21 results about "Paecilomyces sp" patented technology

The invention relates to the field of agricultural microbial preparations, in particular to a complex microbial inoculant sustained-release granule for preventing and treating sweet potato stem nematode disease and a preparation method of the complex microbial inoculant sustained-release granule. Comprising the following components in parts by weight: 15-25 parts of paecilomyces lilacinus viable bacterium powder, 10-20 parts of bacillus thuringiensis viable bacterium powder, 10-20 parts of streptomyces viable bacterium powder, 5-15 parts of bacillus subtilis viable bacterium powder, 15-25 parts of charcoal, 5-15 parts of sodium alginate, 5-15 parts of humic acid, 5-10 parts of chitosan, 3-8 parts of calcium silicate, 1-5 parts of mannitol and 1-5 parts of sodium lignin sulfonate. Four microorganisms with complementary functions, namely paecilomyces lilacinus, bacillus thuringiensis, streptomycete and bacillus subtilis, are adopted, the sweet potato stem nematode disease is synergistically controlled through multiple action mechanisms, and the control effect and the stability are remarkably improved.

The invention relates to the technical field of agricultural biology, and discloses a microbial agent for preventing and treating fructus aurantii scab and a preparation method of the microbial agent. The microbial agent comprises the following raw materials in percentage by weight: 10-13% of paenibacillus mucilaginosus; 15%-20% of trichoderma sp. 7%-10% of paecilomyces lilacinus; 5%-8% of bacillus pumilus; 7%-9% of mycorrhizal fungi; 5%-8% of streptomyces; 5%-9% of a coptis chinensis extract; 4%-7% of a sophora flavescens extract; 2%-4% of realgar; 1%-3% of a rheum officinale extract; 10%-13% of soybean meal; 5%-8% of a water-soluble fertilizer; 7%-9% of potassium fulvate; 1%-3% of chitosan; 0.5%-1% of trace elements; 3%-8% of humic acid; the raw materials are subjected to microbial flora amplification culture, plant antibacterial component light-shielding mixing and low-temperature mixing to effectively protect the microbial activity of the microbial agent, and step-by-step operation can avoid damage of high temperature and mechanical shearing to microorganisms, so that the microbial activity of the microbial agent is effectively protected. And the loss of microbial active ingredients is reduced by vacuum drying and low-temperature granulation processes.

The invention discloses a microbial preparation for preventing and treating plant root-knot nematode as well as a preparation method and application thereof, and belongs to the technical field of microbial preparations. The microbial preparation for preventing and treating the plant root-knot nematode is of a double-layer coating structure and comprises an inner layer and an outer layer, the inner layer comprises phaffia rhodozyma fermentation liquor, paecilomyces lilacinus fermentation liquor, bacillus subtilis fermentation liquor, bacillus thuringiensis fermentation liquor, beauveria bassiana fermentation liquor and an inner layer carrier material; the outer layer comprises a streptomyces densely-rotating fermentation liquid and an outer layer carrier material; according to the application, the phaffia rhodozyma, the paecilomyces lilacinus, the bacillus subtilis, the bacillus thuringiensis fermentation liquor, the beauveria bassiana fermentation liquor and the streptomyces dense are compounded, and the strains have a synergistic effect, so that functional complementation is realized; a double-layer coating structure is adapted to acid soil, guarantees the initial activity of the strain and improves the survival efficiency of the strain on one hand, and can prolong the shelf life and the function exerting period on the other hand, so that long-acting pest control is realized.

The application discloses a Paecilomyces hepiali Cs-4 fermentation extract beverage and a preparation method thereof, relates to the technical development field of functional beverages, and comprises the following raw materials in parts by weight: 80-120 parts of a fermentation extract liquid concentrated from a Paecilomyces hepiali Cs-4 fermentation filtrate, 50-120 parts of a medicinal and edible Chinese herbal medicine extract liquid, 5-10 parts of a sweetening agent, and 0.03-0.1 parts of an acidity regulator. The Paecilomyces hepiali Cs-4 fermentation extract beverage provided by the application eliminates the unique bitter taste of Paecilomyces hepiali Cs-4 fermentation products, so that the product has a refreshing taste feature, no bitter taste in the mouth, a sweet aftertaste, and the palatability and market acceptance of the product are significantly improved. Meanwhile, an advanced mild extraction process is adopted, active effective components in the Cs-4 fermentation products are maximally reserved, the large-scale production demand can be met, and the market prospect is wide.

The present invention discloses a strain of Purpureocillium lilacinum for preventing and treating multiple pathogens, and a method for separating and purifying the same, and an application thereof. The Purpureocillium lilacinum strain is named Purpureocillium lilacinum GZUAS951025, and was deposited in the Guangdong Provincial Microbial Culture Collection Center on September 23, 2024, with a deposit number of GDMCC NO.65172. The Purpureocillium lilacinum strain isolated and purified by the present invention has a significant inhibitory effect on Phaseolus vulgaris, Microspores nivalis, Fusarium solani, Fusarium arvense, Fusarium equisetifolia, and Fusarium verticillioides, and can be widely used in preventing and treating multiple pathogenic diseases.

The invention discloses a combined microbial agent for preventing and treating nematodes and application of the combined microbial agent in prevention and treatment of pepper root-knot nematodes. The combined microbial agent comprises the following active components: 1) streptomyces WZSF-1 or a fermentation supernatant thereof, and 2) paecilomyces lilacinus E16 or a fermentation supernatant thereof. The Streptomyces griseorubens WZSF-1 is named as Streptomyces griseorubens WZSF-1, the Streptomyces griseorubens WZSF-1 is classified and named as Streptomyces griseorubens, and the Streptomyces griseorubens WZSF-1 has a preservation number of GDMCC (Microbial Culture Collection Center) No.65368 in Guangdong Province. The Streptomyces griseorubens WZSF-1 is named as Streptomyces griseorubens WZSF-1. Pot experiment results show that E16, WZSF-1 and the compound liquid thereof have promotion effects on plant height, stem diameter, chlorophyll content, overground part fresh weight, root weight and defensive enzyme activity of pepper. Compared with single use of E16 and WZSF-1, the compound treatment has the advantages that the prevention effects are improved by 85.71% and 62.50%.

This invention relates to the field of insect control, specifically to the Paecilomyces lilacinus strain XI-5, and the extraction and identification of crude toxins produced by secondary metabolites of Paecilomyces lilacinus strain XI-5. This invention confirms that the secondary metabolites of Paecilomyces lilacinus strain XI-5 produce cyclosporine C. Cyclosporine C has a stomach poison effect on adult whiteflies. Twenty-four hours after application, the LC50 concentration of cyclosporine C against adult whiteflies was 31.75 µg / ml; compared to the control group, the LC30 concentration of cyclosporine C resulted in a decrease in oviposition / fertility in female whiteflies.

The present invention provides a microbial complex bacterium agent, a preparation method thereof and an application thereof. The complex bacterium agent comprises Pseudomonas orientalis, methylotrophic bacteria, Massilia, Penicillium fructicola, Paecilomyces lilacinus and Streptomyces enniatinus. The present invention also provides an application of the above complex bacterium agent in promoting the formation of a plant rhizosheath structure. By applying the above microorganisms to the soil at the roots of plants, it significantly promotes the formation of the plant rhizosheath, can help improve the ability of plants to tolerate drought stress, can improve the soil fertility, and effectively improves the soil properties.

The application belongs to the technical field of brewing, and relates to application of Paecilomyces wanensis in lactic acid degradation and a method for degrading lactic acid, which comprises the step of adding Paecilomyces wanensis to a fermentation substrate. The application utilizes the mold Paecilomyces wanensis to degrade lactic acid in the fermentation substrate and achieves a good lactic acid degradation effect, further enriches the role of molds in the stacking stage of the solid-state fermentation process of baijiu, provides a reference for the mechanism research of microbial action in the baijiu brewing process, and the strain used is screened from the fermentation environment of fermented grains and does not affect the brewing process.

The invention belongs to the technical field of insect pest control, and particularly discloses a complex microbial inoculant for preventing and treating insect pests and application thereof.The complex microbial inoculant is prepared from, by weight, active ingredients and auxiliary materials, the active ingredients comprise 5-8 parts of bacillus thuringiensis, 4-6 parts of beauveria bassiana, 3-5 parts of metarhizium anisopliae, 2-4 parts of bacillus subtilis, 2-3 parts of bacillus amyloliquefaciens and 1-3 parts of paecilomyces lilacinus, the auxiliary materials comprise the following components in parts by weight: 1-2 parts of a surfactant, 0.5-1 part of a protective agent, 2-3 parts of a nutrient synergist and 30-40 parts of a carrier. According to the complex microbial inoculant, multiple functional microorganisms and synergistic components are reasonably matched, comprehensive and efficient prevention and control of scale insects, red spiders, aphids and fruit borers are achieved, and the complex microbial inoculant is environmentally friendly and safe to durian growth. The defects that durian pest control depends on chemical pesticides, and the control effect of microbial agents is single and unstable in the prior art are overcome.

The invention discloses a combined preparation for preventing and treating morchella diseases and application of the combined preparation, and belongs to the technical field of edible mushroom culture. The combined preparation comprises armillaria luteo-virens polysaccharide F3 and antibacterial peptide Thanatin, and sterile water is used as a carrier. Experimental results show that when the armillaria luteo-virens polysaccharide F3 and the antibacterial peptide Thanatin are combined for use, a remarkable synergistic inhibition effect is achieved on paecilomyces varioti causing white mold of morchella esculenta. In addition, the combined preparation has no obvious negative influence on the growth of mycelia of the morchella esculenta, and shows good safety. Therefore, the invention provides an efficient, safe and environment-friendly prevention and treatment strategy for the white mold of the morchella esculenta, which is beneficial to reducing the use of chemical pesticides, delaying the generation of drug resistance of pathogenic bacteria and promoting the healthy and sustainable development of the morchella esculenta industry.

The invention discloses a broad-spectrum nematicide compounding fungal metabolites and a nano-carrier and a preparation method, and relates to the technical field of insecticides. The broad-spectrum nematicide compounding the fungal metabolite and the nano-carrier is composed of an active component and the nano-carrier, the active component is formed by compounding fermentation broth of paecilomyces lilacinus and pochonia chlamydosporia after extraction, and the nano-carrier is core-shell structure nano-particles encapsulating the nano-carrier. According to the broad-spectrum nematicide containing the composite fungus metabolite and the nano-carrier and the preparation method, the metabolite of paecilomyces lilacinus and the metabolite of pochonia chlamydosporia are scientifically compounded, so that the synergistic effect of active ingredients with different action mechanisms is exerted, the comprehensive poisoning effect on nematodes is remarkably enhanced, the insecticidal spectrum is widened, and the broad-spectrum nematicide has a good application prospect. The chitosan-TPP core and sodium alginate shell double-layer structure provides double protection for active components, and the core ensures high encapsulation efficiency and effective load.

The present invention discloses a microbial composition for preventing and treating white mold disease of Morchella and its use. The microbial composition consists of three strains, namely Streptomyces aurantiogriseus C4-A-9, Agromyces iriomotensis 15-B-11, and Kocuria rosea 17-B-11. The three strains all have good inhibitory effects on Paecilomyces, and after the combination of the three strains, they have a synergistic antibacterial effect on Paecilomyces. The combined microbial composition has a good prevention and treatment effect on white mold disease of Morchella.

The invention relates to the technical field of microbial agents. The invention provides a microbial agent. The microbial agent is prepared from the following components: 14 to 18 parts of bacillus megaterium, 5 to 10 parts of bacillus coagulans, 8 to 13 parts of penicillium baylii fungi, 6 to 11 parts of penicillium oxalicum fungi, 3 to 5 parts of arbuscular mycorrhizal fungi, 2 to 6 parts of bacillus mucilaginosus, 7 to 10 parts of aspergillus niger and 4 to 6 parts of paecilomyces lilacinus. According to the microbial agent disclosed by the invention, various bacteria are matched for use, so that phosphides which cannot be utilized by crops in soil can be effectively promoted to be converted into soluble phosphides which can be utilized, the content of available phosphorus in the soil is increased, the effectiveness of phosphorus is improved, absorption and utilization of the crops are ensured, and the growth and development of the crops are promoted.

The invention belongs to the technical field of organosilicon heat-resistant primer, and particularly relates to a preparation method of organosilicon heat-resistant primer. The invention relates to a preservative. The preservative is prepared by compounding DCOIT and geraniol according to a mass ratio of (2-4): 1. The preservative prepared by compounding the DCOIT and the geraniol has a combined synergistic effect on various moulds such as aspergillus niger, aspergillus flavus, cladosporium cereus, paecilomyces varioti, penicillium citrinum, trichoderma viride, aureobasidium pullulans and alternaria alternata, and compared with single use of the DCOIT, the preservative has the advantages that the inhibition effect on the moulds can be improved; further, the microbial damage resistance of the coating such as the organosilicon heat-resistant primer can be improved, and the shelf life of the coating can be prolonged. Meanwhile, the dosage of DCOIT can be reduced, and harm to the human body is avoided.

The present invention belongs to the technical field of biocontrol agents, and specifically relates to a strain of Paecilomyces lilacinus that has strong pathogenicity to the gypsy moth, a bacterial agent, and its application. The Paecilomyces lilacinus was deposited in the General Microbiology Center of the China Microorganism Culture Collection Administration on November 20, 2024, with a deposit number of CGMCC No. 41635. The present invention determines the lethal concentration and required time of Paecilomyces lilacinus to the gypsy moth indoors, indicating that the strain has strong pathogenicity to the gypsy moth and can be used to prepare biocontrol agents for preventing and controlling pests caused by the gypsy moth. The present invention provides a new resource for the biological control of the gypsy moth and provides a theoretical basis for the development and utilization of highly toxic strains.

The present application relates to the technical field of pesticides, and provides a pesticide composition containing Paecilomyces lilacinus and application thereof in killing root-knot nematodes.The pesticide composition containing Paecilomyces lilacinus comprises fungus powder and modified chitosan in a mass ratio of 5:3-9:1, the modified chitosan is obtained by modifying chitosan with a phosphoric acid compound, and the fungus powder comprises Paecilomyces lilacinus, Trichoderma harzianum, Bacillus cereus and Bacillus polymyxa.Through the technical scheme, the problem of poor control effect of Paecilomyces lilacinus preparation on nematodes in the related art is solved.

The invention belongs to the technical field of microorganisms, and particularly relates to paecilomyces lilacinus as well as a fungicide and application thereof. The invention discloses a novel paecilomyces lilacinum Pli-25, which is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC No.41932, and the 16S rDNA (Reverse Deoxyribose Nucleic Acid) sequence of the paecilomyces lilacinum Pli-25 is as shown in SEQ ID NO: 1. A liquid-state fermentation process and a solid-state fermentation process of the strain are studied, a liquid-state fungicide and a powdery fungicide of the paecilomyces lilacinus strain Purpureocillium lilacinus Pli-25 are developed and obtained, a field test result shows that the prevention and treatment effect on the plant field nematode disease by applying the fungicide is remarkable, a new microbial resource and fungicide are provided for biological prevention and treatment of the plant nematode disease, and the application of the fungicide to the field nematode disease is facilitated. The application prospect is wide.

The invention provides a prevention and treatment agent and method for cape jasmine root knot nematode disease. The prevention and treatment agent is prepared from 1.8% of abamectin, 10% of lythidathion, 41.7% of fluopyram and paecilomyces lilacinus. In the prevention and treatment process, the application amounts of the components of the pesticide are as follows: 243g a.i. / ha of 1.8% abamectin, 115g a.i. / ha of 10% lythidathion, 564g a.i. / ha of 41.7% fluopyram and 5625g a.i. / ha of paecilomyces lilacinus. The medicament and the application method thereof provided by the invention have a good prevention and treatment effect on root knot nematode disease of gardenia, and are suitable for further popularization and application.

The invention discloses a method for preparing an extract rich in ginsenoside Rd by fermenting pseudo-ginseng with paecilomyces farinosus, and belongs to the technical field of microbial fermentation. The method specifically comprises the following steps: crushing and sieving pseudo-ginseng main roots, pseudo-ginseng incisions and pseudo-ginseng stems and leaves, collecting powder with different granularities, and mixing the powder with different granularities to obtain pseudo-ginseng main root coarse powder, pseudo-ginseng incisions coarse powder and pseudo-ginseng stem and leaf coarse powder; preparing pseudo-ginseng powder; preparing a pseudo-ginseng culture medium; the preparation method comprises the following steps: co-culturing paecilomyces farinosus and a pseudo-ginseng culture medium to obtain a fermented suspension culture; the preparation method comprises the following steps: dissolving panax notoginseng into a suspension culture, evaporating the suspension culture to remove water to obtain panax notoginseng plastid, performing reflux extraction on the panax notoginseng plastid, concentrating an extracting solution to obtain extract, dissolving the extract with water, adsorbing an aqueous solution of the extract into resin, eluting, concentrating an eluent, and drying to obtain the extract rich in ginsenoside Rd. The method disclosed by the invention has the advantages of simplicity in operation, no environmental pollution and high efficiency.

The invention discloses a method for producing alcohol by pretreating straws by using an artificial multicellular system, and belongs to the technical field of microbial fermentation degradation. The artificial multi-cell system is composed of trichoderma reesei (Trichoderma reesei), Phanerochaete chrysosporium (Phanerochaete chrysosporium) and Paecilomyces variotii (Paecilomyces variotii), and the artificial multi-cell system is characterized in that the artificial multi-cell system is prepared from the following raw materials: Trichoderma reesei, Phanerochaete chrysosporium (Phanerochaete chrysosporium (Phanerochaete chrysosporium) and Paecilomyces variotii. The method for pretreating the straw to produce the alcohol by using the artificial multicellular system comprises the following steps: (1) crushing the straw, drying and sterilizing; (2) mixing 10 to 20 milliliters of nutrient solution with 4 to 6 grams of sterilized straws; and (3) firstly adding 0.8-1.2 mL of paecilomyces variotii seed solution into a container, mixing 3-5 mL of trichoderma reesei seed solution and 0.8-1.2 mL of phanerochaete chrysosporium seed solution with the substrate in the step (2), covering the bottom of the container with the mixture, sealing, and standing and fermenting at 27-29 DEG C for 10-14 days. Effective degradation of the lignocellulose and ethanol production can be realized through simple pretreatment, complicated pretreatment of the lignocellulose is not needed, and the production cost and the process complexity are reduced.