150 results about "Paecilomyces sp" patented technology

The invention discloses a strain of efficient bacillus amyloliquefaciens, and a bacterial agent and application thereof, wherein the Latin name of the bacillus amyloliquefaciens is Bacillus amyloliquefaciens; the strain number is HW05; the accession number of the bacillus amyloliquefaciens in China General Microbiological Culture Collection Center is CGMCC No. 10273. The efficient bacillus amyloliquefaciens provided by the invention has the obvious inhibition effects on fusarium oxysporum, phytophthora capsici, ralstonia solanacearum, pyricularia grisea, colletotrichum gloeosporioides and root-knot nematode. Through the single application of HW05, the efficient prevention and control effects can be achieved on leaf surface disease sweet melon powdery mildew, mango anthracnose diseases andmulberry red rust diseases; the HW05 and paecilomyces lilacinus can be matched for preventing and treating soil-borne banana wilting diseases and banana root-knot nematode diseases, and a good synergyeffect is achieved.

The invention relates to a bacterial strain for preventing and treating tobacco black shank and the bacteria agent thereof, which is characterized in that: the invention adopts paecilomyces lilacinus ZY-19-2, the collection number is CCTCCNO: M207196, as the original bacterial strain to prepare the bacteria agent through a fermentation process, and the fermentation conditions are as follows: 0.8 to 2.0 percent of colloidal chitin is taken as a carbon source, the initial pH value of the fermentation fluid is 5.5 to 7.0, 0.8 to 1.5 percent peptone is taken as a nitrogen source, 0.1 percent Tween-80 is taken as a surfactant, the fermentation time is 48 to 72h, the vaccination amount is 0.8 to 2 percent, and the rotational speed of a shaking bed is 100 to 180r/min. The invention has the following advantages that: 1. the paecilomyces lilacinus strain has fast growth, the producing capacity of chitin is strong; 2. the invention can decompose the body walls of flies and maggots and can grow on the body walls of flies and maggots or a culture medium which is prepared by the decomposition products of the body walls and water; 3. the invention can be colonized at the root of a tobacco plant for resisting the infection of the tobacco black shank; 4. the invention can effectively overcome the antibacterial effect of the soil and maintain the suppression capacity of the tobacco black shank for a long time.

The invention discloses Paecilomyces lilacinus FZ07-9F-5 CGMCC No.2780, microbial inoculum and application thereof. The microbial inoculum is wireworm killing biological microbial inoculum with slow application function, which is prepared by the following steps: using a macromolecular material, namely sodium alginate as basic skeleton, using mycelium and spore mixture of P. lilacinus FZ07-9F-5 CGMCC No.2780 as active components, using infusorial earth as a carrier, and adopting a method of sodium alginate-calcium chloride reaction according to the characteristic of forming gel by the sodium alginate and polyvalent cations to be prepared into the microbial inoculum. Proved by experiments, the microbial inoculum has better wireworm killing efficacy, has control ratio up to between 50 and 60 percent, is comparatively stable, has no noxious chemical residues (innocuous and non-residue), does not pollute agricultural products and environment (does not react with other substances in the environment), and has the characteristics of high cost performance and convenient application. The microbial inoculum plays an important role in control of wireworm diseases of plant roots and knots, and has wide application prospect.

The invention belongs to the technical field of microorganism, in particular to a compound microbial preparation used for directly decomposing crop straws, namely an efficient straw-decomposing inoculant. The efficient straw-decomposing inoculant comprises the following raw materials by weight percentage: 20-30 percent of bacillus subtilis, 10-15 percent of white light gray streptomycete, 5-10 percent of endomycopsis fibuligera, 5-10 percent of candida utilis, 10-15 percent of aspergillus, 10-15 percent of paecilomyces lilacinus, 10-15 percent of trichoderma harzianum and 20-30 percent of thermophilus streptomycete. The efficient straw-decomposing inoculant is organically combined with soil no-tillage, shifting and fallow to achieve the comprehensive purposes of protecting the environment, improving soil, increasing the utilization rate of fertilizers, increasing the quantity and the quality of agricultural products and developing the ecological agriculture.

The invention discloses paecilomyces lilacinus and an application thereof. The provided paecilomyces lilacinus refers to paecilomyces lilacinus E16 with a register number of CGMCC No.5951 in the common microorganism center of the China microorganism culture preservation management committee. According to the paecilomyces lilacinus E16, in potting experiments, the control effect on second-instar banana root-knot nematodes is 86.37%, the growth rate to banana root weight is 37.76%, the growth rate to banana plant height is 56.47%, the growth rate to overground parts of banana is 38.03%; and in area experiments, the control effect on banana root-knot nematodes is 83.03% and the growth rate to banana fruit is 28.05%.

The invention discloses Paecilomyces variotii bacterial strain SJ1 and in particular relates to sea-buckthorn fungus, a preparation of sea-buckthorn endophytic fungi and extract of the sea-buckthorn fungus and use of the sea-buckthorn fungus extract. By utilizing the Paecilomyces variotii bacterial strain SJ1, growth and root development of crop, such as corn, rice are promoted, antifreezing capacity is improved, and yield is increased. The SJ1 bacterial strain is Paecilomyces variotii according to morphological and genetic analysis and identification.

The invention discloses a paecilomyces lilacinus strain and its culture method and use. The paecilomyces lilacinus strain is paecilomyces lilacinus CPLI0708, is preserved in the China general microbiological culture collection center (CGMCC) and has an accession number of CGMCC No.8450. The culture method of the paecilomyces lilacinus strain comprises the following steps of A, bacterial strain activation: carrying out bacterial strain streak cultivation activation on a PDA inclined plane, B, bacterial strain liquid fermentation culture: transferring the activated bacterial strain into a triangular flask with a liquid medium and carrying out shaking culture, and C, bacterial strain solid fermentation culture: mixing a carrier, a nutrition matrix and water according to a volume ratio to form a uniform mixture which is a solid fermentation medium, putting the solid fermentation medium into a fresh-keeping bag, inoculating the solid fermentation medium with a liquid fermentation seed, carrying out stirring to obtain a uniform mixture and carrying out culture. The bacterial strain conidiospore has strong effects of killing sucking-type insects such as greenhouse aphids and whitefly and the fermentation broth has strong effects of killing larvas of soybean cyst nematode and can be used for pollution-free vegetable production.

The invention belongs to the field of biological prevention and control on crop insect damage and provides a microbial preparation for inhibiting root-knot nematode. The preparation is formed by mixing bacterial liquids of streptomyces avermitilis, paecilomyces lilacinus, verticillium chlamydosporium and Bacillus sphaericus and glycerol. The invention also provides a production method and an application effect of the microbial preparation for inhibiting the root-knot nematode. The microbial preparation comprises viable bacteria of which the number is greater than 2,500 millions per milliliter, wherein the viable bacteria comprise the streptomyces avermitilis, the paecilomyces lilacinus, the verticillium chlamydosporium and the Bacillus sphaericus. After the microbial preparation is prepared into soil, eggs of the root-knot nematode are killed by metabolites generated in the continuous growth and reproduction activity process of the viable bacteria, so that a granular structure of the soil is improved, the utilization rate of a fertilizer is improved and an effect of increasing both production and income is achieved.

The present invention provides a strain of entomopathogenic Beauveria bassiana, compositions comprising the entomopathogenic fungi strain or metabolites of the strain, and the use of the entomopathogenic fungi strain and compositions as biological control agents. Methods for the biological control of phytopathogenic insects using an entomopathogenic Beauveria bassiana fungi strain or one or more metabolites thereof, optionally together with other entomopathogenic fungi including fungi selected from strains of Lecanicillium spp., Paecilomyces fumosoroseus, and compositions comprising said fungi or metabolites thereof are also provided.

The invention discloses preparation and application of a soil ecosystem nursing microorganism bacterial fertilizer. The soil ecosystem nursing microorganism bacterial fertilizer contains abundant organic matters and beneficial bacteria, especially paecilomyces lilacinus. The preparation method for the fertilizer mainly comprises the following steps: preparation of a paecilomyces lilacinus strain; enlarged cultivation of a liquid strain used for production; preparation of a medium; addition of the paecilomyces lilacinus strain for enlarged cultivation; secondary fermentation; etc. The microorganism bacterial fertilizer is applied as a base fertilizer to soil according to an amount of 100 to 200 kg/mu, can improve soil, especially soil in a greenhouse, is capable of obviously mitigating harm of a plurality of plant nematode diseases caused by root-knot nematode, soybean cyst nematode, stem eelworm and the like, and alleviates continuous cropping obstacles.

The present invention discloses a strain of Paecilomyces lilacinus for prevention and control of meloidogyne diseases of tomato and grape. The Paecilomyces lilacinus of the present invention is Paecilomyces lilacinus YT08, has the preservation number of CGMCC No.10026, is preserved in the China General Microbiological Culture Collection Center (CGMCC) in November 26, 2014, provides strong prevention and control effects for meloidogyne of tomato and grape, and can be developed as the novel biological prevention and control agent for the agriculture meloidogyne diseases.

The invention discloses a heavy metal bioremediation agent containing alga oligosaccharides and a preparing method of the agent, and relates to the technical field of heavy metal bioremediation application. The heavy metal bioremediation agent containing the alga oligosaccharides comprises, by mass percentage, 5% to 15% of the alga oligosaccharides, 10% to 20% of bacillus subtilis fermented liquid and 65% to 85% of paecilomyces lilacinus solid leavening, wherein the effective viable count is larger than or equal to 2 hundred million CFU/g. The preparation method comprises the steps that the bacillus subtilis fermented liquid is prepared; the paecilomyces lilacinus solid leavening is prepared; and the alga oligosaccharides, the bacillus subtilis fermented liquid obtained in the first step and the paecilomyces lilacinus solid leavening obtained in the second step are mixed, and the heavy metal bioremediation agent containing the alga oligosaccharides is obtained. Bacillus subtilis and paecilomyces lilacinus have the higher tolerance on multiple kinds of heavy metal and can directly adsorb heavy metal ions, and the bacillus subtilis and the paecilomyces lilacinus do combined action with the alga oligosaccharides so as to be capable of passivating the heavy metal in the soil. Preparation is simple, the repairing effect on the heavy metal in the soil is good, and safety and environmental protection are achieved.

The invention relates to an application of an entomogenous fungi liquid fermentation mycelium as a shrimp feed additive, and belongs to the technical field of feed. The entomogenous fungi liquid fermentation mycelium is a Hirsutella sinensis liquid fermentation mycelium, a Paecilomyces gunnii liquid fermentation mycelium, or a Paecilomyces militaris liquid fermentation mycelium. The entomogenous fungi liquid fermentation mycelium is used for improving the growth indexes such as the survival rate, the weight gain rate and the feed utilization rate of white shrimps and increasing immunity.

The invention relates to the technical field of medicine, in particular to a method for preparing a ginsenoside Rd by using the microbial transformation. The invention provides a method for preparinga ginsenoside Rd by using the microbial transformation, including the following steps: adding the ginsenoside into a nutrient solution, and fermenting after Paecilomyces sp being inoculated; obtainingthallines, adding alcohol liquid into the thallines to extract, and obtaining alcohol extract; extracting the alcohol extract with saturated n-butanol; and separating and purifying by chromatographiccolumn. The method disclosed by the invention has high conversion rate, which reaches 70 to 90%, the fermented product ginsenoside Rd has the purity of 98%, moreover, the microbial industrializationpreparation of ginsenoside Rd can be realized, the medicament resource source of ginsenoside Rd can be expanded, and the wide health care market requirement can be met.

The invention discloses epipodium polycyclic aromatic hydrocarbons degrading bacteria and the application thereof. The epipodium polycyclic aromatic hydrocarbons degrading bacteria (paecilomyces variotii) F6 provided by the invention has the function of pipodium polycyclic aromatic hydrocarbons and pipodium polycyclic aromatic hydrocarbons mixture. After the pipodium polycyclic aromatic hydrocarbons is cultured for 30 d in a polycyclic aromatic hydrocarbons compound system, the degrading rate to the polycyclic aromatic hydrocarbons becomes 16.06 percent to 24.57 percent; however, the degrading rate in a single polycyclic aromatic hydrocarbons compound system becomes 10.42 percent to 33.25 percent, wherein, the degrading rate of benzo (k) fluoranthene and benzo (b) fluoranthene is the highest, which respectively accounts for 33.25 percent and 26.44 percent. The strain provided by the invention can supply novel germplasm resource for developing the co-metabolism mechanism study of a plurality of epipodium polycyclic aromatic hydrocarbons and the construction of aromatic hydrocarbons combined pollution water-soil environment repairing technology.

The present invention belongs to the technical field of biological pesticides, and specifically discloses paecilomyces lilacinus PlTS01 strain and application thereof in bemisia tabaci control. The paecilomyces lilacinus PlTS01 strain is isolated and purified from soil in Zhongchuan town Yongdeng County in Gansu Province, and is preserved in China Center for Type Culture Collection (CCTCC) on March 31, 2015, and the accession number is CCTCC No. M2015168. The paecilomyces lilacinus PlTS01 strain has strong bemisia tabaci insecticidal activity, sixth day after the treatment, medial lethal concentration LC50 of B-type bemisia tabaci second instar nymphs is 1.66 * 10<6> spores / mL, and the median lethal time LT50 is 4.35 d (at a dose of 1.0 *10<7> spores / mL). The paecilomyces lilacinus PlTS01 strain shows good prospects for being used as an environmentally friendly microbial pesticide in control of the agricultural important pest-bemisia tabaci.

The invention belongs to the technical field of microbial fermentation, and specifically relates to media for producing Paecilomyces cicadae conidiospores, a culture method thereof, a culture product thereof and application thereof. The media for producing Paecilomyces cicadae conidiospores comprise a solid medium and a liquid medium. The culture method provided in the invention can produce considerable Paecilomyces cicadae conidiospores; the culture product of the method comprises components like polysaccharides, cordycepic acid, adenosine, triterpenoids and a plurality of amino acids, has the functions of regulating immunity, relieving fever and pain, improving renal function, lowering blood pressure, resisting radiation and tumor, reducing blood sugar, etc., and is applicable to the field of human healthy life; considerable spores are generated in the process of fermentation and the generated spores can be used as effective means of biological control, thereby solving the problem that the amount pesticide residues in agricultural products exceed the standard amount from the head stream.

A complex microorganism rhzomorph modifier for saline-alkali soil is characterized by comprising, by weight, 25%-35% of farmland soil, 5%-10% of active functional bacteria, 5%-15% of mineral powder, 25%-30% of organic fertilizer, 15%-20% of inorganic fertilizer and the balance of water. All the components constitute 100% in percentage. The active functional bacteria comprises bacillus megatherium, Paenibacillus kribbensis, Bacillus laterosporus, rhizobium astragali and paecilomyces lilacinus. The mineral powder is the mixture comprising one or more of peat, vermiculite, montmorillonite, zeolite, calcium carbonate, expanded perlite. The fineness of the mineral powder is equal or lower than 200 meshes. The organic fertilizer comprises thoroughly decomposed manure fertilizer, rapeseed cake fertilizer and straw fertilizer. The weight ratio among manure fertilizer, rapeseed cake fertilizer and straw fertilizer is 5.5-7.5:2.5-3.5:0.5-1.5.

The invention relates to the field of plant disease control, in particular to a compound microbial agent, a compound microbial fertilizer and a preparation method and application of the compound microbial agent and the compound microbial fertilizer. The compound microbial agent disclosed by the invention is prepared from the following components in parts by weight: 5 to 7 parts of paecilomyces lilacinus, 6 to 10 parts of bacillus thuringiensis, 4 to 6 parts of bacillus subtilis, 4 to 6 parts of bacillus licheniformis and 4 to 6 parts of trichoderma harzianum. The effective viable count of the paecilomyces lilacinus, the bacillus thuringiensis, the bacillus subtilis, the bacillus licheniformis and the trichoderma harzianum is greater than or equal to 1 * 10 < 10 > CFU/g. According to the invention, a proper microbial agent and an organic fertilizer are compounded to obtain the compound microbial fertilizer. According to the compound microbial agent and the compound microbial fertilizer provided by the invention, the root rot of paris polyphylla can be reduced by 70.3%-78.6% every year, the growth time of overground parts is increased by 30-45 days every year, and the weight of roots and stems is increased by 10.6%-15.5%.

The invention relates to a method for control of tobacco nematode diseases with a composite nematicidal microorganism. The composite nematicidal microorganism is composed of the following four microorganisms: Gliocladium roseum, Paecilomyces lilacinus (Thom.) Samson, Burkholderia arboris and Sarratia entomophilus. Specifically, the Burkholderia arboris is Burkholderia arboris J211, and the preservation number is CCTCC NO:M 2019747. The Burkholderia arboris is a strain obtained by new research, and research finds that the strain has a high control effect on nematodes, and new functions are in continuous research; (2) on the basis of nematode control with the Burkholderia arboris, the other three strains are mixed, and the related volume ratio therein is controlled, so that the results of outstanding control effect and high control rate (shown as the embodiment) are realized; and 3) the composite nematicidal microorganism adopted by the method provided by the invention has obvious capability of improving the nematode disease resistance of field crops, and at the same time can improve commodity quality and yield of crops.

The invention provides a compound fertilizer for increasing sugarcane yield, and relates to the agricultural field. The compound fertilizer is prepared by inoculating a base material with a microbial inoculum after fermentation; the base material includes a backing material; the microbial inoculum includes bacillus megatherium, bacillus mucilaginosus, paecilomyces lilacinus, bacillus laterosporus and bacillus subtilis; the weight ratio of the microbial inoculum before fermentation to the base material is 1 to (1-5). The compound fertilizer for increasing the sugarcane yield can effectively increase the sugarcane yield, increases the sugar content of sugarcane, prevents or controls possible diseases and pests in a sugarcane growth process, and also can maintain or improve soil quality. In addition, the invention also relates to a preparation method of the compound fertilizer for increasing the sugarcane yield. The preparation method is simple, the raw materials are easy to obtain, the price is reasonable, and the preparation method is prone to industrialized mass produced.

The invention relates to a preparation method and an application of a biological microbial inoculum suitable for preventing and treating tomato root-knot nematode diseases, and belongs to the field ofmicroorganisms. The preparation method of the biological microbial inoculum comprises the following steps: preparing a liquid culture medium which comprises the following components in percentage byconcentration: 1.0 percent of sodium nitrate, 0.50 percent of peptone, 0.005 percent of magnesium sulfate heptahydrate, 0.50 percent of ammonium sulfate, 0.05 percent of potassium dihydrogen phosphate, 4.0 percent of glucose and 0.3 percent of jerusalem artichoke straw extract; performing inoculating 1*10 <7>/ml of spore suspension on the prepared liquid culture medium, performing culturing for 7days under the conditions of 28 DEG C and 180r/min, performing filtering, and diluting by one time to obtain the paecilomyces lilacinus metabolite biological microbial inoculum. The biological microbial inoculum prepared by adopting a technology of compounding the organic matter with the nematode killing effect and the culture medium can effectively reduce the occurrence of tomato root knot nematode diseases and promote the growth of tomatoes, and has the advantages of simplicity and convenience in application, batch production, no parasitic influence, high safety and the like; and good application prospects and development potentials are realized in the aspect of relieving tomato root knot nematode diseases.