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Aspergillus awamori for producing feruloyl esterase and application thereof

A technology of ferulic acid esterase and Aspergillus awamori, applied in the field of Aspergillus awamori, can solve problems such as unfavorable sustainable development, and achieve the effects of easy culture and preservation, strong growth ability and rapid reproduction

Inactive Publication Date: 2014-10-08
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Although the chemical method is simple to operate and has high efficiency, the subsequent treatment of the extraction waste liquid is not conducive to sustainable development

Method used

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  • Aspergillus awamori for producing feruloyl esterase and application thereof
  • Aspergillus awamori for producing feruloyl esterase and application thereof
  • Aspergillus awamori for producing feruloyl esterase and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0044] Preliminary screening of Aspergillus awamori, high-yielding strains were screened by the Oxford cup transparent circle screening experiment.

[0045] Collect samples from rotten orange peel, weigh 10g of the sample and put it into a 300mL Erlenmeyer flask filled with 90mL of sterile normal saline containing glass beads for 20 minutes, and make a 10-fold incremental serial dilution of the sample homogeneous solution 1:10, 1: 100, 1:1000, and 1:10000, take 1.0mL each of the diluted sample homogeneous solution 1:100, 1:1000, and 1:10000 and add it to a sterile plate; add 15mL of PDA medium to mix evenly, and let it stand for 1min. Cultivate upside down at 28°C for 72 hours, transfer the single colony on the culture medium to the slant PDA medium and culture it for 72 hours at 28°C, then transfer to the primary screening medium containing ethyl ferulic acid and cultivate it at 28°C for 60 hours, and screen for producing Ferulicum Ester strains, spare;

[0046] Use the flat...

Embodiment 2

[0048] Microbiological characteristics and identification of strains

[0049] Aspergillus awamori ( Aspergillus awamori ) Heiju antibacterial colonies grow slowly on Cha's medium, and the diameter is 30mm after 7 days of culture; the texture is velvety to powdery, the mycelium is white, the conidia structure is dark blue-green, and there is no exudate. The growth rate of colonies on malt juice agar is relatively fast, and the diameter is 45mm after 7 days of cultivation. The colony was cultured on Saccharomyces chagheri paste agar for 7 days, with a diameter of 40 mm, velvety texture, and a slightly thick bottle; conidia had many structures, grayish blue, thinner, white edges, and no exudate.

[0050] Under the microscope, it can be observed that the conidia heads are radial when they are young, with a diameter of 70-80 μm; the conidiophores occur in the stroma, the part near the apical sac is slightly contracted, the wall is smooth, and the upper part is yellowish brown; the...

Embodiment 3

[0053] Determination of the Release Amount of Bound Ferulic Acid by Solid State Fermentation

[0054] According to the seed medium formula, weigh 10g of bran and add 10g of water and mix it into a 500mL triangular flask. After the seed medium is sterilized, inoculate Aspergillus awamori ( Aspergillus awamori ) black orange bacteriostatic slant strain, the inoculum amount is 0.5%, and after culturing at 28°C for 3 days, the seeds of Aspergillus awamori are obtained;

[0055] According to the formula of the fermentation medium, add 200g of bran and 200g of water, mix and sterilize, put it on a porcelain plate to cool, control the thickness of wheat bran within 2.5cm, and the inoculum amount of Aspergillus awamori seeds is 0.5% (the bacterial content is 1~2×10 9 Individual / g), culture temperature is 28 ℃, carry out ventilated culture, fermentation time is 48h. Solid-state fermented bran was leached in a water bath at 40°C for 60 min at the ratio of 15 g to 90 mL of water, centri...

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Abstract

The invention provides Aspergillus awamori for producing feruloyl esterase and application thereof. The Aspergillus awamori has the category name of Aspergillus awamori black orange inhibiting strain, is collected by China center for type culture collection, called CCTCC for short, at December 8, 2013, has the collection number of CCTCCNO: M2013639 and is applied to the production of feruloyl esterase. According to the strain provided by the invention, a method that feruloyl esterase is produced through fermenting by taking wheat bran as a fermentation substrate and bound-type phenolic and ferulic acids are obtained through rupturing ester bonds between the polysaccharide and lignin of plant cell walls and ferulic acid by the feruloyl esterase is adopted, and the condition that microorganisms are short in growth cycle, are not restricted to climate and are easily subjected to large-scale production is utilized, so that a novel biological method for extracting bound-type phenolic acid substances, such as ferulic acid is provided.

Description

technical field [0001] The invention relates to a strain producing ferulic acid esterase and application thereof, in particular to a strain of Aspergillus awamori producing ferulic acid esterase and application thereof. [0002] Background technique [0003] Wheat is one of the crops with the most extensive planting area in the world, accounting for about 26% of the planting area of ​​grain crops. The annual output of wheat in my country is high, and the annual output of wheat bran can reach more than 20 million tons. However, the development of bran has always been Utilization is seldom, and it is basically only used as feed raw materials. Wheat bran mainly contains non-starch polysaccharides, starch, fat, lignin, protein, vitamins and phenolic substances, among which arabinoxylan-based non-starch polysaccharides contain 46%, which is the main component of wheat cell walls. Element. Bran also contains a certain amount of phenolic acids, such as ferulic acid, p-coumaric ac...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12N9/18C12Q1/44C12R1/665
Inventor 吴晖胡博涵赖富饶刘素纯曾岚
Owner SOUTH CHINA UNIV OF TECH
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