Bacillus DY26-004 and application thereof to prevention and control of plant pathogenic fungi

A Bacillus, plant pathogenic technology, applied in the application, plant growth regulators, botanical equipment and methods, etc., can solve the problem of few microorganisms, and achieve simple fermentation medium, broad antifungal spectrum, and obvious antibacterial effect. Effect

Active Publication Date: 2016-01-13
THIRD INST OF OCEANOGRAPHY STATE OCEANIC ADMINISTATION +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Most of the microbial strains currently used for biological control come from land, and the screening of land microorgani...

Method used

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  • Bacillus DY26-004 and application thereof to prevention and control of plant pathogenic fungi
  • Bacillus DY26-004 and application thereof to prevention and control of plant pathogenic fungi
  • Bacillus DY26-004 and application thereof to prevention and control of plant pathogenic fungi

Examples

Experimental program
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Effect test

Embodiment 1

[0024] Example 1: Isolation and screening of Bacillus sp. DY26-004

[0025] The present invention relates to bacillus ((Bacillussp.) DY26-004, which is isolated by the applicant from the sediment samples collected during the 26th Oceanic Science Expedition in China. The bacterium grows on LB medium and cultures at 28°C for 24 hours. The bacteria are drop-shaped, light white, and the colonies are large, see figure 1 .

Embodiment 2

[0026] Embodiment 2: Bacillus sp. (Bacillus sp.) DY26-004 strain identification

[0027] Bacillus DY26-004 was identified by 16SrRNA gene sequencing. The sequence of the ocean-derived strain DY26-00416SrDNA was amplified using universal primers. The sequence of the forward primer 27F was: AGAGTTTGATCCTGGCTCAT, and the sequence of the reverse primer 1492R was: ACGGCTACCTTGTTACGACTT. The total DNA of Bacillus spp. DY26-004 was used as the PCR amplification template for PCR reaction. The reaction conditions were: denaturation at 94°C for 1 min; annealing at 55°C for 1 min; extension at 72°C for 1.5 min, 30 cycles. The amplified gene was sent to a sequencing company for sequencing. After obtaining the 16S rDNA sequence of the strain, the sequence was compared and analyzed by the National Center for Biotechnology Information (NCBI) and the nucleic acid data in GeneBank ( http: / / ncbi.nlm.nih.gov / blast ). The results showed that the sequence similarity between DY26-00416SrDNA seq...

Embodiment 3

[0028] Embodiment 3: the mensuration of phytopathogenic fungi antimicrobial spectrum

[0029] Activate the bacillus DY26-004 obtained in Example 1 on LB liquid medium, then transfer it into 50mL LB culture medium with 1% inoculum, shake and cultivate it at 28°C at 200r / min for 2 days, filter and sterilize after centrifugation to prepare The sterile fermentation supernatant was used for later use. The inhibitory activity of Bacillus DY26-004 against 10 plant pathogenic fungi was determined by filter paper method.

[0030] Use a sterile puncher with a diameter of 6 mm to make the test pathogenic fungi into bacterial blocks, place them in the center of the potato solid medium (PDA) plate, and culture them at 28°C for 1 to 2 days, and wait for the test pathogenic fungi to grow. After the filaments diffuse and grow, place a number of sterilized double-layer filter paper sheets with a diameter of 6mm around each bacterial block. The filter paper is 0.5-1cm away from the edge of the...

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Abstract

The invention relates to bacilli, in particular to a bacillus DY26-004 and application thereof to prevention and control of plant pathogenic fungi. A preservation number of the Bacillus sp.DY26-004 is CCTCC NO:M 2015406. The bacillus DY26-004 has a strong inhibiting effect on various plant pathogenic fungi such as fusaria, colletotrichum musae, paecilomyces variotii, sclerotinia scleotiorum, alternaria longipes, trichoderma viride, rhizoctonia solani, alternaria alternata, botrytis cinerea and colletotrichum gloeosporioides, and has good biological prevention and control effects in pot experiments for prevention and control of sclerotinia scleotiorum and fusarium graminearum, wherein control efficiencies are 72% and 65% respectively. The bacillus DY26-004 which is a nontoxic, non-pathogenic and environment-friendly bacillus is a useful resource for research and development of microbial pesticides and has a potential application prospect.

Description

technical field [0001] The invention relates to bacillus, in particular to a strain of bacillus DY26-004 and its application in the preparation of fermentation supernatant for preventing and controlling plant pathogenic fungi. Background technique [0002] Plant diseases have always been one of the important factors affecting agricultural production. The long-term and extensive use of chemical pesticides has not only led to the resistance of pathogenic bacteria, but also the pesticide residues are harmful to humans, animals and the environment. Biological control is a method of using beneficial organisms or their products to suppress or eliminate harmful organisms. Due to its advantages of non-toxicity, pollution-free and resistance to pesticides, it has become a new direction in the research of plant disease control (Calvo et al. 2007). Bacteria are widely used in research on plant disease control due to their short growth cycle, easy regulation of metabolism, and large-sc...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/02A01P3/00C12P1/04C12R1/07
Inventor 陈新华吴小君郭文斌
Owner THIRD INST OF OCEANOGRAPHY STATE OCEANIC ADMINISTATION
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