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A complex bacterial culture composition resistant to heavy metal ion toxicity and its preparation method and application

A technology of culture and composite bacteria, applied in the direction of biochemical equipment and methods, methods based on microorganisms, chemical instruments and methods, etc., can solve the problems of not being able to improve the environment well, easy to interact with each other, and differences in growth conditions and nutritional requirements Huge and other problems, to achieve the effect of safe and effective purification

Active Publication Date: 2020-10-30
江苏世邦生物工程科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the use of chemical reagents is likely to cause secondary pollution to the water body, and it is not easy to carry out follow-up treatment; in addition, among the biological improvement measures currently used, the bacteria with the most obvious application effects mainly include photosynthetic bacteria, bacillus, lactic acid bacteria, and actinomycetes etc., because they belong to different species, photosynthetic bacteria are photoautotrophic, while other species are chemoheterotrophic, bacillus are spore-forming bacteria, while other species are non-spore-forming bacteria, the growth conditions and nutritional requirements vary greatly, At present, most of the microbial preparations for improving water bodies are mixed multi-effect strains. The compound strain preparations with different functions and growth conditions are not only easy to interact with each other in the growth period, but also unable to play a role in the later process of water body treatment. To a good effect of improving the environment

Method used

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  • A complex bacterial culture composition resistant to heavy metal ion toxicity and its preparation method and application
  • A complex bacterial culture composition resistant to heavy metal ion toxicity and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Raw material components: 40 cultures of Proteus morinella ATCC 25830, 40 cultures of Proteus mirabilis ATCC 14153, 2 cultures of Streptomyces filamentosa ATCC 23958, 2 cultures of Bacillus thuringiensis ATCC 10792 and Candida 5 copies of ATCC 24565 culture.

[0021] Preparation and application: (1) inoculate the various bacterial species in the raw material components on the slanted medium, respectively, and expand the culture by shaking flask or solid medium to obtain the culture of each bacterial species; wherein, the shaking flask culture The conditions are: temperature 27°C, rotation speed 160rpm, culture for 1 day, pH is 6.6; the medium inoculated on the slant surface of each strain in the raw material components is: Proteus Morsei inoculation medium: Gao's No. 1 medium (soluble starch 20g / L, sodium chloride 0.5g / L, potassium nitrate 1.0g / L, potassium dihydrogen phosphate trihydrate 0.5g / L, magnesium sulfate heptahydrate 0.75g / L, magnesium iron sulfate heptahydrate...

Embodiment 2

[0024] Raw material components: 50 cultures of Proteus morinella ATCC 25829, 50 cultures of Proteus mirabilis ATCC 29906, 5 cultures of Streptomyces filamentosa ATCC 31568, 5 cultures of Bacillus thuringiensis ATCC 39756 and Candida 10 cultures of ATCC 204276.

[0025] Preparation and application: (1) inoculate the various bacterial species in the raw material components on the slanted medium, respectively, and expand the culture by shaking flask or solid medium to obtain the culture of each bacterial species; wherein, the shaking flask culture The conditions are: temperature 30°C, rotating speed 180rpm, culture for 3 days, pH is 7.0; the medium inoculated on the slant surface of each strain in the raw material components is: Proteus Morsei inoculation medium: Gao's No. 1 medium (soluble starch 20g / L, sodium chloride 0.5g / L, potassium nitrate 1.0g / L, potassium dihydrogen phosphate trihydrate 0.5g / L, magnesium sulfate heptahydrate 0.75g / L, magnesium iron sulfate heptahydrate 0....

Embodiment 3

[0028] Ingredients: 45 cultures of Proteus morinella ATCC 23548, 45 cultures of Proteus mirabilis ATCC 12453, 4 cultures of Streptomyces filamentosa ATCC 31568, 3 cultures of Bacillus thuringiensis ATCC 39756 and Candida 7 copies of ATCC 204276 culture.

[0029] Preparation and application: (1) inoculate the various bacterial species in the raw material components on the slant medium respectively, and expand the culture by shaking flask or solid medium respectively to obtain the culture of each bacterial species, wherein, the shaking flask culture The conditions are: temperature 28°C, rotation speed 170rpm, culture for 2 days, pH is 6.8; the medium inoculated on the slant surface of each strain in the raw material components is: Proteus Morsei inoculation medium: Gao's No. 1 medium (soluble starch 20g / L, sodium chloride 0.5g / L, potassium nitrate 1.0g / L, potassium dihydrogen phosphate trihydrate 0.5g / L, magnesium sulfate heptahydrate 0.75g / L, magnesium iron sulfate heptahydrate 0...

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Abstract

The invention discloses a compound bacteria culture composition, which includes proteus morganii culture, proteus mirabilis culture, filamentous Streptomyces culture, bacillus thuringiensis culture and candida mycoderma bacteria culture. The compound bacteria culture composition is prepared by mixed-culturing proteus morganii culture, proteus mirabilis culture, filamentous Streptomyces culture, bacillus thuringiensis culture and candida mycoderma bacteria culture according to part by weight; under the condition of tolerating toxicity of heavy metals, the composition releases antibiotic and canplay safe and effective purifying effect to sewage commonly polluted by pathogenic bacteria and heavy metals.

Description

technical field [0001] The present invention relates to a microbial composition, its preparation method and application, in particular to a compound bacteria culture composition used for the treatment of sewage polluted by pathogenic bacteria and heavy metals, and its preparation method and application. Background technique [0002] The pathogenic microorganisms in the water body mainly come from human and animal feces and domestic sewage pollution, which can be divided into three categories: bacteria, viruses and protozoa; there are various ways for pollutants to enter the water source. Particulate matter settles into natural water sources under the action of gravity, and the solid and liquid wastes in domestic wastewater enter the water body directly with sewage pipes; in addition to pollutants such as pathogenic microorganisms that easily cause public safety hazards, industrial water separation wastewater It carries a large amount of heavy metal pollution sources into the...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/16C02F3/34C12R1/01C12R1/465C12R1/07C12R1/72C02F101/20
CPCC02F3/34C02F3/347C02F2101/20C12N1/16C12N1/20
Inventor 谢悦波姚竣耀刘才群
Owner 江苏世邦生物工程科技有限公司
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