New uses of proteus vulgaris

A technology of Proteus vulgaris and pathogenic bacteria, which is applied in the field of Proteus vulgaris and its fermentation broth, and can solve problems such as the lack of antibacterial activity of Proteus vulgaris

Active Publication Date: 2021-11-26
BEIJING UNIV OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, no antibacterial activity of Proteus vulgaris has been reported

Method used

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  • New uses of proteus vulgaris
  • New uses of proteus vulgaris
  • New uses of proteus vulgaris

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] The separation and identification process of the Proteus vulgaris HA-3151 bacterial strain with antibacterial activity that the present invention relates to is as follows:

[0016] (1) Separation of HA-3151

[0017] The strains of the present invention are isolated from feces of human origin.

[0018] A Take the middle part of the feces and put it into sterile physiological saline, and incubate at 150 r / min for 1 hour to obtain a mixed bacterial solution.

[0019] B Dilute the mixed bacterial solution to 10 -4 、10 -5 、10 -6 、10 -7 , draw each dilution and inoculate it in the improved Gaoshi No. 1 liquid medium (soluble starch 20.0g, KNO 3 1.0g, K 2 HPO 4 0.5g, MgSO4 7H 2 O 0.5g, NaCl 0.5g, FeSO 4 ·7H 2 (00.01g, agar 20.0g, potassium dichromate 50.0mg, distilled water 1000mL), 37 ℃, 150r / min culture 12-24h, obtain mixed flora; Gained mixed flora is inoculated on nutrient agar culture by dilution coating method base (beef extract 3.0g, peptone 10.0g, NaCl 5.0...

Embodiment 2

[0031] Determination of broad-spectrum antibacterial activity of Proteus vulgaris HA-3151

[0032] A. Inoculate the stable-growing strain HA-3151 in the nutrient broth medium, cultivate it at 37°C and 150r / min for 8h, and transfer it to 300mL fermentation medium (soybean peptone 10.0g, peptone 2.0g) with 10% inoculum size. , glucose 20.0g, soluble starch 5.0g, yeast extract 2.0g, NaCl 4.0g, K 2 HPO 4 0.5g, MgSO 4 ·7H 2 O 0.5g, CaCO 3 2.0g, distilled water 1000mL) in an Erlenmeyer flask, 37°C, 150r / min, shaker culture for 5 days to obtain the fermentation broth.

[0033] B Prepare nutrient agar medium, pour the plate, and place it upside down in a constant temperature incubator at 37°C overnight after it solidifies, and evaporate the surface moisture; the activated indicator strains (Staphylococcus aureus, Pseudomonas aeruginosa, bacillus, Klebsiella pneumoniae, Escherichia coli, Salmonella enteritidis) bacterial solution concentration adjusted to 0.5 McFarland turbidity...

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Abstract

The invention discloses a strain of Proteus vulgaris derived from human intestinal flora, a new application of its fermented liquid and the components contained therein. The strain, named HA‑3151, is effective against common Gram-positive and Gram-negative pathogens such as Staphylococcus aureus, Pseudomonas aeruginosa, Klebsiella pneumoniae, Proteus mirabilis, Escherichia coli, enteritis Salmonella has obvious inhibitory effect. The bacterial strain, its fermented liquid and its components can be used in medicine, food manufacturing and research and development.

Description

technical field [0001] The invention belongs to the field of microbes, and relates to a strain of Proteus vulgaris producing antibacterial active substances, its fermented liquid and the new application of the components contained therein. Background technique [0002] Colonization resistance is the basis for the joint action of intestinal flora to maintain the homeostasis of the intestinal environment. The secretion of antibacterial substances is the key for intestinal bacteria to resist the invasion of foreign pathogens and their colonization in the intestine. The specific antimicrobial substances are poorly understood. Proteus vulgaris is one of the representative strains of the genus Proteus. It is an opportunistic pathogenic bacterium that colonizes the human intestinal tract. It is symbiotic with the host under normal circumstances. The detection rate of diseases caused by simple Proteus vulgaris infection is low (Drzewiecka D , Microb Ecol, 2016, 72:741-758.). In 20...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12R1/37
CPCA23L3/3571A61K35/74A23L33/135A61P31/04C12N1/205C12R2001/37Y02A50/30
Inventor 王如峰李德利赓迪
Owner BEIJING UNIV OF CHINESE MEDICINE
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