Aspergillus oryzae for producing feruloyl esterase and application thereof

A technology of ferulic acid esterase and Aspergillus oryzae, applied in the biological field, can solve problems such as unfavorable sustainable development, and achieve the effects of easy cultivation and preservation, strong growth ability and rapid reproduction

Inactive Publication Date: 2014-10-08
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Although the chemical method is simple to operate and has high efficiency, the subsequent treatment of the extraction waste liquid is not conducive to sustainable development

Method used

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  • Aspergillus oryzae for producing feruloyl esterase and application thereof
  • Aspergillus oryzae for producing feruloyl esterase and application thereof
  • Aspergillus oryzae for producing feruloyl esterase and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Preliminary screening of Aspergillus oryzae, high-yielding strains were screened by the Oxford cup transparent circle screening experiment.

[0039] Soil samples were collected from the soil with a lot of saprophytic plants around South China University of Technology, and 10g of the sample was weighed and put into a 300mL triangular flask filled with 90mL of sterile saline containing glass beads and shaken for 20 minutes to make a 10-fold incremental serial dilution sample Homogenous solution 1:10, 1:100, 1:1000, and 1:10000, take 1.0mL each of the diluted sample homogeneous solution 1:100, 1:1000, and 1:10000 and add it to a sterile plate; add 15mL PDA for culture Mix the medium evenly, let it stand for 1min, and culture it upside down at 28°C for 72h, transfer the single colony on the medium to the slant PDA medium and culture it at 28°C for 72h, and then transfer to the primary screening medium containing ethyl ferulic acid 28 Cultivate at ℃ for 60 hours, screen the ...

Embodiment 2

[0042] Microbiological characteristics and identification of strains

[0043] Aspergillus oryzae ( Aspergillus oryzae ) rice 3 grows slowly on Cha's culture medium, with a diameter of 50mm after 7 days of culture; there are radial irregular grooves, velvet-like texture and flocculent in the center, many conidia structures, no exudate, and the reverse side is Pale pink to light brown. The colony grows fast on malt juice agar, and after 7 days of culture, it covers the entire dish, and the texture is velvety to thick flocculent; the yellow-green color is close to yellow olive, and then becomes light brown olive, and the reverse side is colorless. The colony was cultured on Saccharomyces chagheri paste agar for 7 days, with a diameter of 70mm, radial grooves, velvety or flocculent texture; many conidia structures, light yellowish brown.

[0044] Under the microscope, it can be observed that the conidia heads are spheroid and then radial. The conidiophores occur in the matrix or...

Embodiment 3

[0047] Determination of the Release Amount of Bound Ferulic Acid by Solid State Fermentation

[0048] According to the formula of seed culture medium, weigh 10g of bran and add 10g of water and mix it into a 500mL triangular flask. After the seed culture medium is sterilized, inoculate Aspergillus oryzae ( Aspergillus oryzae ) Mi 3 slant strain, the inoculation amount was 0.5%, and after culturing at 28°C for 3 days, the seeds of Aspergillus oryzae were obtained;

[0049] According to the formula of the fermentation medium, add 200g of bran to 200g of water, mix and sterilize, put it on a porcelain plate to cool, control the thickness of wheat bran within 2.5cm, and the inoculation amount of Aspergillus oryzae seeds is 0.5% (the bacterial content is 1~2×10 9 Individual / g), culture temperature is 28 ℃, carry out ventilated culture, fermentation time is 48h. Solid-state fermented bran was leached in a water bath at 40°C for 60 min at the ratio of 15 g to 90 mL of water, centrif...

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Abstract

The invention provides a strain of Aspergillus oryzae for producing feruloyl esterase and application thereof. The Aspergillus oryzae has the category name of Aspergillus oryzae M3, is collected by China center for type culture collection, called CCTCC for short, at December 8, 2013 and has the collection number of CCTCC NO: M2013640. According to the strain provided by the invention, a method that feruloyl esterase is produced through fermenting by taking wheat bran as a fermentation substrate and bound-type phenolic and ferulic acids are obtained through rupturing ester bonds between the polysaccharide and lignin of plant cell walls and ferulic acid by the feruloyl esterase is adopted, and the condition that microorganisms are short in growth cycle, are not restricted to climate and are easily subjected to large-scale production is utilized, so that a novel biological method for extracting bound-type phenolic acid substances, such as ferulic acid is provided.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a strain of Aspergillus oryzae producing ferulic acid esterase and its application. Background technique [0002] Wheat is one of the crops with the most extensive planting area in the world, accounting for about 26% of the planting area of ​​grain crops. The annual output of wheat in my country is high, and the annual output of wheat bran can reach more than 20 million tons. However, the development of bran has always been Utilization is seldom, and it is basically only used as feed raw materials. Wheat bran mainly contains non-starch polysaccharides, starch, fat, lignin, protein, vitamins and phenolic substances, among which arabinoxylan-based non-starch polysaccharides contain 46%, which is the main component of wheat cell walls. Element. Bran also contains a certain amount of phenolic acids, such as ferulic acid, p-coumaric acid, caffeic acid, sinapic acid and syring...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12N9/18C12R1/69
Inventor 吴晖胡博涵赖富饶刘素纯曾岚
Owner SOUTH CHINA UNIV OF TECH
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