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Serratia marcescens LTH-2 as well as screening method and application thereof

A technology of Serratia marcescens and LTH-2, applied in chemical instruments and methods, microbial-based methods, biochemical equipment and methods, etc., can solve the problems of lack of algicidal bacteria research, freshwater bloom pollution purification treatment, etc. , to achieve the effect of eliminating microcystis blooms, high efficiency and saving consumption

Inactive Publication Date: 2012-02-01
SOUTHEAST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to provide a kind of Serratia marcesens (Sarratia marcesens) LTH-2, which solves technical problems such as the lack of algicidal bacteria research for water bloom treatment in the prior art and the purification treatment of fresh water bloom pollution.

Method used

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  • Serratia marcescens LTH-2 as well as screening method and application thereof
  • Serratia marcescens LTH-2 as well as screening method and application thereof
  • Serratia marcescens LTH-2 as well as screening method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0079] Example 1 Screening and Morphological Observation of Serratia marcesens (Sarratia marcesens) LTH-2

[0080] Serratia marcesens (Sarratia marcesens) LTH-2 was isolated from Taihu Lake in Wuxi City, Jiangsu Province, the People's Republic of China, and has been preserved in the China Center for Type Culture Collection with the preservation number CCTCC M 2011209.

[0081] The raw material used to screen Serratia marcesens (Sarratia marcesens) LTH-2 is Taihu Lake water.

[0082] The specific screening steps are as follows:

[0083] (1) Prepare alginolytic bacteria solid separation medium: beef extract 5g, peptone 10g, NaCl 5g, agar powder 2g, distilled water 100mL, pH7.2;

[0084] (2) Gradually dilute the water samples from Taihu Lake, 10-fold dilution to form a concentration gradient, take 0.1mL of samples of each concentration and spread them on a solid medium plate, cultivate for 24 hours to form colonies, and then pick a single colony to separate and purify to obtain ...

Embodiment 2

[0090] The identification of embodiment 2 Serratia marcescens (Sarratia marcesens) LTH-2

[0091] This embodiment establishes a rapid (using the Biolog microbial identification system, the identification result can be obtained within 16 hours), reliable and accurate method for the classification and identification of microorganisms for the cultivated Serratia marcesens LTH-2. way. The specific identification steps are as follows:

[0092] A.Biolog microbial identification system identification

[0093] The Biolog Microbial Identification System tests the ability of microorganisms to utilize or oxidize compounds in identification plates. The test will produce a characteristic purple hole pattern to form a metabolic fingerprint. The system software will automatically compare it with the database. If a suitable match can be found, an identification result can be obtained. For specific methods, please refer to MicroLogTM System, Release 4.2 instruction manual of Biolog Company....

Embodiment 3

[0108] Preparation and structure identification of the red pigment of embodiment 3 bacterial strains

[0109] Serratia marcescens LTH-2 was used as the production strain to prepare the red metabolite prodigiosin produced by it. The specific steps for preparing prodigiosin were as follows:

[0110] (1) Preparation of mother seed: culture sourdough yeast in beef extract peptone, inoculate dark red strain LTH-2 on beef extract peptone slant medium, and use this strain as sour yeast strain.

[0111] (2) Fermentation culture production: inoculate and produce fermented yeast species in fermentation medium for fermentation production, the fermentation conditions are: aerated culture, and the production temperature is 30°C. The fermentation medium is a liquid medium, and the medium formula is: 5g of beef extract, 10g of peptone, 5g of NaCl, 100mL of distilled water, and the pH is controlled at 7.2.

[0112] The fermentation process is divided into three stages, and the cultivation is...

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Abstract

The invention discloses Serratia marcescens LTH-2 the preservation number of which is CCTCCM2011209. The Serratia marcescens LTH-2 has the capabilities of dissolving Microcystis and generating prodigiosin. The Serratia marcescens LTH-2 can be used for producing algae metabolin prodigiosin and can eliminate Microcystis water bloom and the environment pollution caused by the water bloom of Microcystis.

Description

technical field [0001] The invention belongs to the technical field of Serratia marcescens screening, and in particular relates to Serratia marcescens LTH-2 and its screening method and application. Background technique [0002] With the rapid development of social economy and the acceleration of social industrialization, people's unreasonable life and production methods have led to a large amount of nutrient salts such as nitrogen and phosphorus in waters, resulting in increasingly serious eutrophication of global waters. The direct harm brought by eutrophication of water body is the occurrence of algae bloom. In the warm season, algae proliferate in large numbers, often floating on the water surface or scattered in the water in a floating state to form pieces of blue-green algae blocks or thin layers of algae, that is, "bloom". Over the past ten years, "water blooms" have appeared frequently in waters such as Dianchi Lake and Taihu Lake. When "water blooms" appear, due t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/02C02F3/34C12R1/43
Inventor 浦跃朴杨飞尹立红吴巍
Owner SOUTHEAST UNIV
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