Compound bacteria culture composition capable of tolerating toxicity of heavy metal ion and its preparation method and application

A technology of culture and compound bacteria, applied in biochemical equipment and methods, microbial-based methods, chemical instruments and methods, etc. Safe and effective purification effect

Active Publication Date: 2018-04-27
江苏世邦生物工程科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the use of chemical reagents is likely to cause secondary pollution to the water body, and it is not easy to carry out follow-up treatment; in addition, among the biological improvement measures currently used, the bacteria with the most obvious application effects mainly include photosynthetic bacteria, bacillus, lactic acid bacteria, and actinomycetes etc., because they belong to different species, photosynthetic bacteria are photoautotrophic, while other species are chemoheterotrophic, bacillus ar...

Method used

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  • Compound bacteria culture composition capable of tolerating toxicity of heavy metal ion and its preparation method and application
  • Compound bacteria culture composition capable of tolerating toxicity of heavy metal ion and its preparation method and application

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Experimental program
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Effect test

Embodiment 1

[0020] Raw material components: 40 parts of Proteus morgii ATCC 25830 culture, 40 parts of Proteus mirabilis ATCC 14153 culture, 2 parts of Streptomyces filamentous ATCC 23958 culture, 2 parts of Bacillus thuringiensis ATCC 10792 culture and Candida 5 copies of ATCC 24565 culture.

[0021] Preparation and application: (1) Inoculate each bacterial strain in the raw material components on a slant medium, respectively expand culture through shake flasks or solid medium to obtain cultures of various strains; wherein, shake flask culture Conditions are: temperature 27 DEG C, rotating speed 160rpm, culture 1 day, pH is 6.6; The culture medium that each bacterial strain slant inoculates in the raw material component is respectively: Proteus morgii inoculation medium: Gaoshi No. 1 medium (soluble starch 20g / L, sodium chloride 0.5g / L, potassium nitrate 1.0g / L, potassium dihydrogen phosphate trihydrate 0.5g / L, magnesium sulfate heptahydrate 0.75g / L, magnesium sulfate heptahydrate ferric...

Embodiment 2

[0024] Raw material components: 50 parts of Proteus morgii ATCC 25829 culture, 50 parts of Proteus mirabilis ATCC 29906 culture, 5 parts of Streptomyces filamentous ATCC 31568 culture, 5 parts of Bacillus thuringiensis ATCC 39756 culture and Candida 10 copies of ATCC 204276 culture.

[0025] Preparation and application: (1) Inoculate each bacterial strain in the raw material components on a slant medium, respectively expand culture through shake flasks or solid medium to obtain cultures of various strains; wherein, shake flask culture Conditions are: temperature 30 DEG C, rotating speed 180rpm, culture 3 days, pH is 7.0; The substratum that each bacterial classification slant inoculates in the raw material component is respectively: Proteus morgii inoculation medium: Gao Shi No. 1 medium (soluble starch 20g / L, sodium chloride 0.5g / L, potassium nitrate 1.0g / L, potassium dihydrogen phosphate trihydrate 0.5g / L, magnesium sulfate heptahydrate 0.75g / L, magnesium sulfate heptahydrat...

Embodiment 3

[0028] Raw material components: 45 parts of Proteus morgii ATCC 23548 culture, 45 parts of Proteus mirabilis ATCC 12453 culture, 4 parts of Streptomyces filamentous ATCC 31568 culture, 3 parts of Bacillus thuringiensis ATCC 39756 culture and Candida 7 copies of ATCC 204276 culture.

[0029] Preparation and application: (1) Inoculate each bacterial strain in the raw material components on a slant medium, respectively expand culture through shake flasks or solid medium to obtain cultures of various strains, wherein the shake flask culture Conditions are: temperature 28 DEG C, rotating speed 170rpm, culture 2 days, pH is 6.8; The culture medium that each bacterial strain slant inoculates in the raw material component is respectively: Proteus mollerii inoculation medium: Gaoshi No. 1 medium (soluble starch 20g / L, sodium chloride 0.5g / L, potassium nitrate 1.0g / L, potassium dihydrogen phosphate trihydrate 0.5g / L, magnesium sulfate heptahydrate 0.75g / L, magnesium sulfate heptahydrate f...

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Abstract

The invention discloses a compound bacteria culture composition, which includes proteus morganii culture, proteus mirabilis culture, filamentous Streptomyces culture, bacillus thuringiensis culture and candida mycoderma bacteria culture. The compound bacteria culture composition is prepared by mixed-culturing proteus morganii culture, proteus mirabilis culture, filamentous Streptomyces culture, bacillus thuringiensis culture and candida mycoderma bacteria culture according to part by weight; under the condition of tolerating toxicity of heavy metals, the composition releases antibiotic and canplay safe and effective purifying effect to sewage commonly polluted by pathogenic bacteria and heavy metals.

Description

technical field [0001] The invention relates to a microbial composition and its preparation method and application, in particular to a compound bacterial culture composition for treating sewage polluted by pathogenic bacteria and heavy metals and its preparation method and application. Background technique [0002] Pathogenic microorganisms in water mainly come from human and animal manure and domestic sewage pollution, which can be divided into three categories: bacteria, viruses and protozoa; there are various ways for pollutants to enter water sources, and pollutants contained in waste gas, especially Particulate matter, which settles to natural water sources under the action of gravity, solid and liquid waste in domestic wastewater enters the water body directly along with the sewage pipes; in addition to pollutants that may easily cause public safety hazards such as pathogenic microorganisms in sewage, industrial water separation wastewater Carry a large amount of heavy...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/16C02F3/34C12R1/01C12R1/465C12R1/07C12R1/72C02F101/20
CPCC02F3/34C02F3/347C02F2101/20C12N1/16C12N1/20
Inventor 谢悦波姚竣耀刘才群
Owner 江苏世邦生物工程科技有限公司
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