Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Proteus mirabilis phage and application thereof

A technology of Proteus mirabilis and bacteriophage, applied in the direction of phage, virus/phage, application, etc., can solve the problems of poor heat resistance, achieve the effects of preventing and controlling food pollution, high safety, and wide range of acid-base tolerance

Pending Publication Date: 2022-01-28
GUANGXI UNIV
View PDF3 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Proteus mirabilis has poor heat resistance and can be killed by continuous heating at 55°C for 1 hour, but it can also reproduce at a low temperature of 4-7°C

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Proteus mirabilis phage and application thereof
  • Proteus mirabilis phage and application thereof
  • Proteus mirabilis phage and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Sewage samples were collected from cattle farms in many cities and counties in Guangxi Zhuang Autonomous Region, and fecal swabs were collected from pig farms. After the sewage was left to settle naturally, the upper layer liquid was taken. After centrifugation at 5000r / min at 4°C for 10 minutes, the supernatant was taken, and repeated three times. Filter with a 0.45 μm sterile filter, and then filter with a 0.22 μm sterile filter, and store the sewage filtrate in a refrigerator at 4°C for later use; use SS solid medium to isolate Proteus mirabilis Pm05 from the stool sample, which is the host bacterial liquid, and freeze it in -80℃, standby.

[0038]Inoculate the isolated Proteus mirabilis from pigs into LB liquid medium, culture in a constant temperature shaker at 37°C for 6 hours, save the cultured bacterial solution for backup, and mix it with the above-mentioned sewage filtrate stored in a refrigerator at 4°C. Add an equal volume of 2×LB liquid medium, shake and cu...

Embodiment 2

[0043] Enrichment of Proteus mirabilis phage vB_PmiP_pPm05

[0044] Add 2 mL of the fresh host bacteria solution in Example 1 and the spare phage stock solution in Example 1 to 5 mL of LB liquid medium, put it into a constant temperature shaker at 37°C and 180r / min to cultivate only the culture medium is clear, 6h, Centrifuge at 4°C and 8000rpm for 5min, take the supernatant, filter with a 0.22μm sterile filter, and the filtrate is the phage lysate. Add DNaseI and RNase A to the phage lysate to a final concentration of 1 μg / ml, incubate at 37°C for 30 minutes, add NaCl with a final concentration of 1M and bathe in ice for 1 hour (that is, add sodium chloride to make the final concentration of sodium chloride in the mixed solution The concentration is 1M), centrifuged at 4°C and 8000rpm for 10min, took the supernatant and added PEG 8000 with a volume concentration of 10%, overnight at 4°C, then centrifuged at 4°C and 8000rpm for 10min, discarded the supernatant, inverted for 5m...

Embodiment 3

[0047] Transmission Electron Microscopy Observation of Proteus mirabilis Phage

[0048] The concentrated phage suspension obtained in Example 2 was observed under an electron microscope, and 10 μL of the concentrated phage liquid was absorbed on the copper grid for 10 minutes, and the phage liquid was blotted from the periphery of the copper grid with filter paper, and the surface of the carbon film of the copper grid was covered with 2% phosphotungstic acid. The solution (pH 6.8) was dyed on the droplet for 10 min, and the dye solution was blotted from the periphery of the copper grid with filter paper, and dried naturally; then the prepared sample copper grid was observed with a transmission electron microscope, and the results were as follows: figure 2 Shown: the head of the phage is flat oval, the length of the head is about 145nm, and the length of the tail is about 10nm. According to the regulations of the International Committee on Taxonomy of Viruses, the phage belongs...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Diameteraaaaaaaaaa
Login to View More

Abstract

The invention discloses a proteus mirabilis phage vB_PmiP_pPm05, and an application of the proteus mirabilis phage vB_PmiP_pPm05 in the inhibition or killing of proteus mirabilis in food. The proteus mirabilis phage vB_PmiP_pPm05 is high in amplification efficiency, can be massively amplified in a short time, is wide in temperature and acid-base tolerance range, can effectively prevent and control food pollution caused by proteus mirabilis, and does not influence the texture and flavor of food.

Description

technical field [0001] The invention relates to the technical field of Proteus mirabilis phage, in particular to a strain of Proteus mirabilis phage and its application. Background technique [0002] The Proteus genus includes Proteus vulgaris, Proteus mirabilis, Proteus morganii, Proteus leiji and Proteus amerus. Among them, Proteus vulgaris and Proteus mirabilis are closely related to clinical practice. Proteus mirabilis (P. mirabilis) is a facultative anaerobic Gram-negative putrefactive bacterium, without capsule and spores, with pili and Flagella have strong locomotion ability. Proteus mirabilis has poor heat resistance, and it can be killed by continuous heating at 55°C for 1 hour, but it can also reproduce at a low temperature of 4-7°C. Proteus mirabilis is widely distributed in nature, mainly in water sources, soil, respiratory tract, digestive tract and excrement of humans and animals, and plays an important role in the decomposition of organic matter. Human bein...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N7/00A61K35/76A61P31/04A23L3/3571A23B4/22C12R1/92
CPCC12N7/00A61K35/76A61P31/04A23L3/3571A23B4/22C12N2795/10221C12N2795/10232C12N2795/10231
Inventor 王晓晔葛强李珣陆泽宁马东鑫周雨晴李磊王乐平韩凯欧曹雅洁胡传活
Owner GUANGXI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com