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Devices and kits for propagating or storing microorganisms and methods for their preparation and use

A microbiological and physical technology, applied in the field of kits, preparation of the device, proliferation or storage of microorganisms, to achieve the effect of rapid detection

Active Publication Date: 2021-04-02
3M INNOVATIVE PROPERTIES CO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Disadvantages of using natural gum include that some organisms are able to metabolize the sugar chains of natural gum, leading to at least some liquefaction of the dish

Method used

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  • Devices and kits for propagating or storing microorganisms and methods for their preparation and use
  • Devices and kits for propagating or storing microorganisms and methods for their preparation and use
  • Devices and kits for propagating or storing microorganisms and methods for their preparation and use

Examples

Experimental program
Comparison scheme
Effect test

Embodiment approach 1

[0064] Embodiment 1 is a device for multiplying or storing microorganisms. The device comprises a) a first layer comprising a first portion of a surface of the first layer to which a first water-swellable gelling agent comprising a first clay is attached. The device also includes b) a second layer separable from the first layer and comprising a first portion of the surface of the second layer to which a second water-swellable gelling agent is attached.

Embodiment approach 2

[0065] Embodiment 2 is the device of embodiment 1, wherein the first layer further comprises a second portion of the surface.

Embodiment approach 3

[0066] Embodiment 3 is the device of embodiment 1 or 2, wherein the second layer further comprises a second portion of the surface.

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Abstract

Devices for the propagation or storage of microorganisms are provided including a first layer that has a first portion of a surface of the first layer, to which a first water-swellable gelling agent comprising a first clay is affixed. The devices further include a second layer that is separable from the first layer and has a first portion of a surface of the second layer, to which a second water-swellable gelling agent is affixed. Methods for detecting and enumerating at least one microorganism in a sample are provided. The methods include providing a device, separating the first layer from the second layer, adding an aliquot of a sample containing at least one microorganism onto the first or second water-swellable gelling agent to form an inoculated device, laminating the first layer back to the second layer, and incubating the inoculated device. Kits and methods of making the devices are also provided.

Description

technical field [0001] The present disclosure relates to devices for culturing and detecting microorganisms, including water-swellable gelling agents. The present disclosure also relates to methods of making such devices, kits, and methods of using such devices to propagate or store microorganisms. Background technique [0002] Dehydrated microbial culture devices are known to rely on guar and other natural gums as cold water soluble gelling agents. Natural gum works extremely well in most applications and has excellent gelling properties compared to other cold water soluble polymers. Disadvantages of using natural gum include that some organisms are able to metabolize the sugar chains of natural gum, leading to at least some liquefaction of the dish. In addition, natural gum tends to break down at the levels of ionizing radiation typically used to decontaminate or sterilize petri dishes. [0003] Therefore, there remains a need for devices for multiplying or storing micr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/00C12M1/34C12Q1/04
CPCC12M23/04C12M23/20C12M25/06C12M41/36C12M45/22C12Q1/04A61L2/081A61L2/087C12Q1/06
Inventor E·D·布鲁蒂内尔A·J·扬A·J·斯坦纳斯
Owner 3M INNOVATIVE PROPERTIES CO