39 results about "P. mirabilis" patented technology

A method and a composition for treatment of pulmonary bacterial infections caused by gram-negative bacteria suitable for treatment of infection caused by Escherichia coli, Klebsiella pneumoniae, Klebsiella oxytoca, Pseudomonas aeruginosa, Haemophilus influenzae, Proteus mirabilis, Enterobacter species, Serratia marcescens as well as those caused by Burkholderia cepacia, Stenotrophomonas maltophilia, Alcaligenes xylosoxidans, and multidrug resistant Pseudomonas aeruginosa, using a concentrated formulation of aztreonam, or a pharmaceutically acceptable salt thereof, delivered as an aerosol or dry powder formulation.

The invention relates to nucleotide special to an Internal transcribed spacer (ITS for short) of a 16S rRNA-23S rRNA gene in Proteus mirabilis, in particular to oligonucleotide special to the ITS in the Proteus mirabilis and application thereof. The invention also provides a PCR detection reagent taking an oligonucleotide pair as a primer and a detection method thereof. The detection method utilizes the PCR reagent to detect the Proteus mirabilis in human body and the environment, is simple, convenient and quick, has good specificity and high sensitivity, can be used in the fields of supervision and detection of food and clinical samples, detection of pathogen in the food, microbial classification and epidemiological investigation and so on, and has deep social benefit and large economic benefit.

The present invention discloses a Proteus mirabilis strain and a method for producing S-equol through daidzein conversion by using the Proteus mirabilis strain, wherein the strain is preserved in the China Center for Type Culture Collection on November 13, 2011, and a preservation number is CCTCC M 2011390. In the prior art, the existing technology is difficult to prepare the S-equol through conversion. With the present invention, the problem in the prior art is solved, and a single function microorganism strain for directly converting a prochiral compound daidzein into a chiral compound S-equol and a preparation method thereof are provided; and compared with the strictly anaerobic equol conversion strains and the mixing bacteria conversion in the previous reports, the Proteus mirabilis LH-52 strain of the present invention is a facultative anaerobe, and is more suitable for industrial production.

The invention discloses a specific nucleic acid marking sequence of proteus mirabilis, a PCR detecting method and the application. The aim of the invention is to provide the specific nucleic acid marking sequence of the proteus mirabilis, a qualitative and quantitative PCR detecting method of the proteus mirabilis and the application in preparing qualitative and quantitative PCR detecting kits of the proteus mirabilis. The specific nucleic acid marking sequence of the proteus mirabilis is one of following nucleic acid sequences: firstly, a DNA sequence of SEQ ID NO: 1 in the table, secondly, a restricted DNA sequence of the SEQ ID NO: 1 in the table, which has the homology more than 90% and is the specific nucleic acid sequence of the proteus mirabilis, and thirdly, a nucleic acid sequence which can cross-breed with the restricted DNA sequence of the SEQ ID NO: 1 in the table under the high-stringent condition. The invention can be used in qualitative and quantitative molecular detection of the proteus mirabilis (including the detecting methods of ordinary PCR, quantitative PCR, chips, hybrid and the like) and has broad application prospect.

The invention relates to a gene chip for detecting important pathogenic bacteria in an aquatic product and a kit thereof. The gene chip comprises a solid phase carrier and an oligonucleotide probe; the oligonucleotide probe comprises one or more of the following nucleotide sequences: (1) DNA (Deoxyribonucleic Acid) sequences selected from proteus mirabilis 1, proteus vulgaris, salmonella, vibrio parahaemolyticus, vibrio cholerae, Listeria monocytogenes, staphylococcus aureus, streptococcus pyogenes, vibrio vulnificus, bacillus cereus cluster, Shigella and pathogenic Y. enterocolitica ail gene; (2) complementary DNA sequences of the DNA sequences selected in the (1); (3) complementary RNA (Ribonucleic Acid) sequences of the DNA sequences in the (1) or (2). The kit comprises the gene chip. The gene chip and the kit can be used for detecting the important pathogenic bacteria in an aquatic product, are convenient to operate, high in precision and strong in repeatability.

The invention provides a multiplex PCR (Polymerase Chain Reaction) primer group for avian pathogenic escherichia coli, pasteurella multocida, proteus mirabilis, pseudomonas aeruginosa, salmonella andstaphylococcus aureus, a multiplex PCR detection kit comprising the primer group, and a multiplex PCR detection method using the primer group. The primer group comprises primer pairs PhoA-F and PhoA-Rfor specifically amplifying a gene phoA of the avian escherichia coli, primer pairs KMT-F and KMT-R for specifically amplifying a gene KMT1 of the pasteurella multocida, primer pairs AtpD-F and AtpD-R for specifically amplifying a gene AtpD of the proteus mirabilis, primer pairs PETA-F and PETA-R for specifically amplifying a gene PETA of the pseudomonas aeruginosa, primer pairs InvA-F and InvA-Rfor specifically amplifying a gene InvA of the salmonella, and prier pairs NuC-F and NuC-R for specifically amplifying a gene nuc of the staphylococcus aureus.

The present invention relates to new nucleic acid sequences derived from the ITS region, between the 16S and 23S ribosomal ribonucleic acid (rRNA) or rRNA genes, to be used for the specific detection and / or identification of Proteus species, in particular of Proteus mirabilis, Proteus vulgaris and / or Proteus penneri in a biological sample. The present invention relates also to a method for the specific detection and / or identification of Proteus species, in particular Proteus mirabilis, Proteus vulgaris and / or Proteus penneri, using said new nucleic acid sequences derived from the ITS (Internal Transcribed Spacer) region. It relates also to nucleic acid primers to be used for the amplification of said spacer region of Proteus species in a sample.

The invention discloses a method for high-throughput screening of alpha-keto acid high-producing strains, and belongs to the technical field of bioengineering. The method comprises the steps that protein engineering modification is carried out on the L-amino acid deaminase PmiLAAD derived from proteus mirabilis, a high-throughput screening method is used, a recombinant strain and a strain mutant of the L-amino acid deaminase PmiLAAD containing proteus mirabilis are cultured in a 96-well plate, transfer induction is carried out, cells of the L-amino acid deaminase PmiLAAD containing proteus mirabilis are centrifugally collected to convert L-amino acids (L-valine, L-methionine and L-phenylalanine) to generate corresponding alpha-ketonic acid, developing is carried out by utilizing 2, 4-dinitrophenylhydrazine, a light absorption value is read at 520nm, shake flask verification is carried out on a bacterial strain with a large light absorption value, and screening is carried out to obtain the bacterial strain with significantly improved alpha-keto acid yield.

The invention discloses multi-PCR detection primers for detecting four sheep pathogenic bacteria and a detection method. The primers include Klebsiella pneumoniae upstream and downstream primers, proteus mirabilis upstream and downstream primers, escherichia coli upstream and downstream primers and salmonella upstream and downstream primers. According to the detection method, the primers are adopted to perform multi-PCR amplification reaction with Klebsiella pneumoniae DNA, proteus mirabilis DNA, escherichia coli DNA and salmonella DNA as templates, PCR amplification reaction products are added into loading buffer, the mixture is put into agarose gel containing ethidium bromide, and electrophoresis detection is performed to verify the specificity of the primers. The detection method has the advantages that molecular detection with high sensitivity can be achieved just through conventional instruments, and the detection sensitivity of the method is high; meanwhile, no complex operation system is needed, and detection can be performed under common laboratory conditions; besides, the method is easy and fast to implement, high in sensibility and the like.

The method is suitable for the technical field of bacteria detection, the invention discloses a triple TaqMan Real-Time PCR detection method for simultaneously distinguishing proteus mirabilis(P. mirabilis), proteus vulgaris (P.vulgaris) and proteus penesei (P.pennerei), and a kit. The primers and nucleotide sequences of proteusbacillus vulgaris are predicted through computer software, and three pairs (six) of primers capable of being used for simultaneously detecting three proteusbacillus vulgaris and nucleotide sequences of three probes are disclosed. The method has the characteristics of strong specificity, high sensitivity and accurate and reliable result. According to the method, the proteusbacillus vulgaris can be identified as a specific species while the proteusbacillus vulgaris isdetected, the defect that only proteus mirabilis can be distinguished in existing proteusbacillus vulgaris detection is overcome, the proteusbacillus vulgaris detection process is greatly simplified,the workload of operators is reduced, the detection period is shortened, and a powerful technical support can be provided for detecting the proteusbacillus vulgaris in the industries of medical treatment, medical detection and the like.

Provided is an information providing method for diagnosing Parkinson's disease by measuring the amount of any one target selected from the group consisting of a Proteus mirabilis strain, a metabolite produced by the Proteus mirabilis strain, and α-synuclein in a biological sample of a subject; a composition comprising a Proteus mirabilis strain as an active ingredient for fabricating a Parkinson's disease animal model; a method for fabricating a Parkinson's disease animal model, the method comprising a step for administering a Proteus mirabilis strain to an animal excluding human; and a method for screening a Parkinson's disease medicine, the method comprising a step for administering a candidate drug of the Parkinson's disease medicine to the Parkinson's disease animal model and a step for observing the degree of mitigation of Parkinson's disease symptoms to determine the treatment effect of the candidate drug on Parkinson's disease.

The invention discloses a compound bacteria culture composition, which includes proteus morganii culture, proteus mirabilis culture, filamentous Streptomyces culture, bacillus thuringiensis culture and candida mycoderma bacteria culture. The compound bacteria culture composition is prepared by mixed-culturing proteus morganii culture, proteus mirabilis culture, filamentous Streptomyces culture, bacillus thuringiensis culture and candida mycoderma bacteria culture according to part by weight; under the condition of tolerating toxicity of heavy metals, the composition releases antibiotic and canplay safe and effective purifying effect to sewage commonly polluted by pathogenic bacteria and heavy metals.

The invention discloses a proteus mirabilis preparation for treating domestic sludge, and a preparation method and application thereof, belonging to the technical field of domestic sludge treatment. The proteus mirabilis preparation is prepared by culturing the proteus mirabilis by mixing the wormcast extracting solution and other nutritional ingredients, and then the proteus mirabilis preparationis sprayed into the domestic sludge to treat the sludge, the moisture content of the treated sludge can be reduced to 30-50%, the reduction and harmless are obvious, and the fertilizer preparation and the soil conditioner requirements are satisfied.

The present invention discloses a Proteus mirabilis phage RDP-SA-16033. The host of the Proteus mirabilis phage is Proteus mirabilis S5. The phage may form a plaque having a diameter of 4-6 mm on a double-layer plate. When observed through an electron microscope, the phage has a head in polyhedral cubic symmetry, is coated by nucleic acid, has a diameter of about 70 nm, has a tail of about 150 nm long, has a tail sheath, has a neck connected to the head and the tail, and belongs to tailed virales myovirus division. The present invention further provides a production process of the phage. After centrifugation of value-added liquid, a titer of the phage is increased by membrane concentration, and then residual host and other infectious microbes in value-added are effectively removed by a ceramic membrane and a 0.22 μm polyethersulfone filter membrane. Meanwhile, the phage is reserved to the utmost extent.

The invention discloses a compound bacteria culture composition, which includes proteus morganii culture, proteus mirabilis culture, filamentous Streptomyces culture, bacillus thuringiensis culture and candida mycoderma bacteria culture. The compound bacteria culture composition is prepared by mixed-culturing proteus morganii culture, proteus mirabilis culture, filamentous Streptomyces culture, bacillus thuringiensis culture and candida mycoderma bacteria culture according to part by weight; under the condition of tolerating toxicity of heavy metals, the composition releases antibiotic and canplay safe and effective purifying effect to sewage commonly polluted by pathogenic bacteria and heavy metals.

The invention provides an oily sludge degradation strain Proteus mirabilis SB and an application thereof. The strain is Proteus mirabilis, and is preserved in the China General Microbiological Culture Collection Center on January 25, 2021, and the preservation number is CGMCC NO.21729. The Proteus mirabilis SB is separated from oily sludge, so that the strain quickly becomes a dominant flora after entering the oily sludge, the degradation of petroleum hydrocarbons is effectively promoted, the physical properties of soil are improved, and the biological activity of the soil is enhanced. The strain can metabolize a biological surfactant in the growth and reproduction process, and the degradation rate of microorganisms on petroleum hydrocarbons in the oily sludge is increased. The strain Proteus mirabilis SB disclosed by the invention can degrade petroleum pollutants in the oily sludge within a short time, and the degradation rate of total petroleum hydrocarbon (TPH) in the oily sludge within two weeks reaches 70.5% or above; and the strain Proteus mirabilis SB has a remarkable degradation effect on the aromatic hydrocarbons in the oily sludge, and the degradation rate of the aromatic hydrocarbons in the total petroleum hydrocarbons in the oily sludge within two weeks reaches 69.2%.

The invention discloses a proteus mirabilis phage vB_PmiP_pPm05, and an application of the proteus mirabilis phage vB_PmiP_pPm05 in the inhibition or killing of proteus mirabilis in food. The proteus mirabilis phage vB_PmiP_pPm05 is high in amplification efficiency, can be massively amplified in a short time, is wide in temperature and acid-base tolerance range, can effectively prevent and control food pollution caused by proteus mirabilis, and does not influence the texture and flavor of food.

The invention provides a multiplex PCR primer set for avian pathogenic Escherichia coli, Pasteurella multocida, Proteus mirabilis, Pseudomonas aeruginosa, Salmonella and Staphylococcus aureus, and multiplex PCR comprising the primer set A detection kit and a multiplex PCR detection method using the primer set, the primer set includes: a primer pair PhoA‑F and PhoA‑R for specific amplification of the phoA gene of avian Escherichia coli; a specific amplification of the Pasteurella multocida KMT1 gene The primer pair KMT‑F and KMT‑R; the primer pair AtpD‑F and AtpD‑R of the specific amplification Proteus mirabilis AtpD gene; the primer pair PETA‑F and PETA‑R of the specificity expansion Pseudomonas aeruginosa PETA gene; Primer pair InvA‑F and InvA‑R for specific amplification of Salmonella InvA gene; primer pair NuC‑F and NuC‑R for specific amplification of Staphylococcus aureus nuc gene.

The invention discloses a strain of Proteus vulgaris derived from human intestinal flora, a new application of its fermented liquid and the components contained therein. The strain, named HA‑3151, is effective against common Gram-positive and Gram-negative pathogens such as Staphylococcus aureus, Pseudomonas aeruginosa, Klebsiella pneumoniae, Proteus mirabilis, Escherichia coli, enteritis Salmonella has obvious inhibitory effect. The bacterial strain, its fermented liquid and its components can be used in medicine, food manufacturing and research and development.

The application discloses a pathogen-specific nucleic acid gene of Proteus mirabilis and a detection method, the nucleotide sequence of the pathogen-specific nucleic acid gene is at least a part of any sequence in the sequence table Seq ID No:4-Seq ID No:10 or a complementary sequence thereof. A PCR reaction is carried out by taking clinical sample DNA as a template, a forward primer in amplification primers used in the PCR reaction is SEQ ID NO:1, a reverse primer is SEQ ID NO:2, a target sequence of crRNA used in cooperation of the primer pair is indicated in SEQ ID NO:3, and detection is carried out by using LbCas12a, crRNA and ssDNA-reporter. The pathogen-specific nucleic acid fragment provided by the application can be used for rapid identification of the pathogen of Proteus mirabilis, and has high accuracy and short detection period when applied in clinic.

The invention belongs to the technical field of synthesis of polysaccharide conjugate vaccines, and relates to a proteus mirabilis O3a O-antigen tetrasaccharide repeating unit containing an adamantane skeleton, an antigen and a polysaccharide conjugate vaccine. The active ingredients of the polysaccharide conjugate vaccine provided by the invention comprise a subject and an object, and the subject and the object are mutually combined through non-covalent interaction; the subject comprises a compound of beta-cyclodextrin and carrier protein, and the guest comprises maltotriose containing an adamantane skeleton or a proteus mirabilis O3a O-antigen containing an adamantane skeleton. The proteus mirabilis O3a O-antigen tetrasaccharide repeating unit containing the adamantane skeleton is successfully synthesized with high stereoselectivity and excellent yield by adopting a [2 + 1 + 1] graded assembly strategy, and the research can lay a firmer theoretical and practical foundation for research and development of proteus mirabilis resistant vaccines.

The invention discloses a specific nucleic acid marking sequence of proteus mirabilis, a PCR detecting method and the application. The aim of the invention is to provide the specific nucleic acid marking sequence of the proteus mirabilis, a qualitative and quantitative PCR detecting method of the proteus mirabilis and the application in preparing qualitative and quantitative PCR detecting kits ofthe proteus mirabilis. The specific nucleic acid marking sequence of the proteus mirabilis is one of following nucleic acid sequences: firstly, a DNA sequence of SEQ ID NO: 1 in the table, secondly, a restricted DNA sequence of the SEQ ID NO: 1 in the table, which has the homology more than 90% and is the specific nucleic acid sequence of the proteus mirabilis, and thirdly, a nucleic acid sequence which can cross-breed with the restricted DNA sequence of the SEQ ID NO: 1 in the table under the high-stringent condition. The invention can be used in qualitative and quantitative molecular detection of the proteus mirabilis (including the detecting methods of ordinary PCR, quantitative PCR, chips, hybrid and the like) and has broad application prospect.

The invention discloses an organic waste water compound microbial treatment agent and a use method thereof. The organic waste water compound microbial treatment agent is obtained by respectively culturing bacilli, bifidobacterial, enterococcus faecalis, bacillus cereus, Yangshi proteus mirabilis, pseudomonas putida and enterobacter cloacae and then performing mixing. The use method comprises the steps that the compound microbial treatment agent is added into organic waste water, and the addition quantity is 10 to 1000 mg / L.

The invention belongs to the field of biology, and discloses a proteus mirabilis homologous recombination system expression plasmid which comprises a pBR322 replication starting point, a resistance gene, an arabinose inducible promoter and a proteus mirabilis-derived homologous recombination operon. The homologous recombination operon is used for coding a TPM66325 protein, an EPM66325 protein, a single-chain binding protein SSB and a fake release protein. The expression plasmid has relatively high recombination efficiency in proteus mirabilis GDMCC 66325 and can also play a recombination role in a proteus mirabilis standard strain ATCC35659, and the expression plasmid is obtained by knocking out a related gene bcsB formed by a hemolysin related gene hpmA and a biological membrane of the proteus mirabilis GDMCC 66325 and directly knocking in a heterologous gene after a related gene cluster is adjusted by flagellum. The effectiveness of the expression plasmid in recombinant operation of proteus mirabilis is verified; meanwhile, the invention further discloses a proteus mirabilis homologous recombination system, application and a gene knockout / knockin method.

The invention discloses application of proteus mirabilis outer membrane vesicle in preparation of a medicine for preventing or treating osteolytic diseases. The proteus mirabilis outer membrane vesicle inhibits miR96-5p expression and promotes Abca1 expression, thereby inhibiting an MAPK / ERK pathway and causing osteoclast differentiation to be blocked; the proteus mirabilis outer membrane vesicle induces release of MPT-related cytochrome c to cause a mitochondrial structure to be damaged, increase production of active oxygen and cause increase of osteoclast apoptosis. The proteus mirabilis outer membrane vesicle not only significantly inhibits differentiation and functions of RANKL-induced osteoclasts in vitro, but also can improve bone metabolism imbalance caused by OVX and bone erosion caused by CII in vivo, and provides a new idea for preventing or treating the osteolytic diseases.

The invention belongs to the technical field of biology, and discloses proteus mirabilis, application of metabolite of the proteus mirabilis and anti-tumor medicine.Tests find that thalli and the metabolite of the proteus mirabilis have a good inhibiting effect on part of tumors, and it is found in the in-vivo test process that the metabolite of the proteus mirabilis has a good inhibiting effect on part of tumors. The metabolite of the proteus mirabilis has a very prominent inhibition effect on melanoma.

The application relates to the technical field of rapid detection methods, in particular to a rapid detection method of proteus mirabilis which is not for disease diagnosis or treatment purposes, first, the RPA reaction system and the CRISPR / Cas12a reaction system are respectively placed at the bottom of a PCR reaction tube and the inner side of the tube cover, so that the competition between CRISPR / Cas12a and RPA is avoided, the reaction rate and sensitivity are ensured, and because the tube cover does not need to be opened, the risk of nucleic acid aerosol pollution is also avoided; second, the RPA provided by the application specifically amplifies target DNA through preferred primers, the CRISPR / Cas12a specifically recognizes a target sequence through preferred crRNA, and under preferred reaction conditions, through the double screening of the RPA and the CRISPR / Cas12a, the proteus mirabilis can be rapidly, sensitively and specifically detected, the reliability is high, the cross reactivity is low, and the application is convenient to popularize.

The invention provides a primer probe combination for detecting Klebsiella aerogenes, a Klebsiella oxytoca complex and proteus mirabilis as well as application and a kit of the primer probe combination, and relates to the technical field of biology. Primer probes are respectively designed aiming at a conserved gene (chbr gene) of klebsiella aerogenes, a conserved gene (fusC gene) of a klebsiella oxytoca complex and a conserved gene (gntr gene) of proteus mirabilis. The designed primer probe has strong specificity and high sensitivity, can accurately detect the Klebsiella aerogenes, the Klebsiella oxytoca complex and the proteus mirabilis in the same amplification system, improves the detection efficiency, and provides reference for auxiliary diagnosis of related bacterial infection.

Described herein are compositions, methods, and kits for detecting wound pathogens. One embodiment described herein is primer pairs and probes for individual, simultaneous, or sequential multiplex polymerase chain reaction (PCR) based assays for the detection of Acinetobacter baumannii, Bacteroides fragilis, Clostridium perfringens, Enterobacter cloacae, Enterococcus faecalis, Enterococcus faecium, Escherichia coli, Klebsiella pneumoniae, Klebsiella Oxytoca, Klebsiella aerogenes, Proteus mirabilis, Proteus vulgaris, Pseudomonas aeruginosa, Serratia marcescens, Staphylococcus aureus, Streptococcus agalactiae, Streptococcus dysgalactiae, Streptococcus pyogenes, Streptococcus anginosus, Staphylococcus lugdunensis, Providencia stuartii, Stenotrophomonas maltophilia, Morganella morganii, Citrobacter koseri, Citrobacter freundii, Citrobacter braakii, Peptoniphilus asaccharolyticus, Peptoniphilus harei, Peptoniphilus ivorii, Peptostreptococcus anaerobius, Finegoldia magna, Clostridium septicum, Paeniclostridium sordellii (Clostridium sordellii), Clostridium novyi A, B, Clostridium histolyticum, Staphylococcus epidermidis, Staphylococcus haemolyticus, Corynebacterium striatum, Candida albicans, Candida auris, Candida glabrata, Candida krusei, Candida parapsilosis, or Candida tropicalis.