Oily sludge degrading strain Proteus mirabilis SB and application thereof

A technology of oily sludge and bacterial strains, applied in the direction of water/sludge/sewage treatment, bacteria, water pollutants, etc., can solve problems such as research limitations, achieve the effects of enhancing biological activity, increasing degradation rate, and changing properties

Active Publication Date: 2021-07-23
XI'AN PETROLEUM UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Proteus mirabilis is a kind of Proteus genus, which often exists in human feces and is an opportunistic pathogen. There are many studies on this strain, but most of its sources are in animals or their feces. also mostly limited to biomedical aspects

Method used

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  • Oily sludge degrading strain Proteus mirabilis SB and application thereof
  • Oily sludge degrading strain Proteus mirabilis SB and application thereof
  • Oily sludge degrading strain Proteus mirabilis SB and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0036] Degradation and remediation test of actual oily sludge in Yanchang Oilfield by Proteus mirabilis SB

[0037] Oily sludge from Yanchang Oilfield was taken, air-dried, ground, passed through a 2mm sieve, and set aside. The strain Proteus mirabilis SB was inoculated into the broth medium, and the Erlenmeyer flask was placed in a shaker at 120rpm and activated at 30°C for 24h. Weigh 20kg of the above air-dried, ground and sieved oily sludge, inoculate the bacterial solution in the oily sludge with an inoculation amount of 10wt%, in order to increase the air permeability of the oily sludge during fermentation, add 1kg Add ammonium chloride 200g, potassium dihydrogen phosphate 45g at the same time, make C:N:P=90:5:1, and control moisture content 20%, temperature 25 ℃, pile up and ferment after waiting to stir evenly, pile up thickness 50 -80cm, stir and replenish water every 3 days, and the treatment cycle is 60 days.

[0038] During the microbial treatment, samples were ta...

Embodiment 2

[0044] Degradation and restoration test of oily sludge in Yanchang Oilfield by Proteus mirabilis SB

[0045] Oily sludge from Yanchang Oilfield was taken, air-dried, ground, passed through a 2mm sieve, and set aside. The strain Proteus mirabilis SB was inoculated into the broth medium, and the Erlenmeyer flask was placed in a shaker at 120rpm and activated at 30°C for 24h. Weigh 20 kg of the above air-dried, ground and sieved oily sludge, and inoculate the bacterial solution in the oily sludge with an inoculation amount of 5 wt%. In order to increase the air permeability of the oily sludge in the fermentation process, add 1 kg of Sawdust, add 200g of ammonium chloride and 45g of potassium dihydrogen phosphate at the same time, make C:N:P=90:5:1, and control the moisture content to 20%, the temperature is 25°C, and after stirring evenly, accumulate and ferment, and the accumulation thickness is 50- After 60 days of biodegradation treatment, the oily sludge was tested, and it w...

Embodiment 3

[0047] Degradation and restoration test of oily sludge in Yanchang Oilfield by Proteus mirabilis SB

[0048] Oily sludge from Yanchang Oilfield was taken, air-dried, ground, passed through a 2mm sieve, and set aside. The strain Proteus mirabilis SB was inoculated into the broth medium, and the Erlenmeyer flask was placed in a shaker at 120rpm and activated at 30°C for 24h. Weigh 20 kg of the above air-dried, ground and sieved oily sludge, inoculate the bacterial solution in the oily sludge with an inoculation amount of 10 wt%, in order to increase the air permeability of the oily sludge in the fermentation process, add 1 kg of Sawdust, add 200g of ammonium chloride and 45g of potassium dihydrogen phosphate at the same time, make C:N:P=90:5:1, and control the moisture content to 30%, the temperature is 25°C, after stirring evenly, stack and ferment, and the stack thickness is 50~ 80cm, stirring and replenishing water every 3 days, after 60 days of biodegradation treatment, the...

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Abstract

The invention provides an oily sludge degradation strain Proteus mirabilis SB and an application thereof. The strain is Proteus mirabilis, and is preserved in the China General Microbiological Culture Collection Center on January 25, 2021, and the preservation number is CGMCC NO.21729. The Proteus mirabilis SB is separated from oily sludge, so that the strain quickly becomes a dominant flora after entering the oily sludge, the degradation of petroleum hydrocarbons is effectively promoted, the physical properties of soil are improved, and the biological activity of the soil is enhanced. The strain can metabolize a biological surfactant in the growth and reproduction process, and the degradation rate of microorganisms on petroleum hydrocarbons in the oily sludge is increased. The strain Proteus mirabilis SB disclosed by the invention can degrade petroleum pollutants in the oily sludge within a short time, and the degradation rate of total petroleum hydrocarbon (TPH) in the oily sludge within two weeks reaches 70.5% or above; and the strain Proteus mirabilis SB has a remarkable degradation effect on the aromatic hydrocarbons in the oily sludge, and the degradation rate of the aromatic hydrocarbons in the total petroleum hydrocarbons in the oily sludge within two weeks reaches 69.2%.

Description

technical field [0001] The invention relates to the technical field of biochemical treatment of oily sludge; in particular, it relates to an oily sludge degrading bacterial strain Proteus mirabilis SB and its application. Background technique [0002] The biological treatment of oily sludge mainly uses the metabolism of microorganisms to degrade petroleum hydrocarbons and finally convert them into CO 2 and H 2 O. The more representative ones are the composting method and the bioaugmentation method. The former is to fully stir the oily sludge and the consumable substances required for biological treatment, then stack them in rows, and use an aeration truck to periodically stir them to make them fully react. The latter is to add microbial agents that efficiently decompose petroleum hydrocarbons to oily sludge. These microorganisms use petroleum hydrocarbons as a carbon source for metabolism, and use the metabolic activities of microorganisms to assimilate and degrade petrole...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C02F3/02C12R1/01C02F101/32
CPCC02F3/34C02F3/02C02F2101/32Y02W10/10
Inventor 柯从玉秦芳玲张群正孙妩娟张洵立王嗣昌
Owner XI'AN PETROLEUM UNIVERSITY
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