Aspergillus awamori for producing feruloyl esterase and application thereof
A technology of ferulic acid esterase and Aspergillus awamori, applied in the field of Aspergillus awamori, can solve problems such as unfavorable sustainable development, and achieve the effects of easy culture and preservation, strong growth ability and rapid reproduction
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Embodiment 1
[0044] Preliminary screening of Aspergillus awamori, high-yielding strains were screened by the Oxford cup transparent circle screening experiment.
[0045] Collect samples from rotten orange peel, weigh 10g of the sample and put it into a 300mL Erlenmeyer flask filled with 90mL of sterile normal saline containing glass beads for 20 minutes, and make a 10-fold incremental serial dilution of the sample homogeneous solution 1:10, 1: 100, 1:1000, and 1:10000, take 1.0mL each of the diluted sample homogeneous solution 1:100, 1:1000, and 1:10000 and add it to a sterile plate; add 15mL of PDA medium to mix evenly, and let it stand for 1min. Cultivate upside down at 28°C for 72 hours, transfer the single colony on the culture medium to the slant PDA medium and culture it for 72 hours at 28°C, then transfer to the primary screening medium containing ethyl ferulic acid and cultivate it at 28°C for 60 hours, and screen for producing Ferulicum Ester strains, spare;
[0046] Use the flat...
Embodiment 2
[0048] Microbiological characteristics and identification of strains
[0049] Aspergillus awamori ( Aspergillus awamori ) Heiju antibacterial colonies grow slowly on Cha's medium, and the diameter is 30mm after 7 days of culture; the texture is velvety to powdery, the mycelium is white, the conidia structure is dark blue-green, and there is no exudate. The growth rate of colonies on malt juice agar is relatively fast, and the diameter is 45mm after 7 days of cultivation. The colony was cultured on Saccharomyces chagheri paste agar for 7 days, with a diameter of 40 mm, velvety texture, and a slightly thick bottle; conidia had many structures, grayish blue, thinner, white edges, and no exudate.
[0050] Under the microscope, it can be observed that the conidia heads are radial when they are young, with a diameter of 70-80 μm; the conidiophores occur in the stroma, the part near the apical sac is slightly contracted, the wall is smooth, and the upper part is yellowish brown; the...
Embodiment 3
[0053] Determination of the Release Amount of Bound Ferulic Acid by Solid State Fermentation
[0054] According to the seed medium formula, weigh 10g of bran and add 10g of water and mix it into a 500mL triangular flask. After the seed medium is sterilized, inoculate Aspergillus awamori ( Aspergillus awamori ) black orange bacteriostatic slant strain, the inoculum amount is 0.5%, and after culturing at 28°C for 3 days, the seeds of Aspergillus awamori are obtained;
[0055] According to the formula of the fermentation medium, add 200g of bran and 200g of water, mix and sterilize, put it on a porcelain plate to cool, control the thickness of wheat bran within 2.5cm, and the inoculum amount of Aspergillus awamori seeds is 0.5% (the bacterial content is 1~2×10 9 Individual / g), culture temperature is 28 ℃, carry out ventilated culture, fermentation time is 48h. Solid-state fermented bran was leached in a water bath at 40°C for 60 min at the ratio of 15 g to 90 mL of water, centri...
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