Serratia marcescens biocontrol bacterium for efficiently inhibiting aspergillus flavus compounded aflatoxin and its application

A technology of Serratia marcescens and aflatoxin, applied in the field of microbiology, can solve the problem that Serratia marcescens inhibits Aspergillus aflatoxin, etc.

Active Publication Date: 2018-03-23
INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the existing research, there is no report that Serratia marcescens inhibits Aspergillus flavus

Method used

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  • Serratia marcescens biocontrol bacterium for efficiently inhibiting aspergillus flavus compounded aflatoxin and its application
  • Serratia marcescens biocontrol bacterium for efficiently inhibiting aspergillus flavus compounded aflatoxin and its application
  • Serratia marcescens biocontrol bacterium for efficiently inhibiting aspergillus flavus compounded aflatoxin and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] 1) Activate Serratia marcescens 3J4SM on LB plates, culture them in a 37°C incubator for 24 hours, pick a single colony of the activated Serratia marcescens with a pick, and transfer to a plate containing 15 mL of LB liquid In the Erlenmeyer flask of the culture medium, shake culture at 28°C, 200r·min-1 for 12h. Aspirate 1% of the culture solution and transfer it to a Erlenmeyer flask filled with 15mL LB liquid medium for culture, and culture with shaking at 28°C and 200r·min-1 for 12h to obtain the fermentation broth of the antagonistic strain.

[0018] 2) Serratia marcescens fermentation broth (final concentration of 1 × 107 CFU / mL) was cultured for 7 days and vigorously growing Aspergillus flavus suspension (final concentration of spores was 5.0 × 10 5 Spores mL-1) were cultured together in Sabouraud liquid medium at 28°C and 200 rpm for 5 days, and each treatment was repeated 3 times.

[0019] 3) Measure the content of aflatoxin B1 in its culture solution (Table 2)...

Embodiment 2

[0024] 1) Take Zhonghua No. 6 peanut grains from the peanut field in Hubei and grind them into powder, weigh 1g of peanut powder in a petri dish, and add 1ml of Aspergillus flavus spore liquid (5×10 5 cells / mL) and 1ml CCTCC M 2017328 bacterial fluid (1×107 CFU / mL), with Sabouraud medium instead of CCTCC M 2017328 bacterial fluid as the control;

[0025] 2) Incubate the inoculated peanut powder in an incubator at 28° C. for 9 days, add 15 mL of 70% methanol water, vortex and put it on a shaker for 30 minutes. Take 3mL supernatant, add 8mL ultrapure water and vortex centrifuge;

[0026] 3) Take 8 mL of the supernatant and use the immunoaffinity column-HPLC method to determine the content of aflatoxin B1 (Table 3), and there are 3 repetitions in the experiment.

[0027] Table 3 Control effect of biocontrol bacteria on peanut aflatoxin

[0028]

[0029] It can be seen from the experimental results that the inhibition rate of CCTCC M 2017328 strain on the toxin production of ...

Embodiment 3

[0031] 1) Take 10 grains of Luhua No. 8 peanuts from Anhui peanut field, coat the surface of the peanuts with Serratia marcescens 3J4SM fermentation liquid, and add 1ml of Aspergillus flavus spore liquid (5×10 5 Individual / mL), with Sabouraud medium instead of CCTCC M 2017328 fermentation broth as a control;

[0032] 2) Cultivate the inoculated peanut grains in an incubator at 28°C for 9 days, then grind the peanut grains into peanut powder, add 15mL of 70% methanol water, vortex and place on a shaker for 30min. Take 3 mL supernatant, add 8 mL ultrapure water and vortex centrifuge;

[0033] 3) Take 8 mL of the supernatant and use the immunoaffinity column-HPLC method to determine the content of aflatoxin B1 (Table 4), and there are 3 repetitions in the experiment.

[0034] Table 4 Control effect of biocontrol bacteria on peanut Aspergillus flavus

[0035]

[0036] From the experimental results, it can be seen that the inhibition rate of CCTCC M 2017328 strain on Aspergill...

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Abstract

The invention belongs to the field of a microorganism, and particularly relates to serratia marcescens biocontrol bacterium for efficiently inhibiting aspergillus flavus compounded aflatoxin and its application. The serratia marcescens biocontrol bacterium 3J4SM has been preserved in the China typical culture preservation center (CCTCC for short) on June 13, 2017; the preservation address is WuhanUniversity of Wuhan of China; the preservation number is CCTCC No. M2017328. The enterobacter cloacae biocontrol strain 3J4SM can be used for inhibiting aspergillus flavus compounded aflatoxin and preventing the aflatoxin pollution of grain crops.

Description

technical field [0001] The invention belongs to the field of microorganisms, and in particular relates to a biocontrol strain of Serratia marcescens which can efficiently inhibit the synthesis of aflatoxin by Aspergillus flavus and its application. Background technique [0002] Aspergillus flavus is a pathogenic fungus that can produce a class of strong carcinogenic and highly toxic mycotoxins - aflatoxins, including B, G and M groups, of which B1 is the most common and the most toxic. It can widely pollute peanuts, corn and other food crops, seriously threaten the health of people and livestock, and cause great economic losses. Therefore, it is urgent to strengthen the prevention and control of Aspergillus flavus and toxin pollution. [0003] At present, in the control of Aspergillus flavus, there are three control methods: physical, chemical and biological. However, chemical control is not only costly, but also easy to pollute the environment. While preventing and contro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23B7/16A23B7/155C12R1/43A01N63/20
CPCA23B7/155A23B7/16C12N1/20C12R2001/43C12N1/205A01N63/20A23V2002/00
Inventor 李培武丁小霞白艺珍王同张奇
Owner INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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