Proteus mirabilis preparation for treating domestic sludge, and preparation method and application thereof
A technology for Proteus mirabilis and domestic sludge, which is applied in the field of Proteus mirabilis preparations and their preparation, can solve the problems of high operating costs, large one-time investment, and high equipment investment, and achieves high organic value, stable properties, and improved cultivation methods. The operation process is simple and easy to operate
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Embodiment 1
[0034] A kind of preparation method that is used for the preparation of Proteus mirabilis preparation that the preferred embodiment of the present invention provides for treating domestic sludge, concrete steps are as follows:
[0035] S1. Soak fresh vermicompost in water for 30 minutes, take the soaking solution, the ratio of vermicompost to water is 0.38g: 1mL;
[0036] S2. Add the soaking solution obtained in step S1, the Proteus mirabilis strain and the culture medium into the culture container according to the volume ratio of the medium, the soaking liquid and the Proteus mirabilis strain of 5:0.1:0.01, and place at 12°C Cultivate at high temperature for 12 hours to obtain the culture mother solution;
[0037] S3. Disperse the culture mother liquor obtained in step S2 in the culture bucket, and then add the culture medium into the culture bucket, the volume ratio of the culture medium and the culture mother liquor is 10:0.2, and cultivate at room temperature for 12 hours ...
Embodiment 2
[0040] A kind of preparation method that is used for the preparation of Proteus mirabilis preparation that the preferred embodiment of the present invention provides for treating domestic sludge, concrete steps are as follows:
[0041] S1. Soak fresh vermicompost in water for 30 minutes, take the soaking solution, the ratio of vermicompost to water is 0.50g: 1mL;
[0042] S2. Add the soaking solution obtained in step S1, the Proteus mirabilis strain and the culture medium according to the volume ratio of the culture medium, the soaking liquid and the Proteus mirabilis strain of 5.2:0.1:0.01 into the culture container, and place at 13°C Cultivate at high temperature for 12 hours to obtain the culture mother solution;
[0043]S3. Disperse the culture mother liquor obtained in step S2 in the culture bucket, and then add culture medium into the culture bucket. The volume ratio of the culture medium to the culture mother liquor is 10.2:0.2, and cultivate at room temperature for 12 ...
Embodiment 3
[0046] A kind of preparation method that is used for the preparation of Proteus mirabilis preparation that the preferred embodiment of the present invention provides for treating domestic sludge, concrete steps are as follows:
[0047] S1. Soak fresh vermicompost in water for 30 minutes, take the soaking solution, the ratio of vermicompost to water is 0.45g: 1mL;
[0048] S2. Add the soaking liquid obtained in step S1, the Proteus mirabilis strain and the culture medium into the culture container according to the volume ratio of the culture medium, the soaking liquid and the Proteus mirabilis strain being 5.5:0.1:0.01, and place at 15°C Cultivate at high temperature for 12 hours to obtain the culture mother solution;
[0049] S3. Disperse the culture mother liquor obtained in step S2 in the culture bucket, and then add culture medium into the culture bucket. The volume ratio of the culture medium and the culture mother liquor is 10.5:0.2, and cultivate at room temperature for ...
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