Method for promoting orchid to grow and preventing diseases

An orchid and seed fungus technology is applied in the field of promoting orchid branching and prolonging flowering period to achieve the effects of prolonging flowering period, ensuring survival and disease prevention, and promoting orchid growth

Active Publication Date: 2014-06-25
HUNAN PLANT PROTECTION INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a method for preventing orchids while preventing and controlling diseases, which can also promote orchid branching, The method of prolonging the flowering period

Method used

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  • Method for promoting orchid to grow and preventing diseases
  • Method for promoting orchid to grow and preventing diseases
  • Method for promoting orchid to grow and preventing diseases

Examples

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Embodiment 1

[0031] Example 1 Preparation Example

[0032] (1) Preservation of the mother seed: The mutant strain T216 of Trichoderma harzianum was cultured with test-tube PDA slant medium;

[0033] (2) Cultivation of seed bacteria: Medium formula: soluble starch 30g, glucose 20g, peptone 5g, KH 2 PO 4 1g, MgSO 4 ·7H 2 O0.5g, CaCO 3 0.25g, (NH4) 2 SO 4 2g, 1000mL water, pH 6.9, dispensed into 500mL Erlenmeyer flasks with a liquid volume of 200mL, sterilized at 115°C for 20 minutes. Under aseptic conditions, the Trichoderma harzianum T216 strain cultured on the sloping medium of the PDA test tube was inserted into an Erlenmeyer flask, and cultured for 60 hours at a culture temperature of 27±1℃ and a rotation speed of 150 rpm. To the prepared seed fungus.

[0034] (3) Preparation of solid culture of T216 strain: Preparation of solid medium for rice bran powder: 500g wheat bran, 450g rice bran, 30g corn flour, (NH 4 ) 2 SO 4 10g, glucose 10g, add water until the water content of the medium is 70-80...

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Abstract

The invention discloses a method for promoting orchid to grow and preventing diseases, and relates to a method for preventing the diseases and promoting orchid branches to grow and prolonging a flowering phase. The method disclosed by the invention comprises the following steps of uniformly mixing a solid state culture and a culture medium into a basin according to the mass ratio of 1 to 50 in orchid plant transplantation, wherein the solid state culture has the spore concentration s 1*10<7>-1*10<8> spore/g and prepared from Trichoderma harzianum T216, and planting an orchid plant, wherein the solid state culture can induce the resistance of the orchid plant to the fungoid diseases such as southern blight, rhizoctonia solani and anthracnose and bacterial soft rot caused by Erwinia carotovara during orchid growth, the plant morbidity is reduced, the disease index is reduced, the orchid is promoted to grow in advance, and the number of blades of the orchid are increased. In addition, the method disclosed by the invention has the advantages that a liquid state culture of the Trichoderma harzianum T216 is regulated into 1*10<5>-1*10<7> spore/mL when the orchid peeps a bud, and the spray treatment is carried out on the orchid plant, so that the branch number of the orchid is increased, and the flowering phase of the orchid is prolonged.

Description

Technical field [0001] The invention belongs to the field of cultivation techniques for promoting the growth of orchids and preventing diseases, and relates to a method for Trichoderma harzianum T216 to prevent orchid diseases and at the same time promote orchid blooming and prolong the flowering period. Background technique [0002] Orchid with purple stems and green leaves, evergreen in all seasons, elegant and elegant leaves, and long-distance blooming fragrance, it is also ranked as the top of the hundred flowers for its noble and elegant temperament, and is widely cultivated by the world. For cultivation under artificial conditions, it is very important to choose a suitable cultivation substrate. Plant materials used for orchid cultivation at home and abroad are generally composed of natural minerals, rural soils, simple substances or mixtures (complexes) of industrial and agricultural organic and inorganic wastes, which basically have water, fertilizer, steam, heat and othe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/04A01P21/00A01N3/00C12N1/14C12R1/885
Inventor 魏林梁志怀张屹李丽辉李萌陈玉荣李卫东
Owner HUNAN PLANT PROTECTION INST
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