Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for detection of paecilomyces variotii

A technology of Paecilomyces variotii and its detection method, which is applied in the field of detection of Paecilomyces variotii, can solve the problem of difficult specific and rapid detection of Paecilomyces variotii, and the specific gene region of Paecilomyces variotii has not been elucidated And other issues

Inactive Publication Date: 2011-06-01
KAO CORP
View PDF9 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the specific gene region of Paecilomyces variotii has not been elucidated in these prior art
Therefore, there is a problem that it is difficult to detect Paecilomyces variotii specifically and rapidly

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for detection of paecilomyces variotii
  • Method for detection of paecilomyces variotii
  • Method for detection of paecilomyces variotii

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0181] Example 1 Determination of the base sequence of the β-tubulin gene of Paecilomyces variabilis by PCR

[0182] The test bacteria were placed in potato dextrose agar slant medium and cultured in the dark at 25°C for 7 days. Use Gen Torukun TM (Produced by Takara Bio Co., Ltd.) to extract DNA from bacteria. For the PCR amplification of the target site, PuRe TaqTM Ready-To-Go PCR Beads (produced by GE Health Care UK LTD) were used, and Bt2a (5'-GGTAACCAAATCGGTGCTGCTTTC-3', sequence number 34), Bt2b (5'-ACCCTCAGTGTAGTGACCCTTGGC) were used as primers -3', serial number 35) (Glass and Donaldson, Appl Environ Microbiol 61: 1323-1330, 1995). The amplification conditions were that the denaturation temperature of the length of the β-tubulin portion was 95°C, the annealing temperature was 59°C, and the extension temperature was 72°C, and 35 cycles were performed. The PCR product was purified using Auto SegTM G-50 (manufactured by Amersham Pharmacia Biotech). The PCR product was l...

Embodiment 2

[0204] Example 2 Detection of Paecilomyces variabilis by LAMP method

[0205] (1) Design and synthesis of primers

[0206] Based on the specific nucleotide sequence regions of Paecilomyces variegii in the above-mentioned Example 1 (SEQ ID NOs. 1 to 4 and 22 to 33), oligonucleotides represented by the nucleotide sequences described in SEQ ID NOs: 10 to 15 were designed The composition of the primers was commissioned by E Genome order (Fujitsu System Solution Co., Ltd., serial numbers 10 and 11, 5 pmol grade; serial numbers 12 and 13, 40 pmol grade; serial numbers 14 and 15, 20 pmol grade, all column purified products) synthesis and purchase Into.

[0207] (2) Preparation of the test object

[0208] As fungi used for evaluating the effectiveness of the designed primers, namely Paecilomyces variabilis and other heat-resistant bacteria, the fungi shown in Table 3 were used. As these fungi, those purchased and used are fungi kept in the Center for Fungal Medicine, Chiba University School...

Embodiment 3

[0223] Example 3 Detection of Paecilomyces variabilis by LAMP method

[0224] (1) Design and synthesis of primers

[0225] Based on the specific base sequence region of Paecilomyces variabilis (Sequence Nos. 1 to 4 and 22 to 33) in the above-mentioned Example 1, an oligonucleotide represented by the base sequence described in SEQ ID NO: 16 to 21 was designed The composition of the primers was commissioned by E Genome order (Fujitsu System Solution Co., Ltd., serial numbers 16 and 17, 5 pmol grade; serial numbers 18 and 19, 40 pmol grade; serial numbers 20 and 21, 20 pmol grade, all column purified products) synthesis and purchase Into.

[0226] (2) Preparation of the test object

[0227] As fungi used to evaluate the effectiveness of the designed primers, that is, Paecilomyces variabilis, other heat-resistant bacteria and general molds, the fungi shown in Table 4 were used. As these fungi, those purchased and used are fungi kept in the Center for Fungal Medicine, Chiba University Sc...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
temperatureaaaaaaaaaa
Login to View More

Abstract

Disclosed is a method for detecting Paecilomyces variotii. In the method, Paecilomyces variotii is identified by using a nucleic acid represented by any one of the following nucleotide sequences (a) and (b): (a) a partial nucleotide sequence of ss-tubulin gene, which is depicted in any one of SEQ ID NO:1 to SEQ ID NO:4 and SEQ ID NO:22 to SEQ ID NO:33, or a sequence complementary to the partial nucleotide sequence; and (b) a nucleotide sequence which has the deletion, substitution or addition of one or several nucleotides in a nucleotide sequence depicted in any one of SEQ ID NO:1 to SEQ ID NO:4 and SEQ ID NO:22 to SEQ ID NO:33 and can be used for the detection of Paecilomyces variotii, or a sequence complementary to the nucleotide sequence.

Description

Technical field [0001] The present invention relates to a detection method of Paecilomyces variotii. Background technique [0002] Paecilomyces variotii is an incomplete fungus widely distributed in nature. During its growth cycle, Paecilomyces variabilis forms asexual spores called chlamydospores composed of double cell walls. It is known that the chlamydospores of Paecilomyces variabilis have extremely high compressive resistance, and they can survive and proliferate even under the sterilization conditions of heat and reagents to kill common fungi, which is the cause of mold production. Therefore, Paecilomyces variabilis is regarded as a harmful bacteria in the food industry and cosmetics industry and has received attention. For this reason, the detection and identification of Paecilomyces variabilis is extremely important in establishing an anti-corrosion and anti-mildew system. [0003] The detection and identification methods of Paecilomyces variabilis are based on morpholo...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/00C12N15/09C12Q1/68
CPCC07K14/37C12Q1/6895C12Q2600/16
Inventor 细谷幸一中山素一德田一矢口贵志弘佑介
Owner KAO CORP
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com