Multiplex PCR detection primer set, method and kit for avian pathogenic Escherichia coli etc.

Abstract

The invention provides a multiplex PCR primer set for avian pathogenic Escherichia coli, Pasteurella multocida, Proteus mirabilis, Pseudomonas aeruginosa, Salmonella and Staphylococcus aureus, and multiplex PCR comprising the primer set A detection kit and a multiplex PCR detection method using the primer set, the primer set includes: a primer pair PhoA‑F and PhoA‑R for specific amplification of the phoA gene of avian Escherichia coli; a specific amplification of the Pasteurella multocida KMT1 gene The primer pair KMT‑F and KMT‑R; the primer pair AtpD‑F and AtpD‑R of the specific amplification Proteus mirabilis AtpD gene; the primer pair PETA‑F and PETA‑R of the specificity expansion Pseudomonas aeruginosa PETA gene; Primer pair InvA‑F and InvA‑R for specific amplification of Salmonella InvA gene; primer pair NuC‑F and NuC‑R for specific amplification of Staphylococcus aureus nuc gene.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular to a multiple PCR primer set for detecting avian pathogenic Escherichia coli, Pasteurella multocida, Proteus mirabilis, Pseudomonas aeruginosa, Salmonella and Staphylococcus aureus, including The multiple PCR detection kit of the primer set and the multiple PCR detection method using the primer set. Background technique [0002] Avian pathogenic Escherichia coli (APEC), Pasteurella multocida, Proteus mirabilis, Pseudomonas aeruginosa, Salmonella and golden yellow Staphylococcus (Staphylococcus aureus) is the main pathogenic microorganism that endangers the poultry industry. [0003] After infecting poultry with avian pathogenic Escherichia coli, symptoms such as hepatomegaly, intestinal mucosal bleeding and ulcers, and pericardial inflammation appear. Extensive use will easily cause drug residues in poultry products, seriously affecting human health and causing public health problems....

AI Technical Summary

Problems solved by technology

PCR technology is widely used because of its strong specificity, high sensitivity, simple and fast operation, but these methods usually only detect one or a few pathogenic bacteria in each experiment

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