A strain of Lactobacillus johnsonii with high ferulic acid esterase production and its application

A technology of Lactobacillus johnsonii and ferulic acid esterase, applied in the direction of application, enzymes, bacteria, etc., can solve the problems of constraints and expensive ferulic acid esterase, so as to improve palatability, promote sustainable agricultural development, fast growing effect

Active Publication Date: 2020-01-21
INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the high price of ferulic acid esterase, its practical application is restricted.

Method used

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  • A strain of Lactobacillus johnsonii with high ferulic acid esterase production and its application
  • A strain of Lactobacillus johnsonii with high ferulic acid esterase production and its application
  • A strain of Lactobacillus johnsonii with high ferulic acid esterase production and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Embodiment 1, the isolation and identification of HCOM3 bacterial strain

[0049] 1. Isolation of HCOM3 strain

[0050]HCOM3 was isolated from the colon of 1.5-month-old healthy piglets. The specific separation method is as follows: take the contents of the colon of healthy piglets at the age of one and a half months after dissection, and divide it with normal saline according to 10 -1 to 10 -7 Carry out gradient dilution, select the appropriate gradient and spread it on 1L MRS medium (the formula of 1L MRS medium is beef extract 10g, casein peptone 10g, yeast extract 5g, glucose 20g, sodium acetate 5g, diamine hydrogen citrate 2g, Tween80 1 mL, K 2 HPO 4 2g, MgSO 4 ·7H 2 O 0.58g, MnSO 4 ·7H 2 (0.25g, agar powder 1.5%, distilled water to 1L, autoclaved) or improved MC medium (1L improved MC medium formula is soybean peptone 5g, beef extract powder 3g, yeast extract powder 3g, Glucose 20g, lactose 20g, calcium carbonate 10g, agar powder 1.5%, distilled water to ...

Embodiment 2

[0055] Example 2, the detection of the acid production rate of the HCOM3 bacterial strain

[0056] The HCOM3 strain isolated and purified in Example 1 was inoculated into the MRS liquid medium, and fermented and cultured in a 37°C incubator for 4h, 16h, 28h, 40h or 52h, and samples were taken at each time period to detect the pH value of the fermentation broth. The fermentation time is plotted on the abscissa, and the pH value of the fermented liquid is plotted on the ordinate to draw a change curve.

[0057] The result is as figure 1 as shown ( figure 1 It is the curve graph of the HCOM3 strain cultured in MRS medium at 37°C for 52h). It can be seen from the figure that the HCOM3 bacterial strain of the present invention produces fast acid, grows fast, and has good growth performance.

Embodiment 3

[0058] Embodiment 3, the preparation of microbial preparation HCOM3

[0059] 1. Inoculate the HCOM3 strain isolated and purified in Example 1 into MRS liquid medium, and ferment and cultivate in a 37°C incubator for 24 hours to obtain HCOM3 fermentation broth;

[0060] 2. The HCOM3 fermentation broth was centrifuged at 10000-12000rpm for 2-5min, and the supernatant was collected to obtain the HCOM3 fermentation broth supernatant (the HCOM3 bacterial strain concentration was 10 14 -10 18 CFU / L). The HCOM3 fermentation broth supernatant was named microbial preparation HCOM3.

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Abstract

The invention discloses a strain of Lactobacillus johnsonii with high ferulic acid esterase production and application thereof. The lactobacillus provided by the present invention is Lactobacillus johnsonii HCOM3 CGMCC No.15447. Proved by experiments: Lactobacillus johnsonii HCOM3 CGMCC No.15447 of the present invention can secrete ferulic esterase, has the function of degrading the dense network structure of plant cell wall, and can be used to improve the cellulose digestibility in the high-fiber silage raw material, Improve the palatability of silage raw materials, increase the utilization rate of silage raw materials and improve the growth performance of animals. At the same time, it also has the characteristics of fast acid production, fast growth, non-toxic to humans and animals, and no pollution to the environment. It not only improves the quality of agricultural and animal husbandry products and protects the environment. It is of great significance and also helps to promote the sustainable development of agriculture.

Description

technical field [0001] The invention relates to a strain of Lactobacillus johnsonii derived from the gut of piglets, which can produce ferulic acid esterase at a high rate, has the function of degrading the dense network structure of plant cell walls, and is applied to the deep processing of high-fiber forage such as silage and yellow silage. Background technique [0002] As a large agricultural country, our country is extremely rich in straw resources, and the annual output of wheat alone can reach 115.4 million tons. However, about 50% of this abundant crop by-products are directly discarded in the field or burned, causing serious resource waste and environmental pollution problems. Faced with such a huge amount of straw, how to effectively and rationally use it is an urgent problem to be solved. There are many methods for comprehensive utilization of straw, among which biological treatment is not only environmentally friendly, but also effectively alleviates the shortage...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N9/18A23K30/18C12R1/225
CPCA23K30/18C12N9/18C12Y301/01073C12N1/205C12R2001/225
Inventor 钟瑾倪奎奎骆爱群苏日娜王天威
Owner INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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