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Penicillium composition for promoting plant growth, fungicide and application thereof

A technology that promotes plant growth and composition, applied in the field of microorganisms, can solve the problems of pollutant accumulation, little effect, excessive application of chemical fertilizers, etc., achieve high biological safety, relieve environmental pressure, and promote plant growth.

Pending Publication Date: 2022-03-25
YUNNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to the one-sided pursuit of yield, the excessive application of chemical fertilizers
Long-term excessive application of chemical fertilizers not only directly affects the normal growth of plants and the quality of agricultural products, but also leads to excess nutrients and accumulation of pollutants in the soil, which puts great pressure on the soil environment and brings serious ecological problems
[0003] Due to the characteristics of no pollution, no pollution, low price and good effect, bio-control bacteria are more and more favored by farmers, but the use of microorganisms to promote plant growth still has little effect

Method used

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  • Penicillium composition for promoting plant growth, fungicide and application thereof
  • Penicillium composition for promoting plant growth, fungicide and application thereof

Examples

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Effect test

preparation example Construction

[0032] In the present invention, the preparation method of the metabolite of the Penicillium composition preferably comprises the following steps:

[0033] Activating each Penicillium in the Penicillium composition and then fermenting and culturing to obtain the bacterial liquid of each Penicillium;

[0034] The above bacterial liquid is subjected to high-temperature inactivation, drying and grinding in sequence to obtain the metabolite of the Penicillium composition, that is, the inactivated dry powder of the Penicillium composition.

[0035] In the present invention, when Paecilomyces variotii is activated, it preferably includes sterile water activation and culture medium activation. In the present invention, the process of described sterile water activation is preferably that the bacterium colony of Paecilomyces wansoni (using a bamboo stick to pick a small colony) is inoculated in a 250mL conical flask filled with 100mL sterile water, At a temperature of 28°C and a rotat...

Embodiment 1

[0057] (1) Preparation medium: 5°Bé wort medium (Paecilomyces wannerii): 1.0L of 5°Bé wort, natural pH; PDA medium (Penicillium digitatum): 1.0L of potato extract, glucose 20.0g, natural pH; malt extract culture medium (Penicillium insularia, Penicillium vicinus): malt extract 20.0g, tap water 1.0L, natural pH;

[0058] (2) Activation and fermentation culture: Scrape the colony of Penicillium digitatum (use a bamboo stick to pick a small colony) and inoculate it in a 250mL triangular flask filled with 100mL sterile water, at a temperature of 25°C and a rotational speed of Under the condition of 120 r / min, vibrate for 30 minutes to fully disperse the spore mass, use a pipette gun to draw the spore suspension and place it on a hemocytometer for counting, and finally obtain a concentration of 10 8 cfu / mL spore suspension; the spore suspension obtained above was inoculated in a Erlenmeyer flask containing 100 mL of PDA medium, the inoculation amount was 3% of the volume of the PDA...

Embodiment 2

[0065] Inoculate Bacillus subtilis into a Erlenmeyer flask containing 100mL of NA medium (beef extract 3.0g, peptone 5.0g, NaCl 5.0g, distilled water 1L, pH adjusted to 7.0), the inoculation amount is 3% of the medium volume, 28°C , 125rpm, cultivated for 3 days, the number of bacteria ≥ 10 9 cfu / mL, to obtain Bacillus subtilis fermentation broth;

[0066] Get 100 parts (100 g) of plant-growth-promoting bacterial agent I obtained in Example 1 and 30 parts (30 g) of Bacillus subtilis fermentation broth to obtain plant-growth-promoting bacterial agent II.

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Abstract

The invention belongs to the technical field of microorganisms, and particularly relates to a penicillium composition for promoting plant growth, a fungicide and application of the penicillium composition. The invention provides a penicillium composition for promoting plant growth. The penicillium composition comprises Paecilomyces variotii, Penicillium digitatum, Penicillium islanneum and Penicillium purpureum. The penicillium composition can improve the fresh weight, dry weight, leaf number, plant height, chlorophyll content and hormone content of plants, can effectively promote plant growth, is high in biological safety and free of pollution, and can provide a theoretical basis for production of bio-organic fertilizers in the future.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a penicillium composition for promoting plant growth, a bacterial agent and applications thereof. Background technique [0002] The application of chemical fertilizers is of great significance for increasing crop yields and solving food problems. However, due to the one-sided pursuit of yield, excessive application of chemical fertilizers has been caused. Long-term excessive application of chemical fertilizers not only directly affects the normal growth of plants and the quality of agricultural products, but also leads to excess nutrients and accumulation of pollutants in the soil, which puts enormous pressure on the soil environment and brings serious ecological problems. [0003] Due to the characteristics of no pollution, no pollution, low price and good effect, bio-control bacteria are more and more favored by farmers, but the use of microorganisms to prom...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12N1/20A01N63/36A01N63/30A01N63/22A01P21/00C05F11/08A01B79/00A01G22/40A01G22/00A01G22/45A01G22/05A01G24/20A01G24/00C12R1/80C12R1/79C12R1/125
CPCC12N1/14C12N1/20A01N63/36A01N63/30A01N63/22C05F11/08A01B79/00A01G22/40A01G22/00A01G22/45A01G22/05A01G24/20A01G24/00Y02A40/10
Inventor 牟宗敏陈穗云赵大克李倩倩
Owner YUNNAN UNIV
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