2.

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Abstract

The invention discloses a biocontrol strain for an antagonistic phytopathogen and use thereof. The strain is streptomyces griseoruber Y1B (Streptomyces griseoruber Y1B) CCTCC No:M2013358. The streptomyces griseoruber Y1B is fermented by adopting a GYM culture medium; a fermentation liquor is collected, extracted, and distilled in vacuum, so as to prepare a fermentation crude product. The fermentation crude product has a good antibacterial effect on rhizoctonia solani and watermelon fusarium oxysporum.

Description

technical field [0001] The invention belongs to the technical fields of biological control and bioengineering, and in particular relates to a biocontrol bacterial strain resistant to plant pathogenic bacteria and its application. Background technique [0002] With the rapid development of agricultural economy, the control of plant diseases and insect pests in agricultural production is increasingly dependent on the wide application of chemical pesticides. However, with the extensive use of chemical pesticides, the problem of environmental pollution has also followed, and people have put forward higher requirements for the prevention and control technology of modern agriculture. Biocontrol pesticides, as a new type of green pesticides, have attracted widespread attention from all over the world in recent years due to their safety, reliability, non-pollution, and good antibacterial effect. Therefore, finding effective biocontrol strains has become a hot spot of increasing conc...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01P1/00C12R1/465
Inventor 王威惠俊渊张雪洪
Owner SHANGHAI JIAO TONG UNIV
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