71 results about "Aspergillus awamori" patented technology

The invention relates to Agrobacterium mediated transformation of moulds comprising species of the fungal sub-divisions Ascomycotina, Basidiomycotina, Deuteromycotina, Mastigomycotina, and Zygomycotina.Examples demonstrate the transformation of Aspergillus awamori (both protoplasts and conidia), Aspergillus nidulans, Aspergillus niger, Colletotrichum gloeosporioides, Fusarium solani pisi, Neurospora crassa, Trichoderma reesei, Pleurotus ostreatus and Agaricus bisporus (all conidia), and Fusarium graminearum (both conidia and rehydrated freeze dried ATCC material).Especially for Aspergillus awamori the transformation frequency is much higher than with conventional mould transformation techniques.It has further been found that not only one expressable gene can be introduced into these moulds, but even multiple copies of such gene, which, moreover, can be targeted e.g. in the chromosomal pyrG locus, as exemplified for A. awamori. These multiple copies can be of a gene encoding a desired, homologous or heterologous, protein.

The invention discloses a production method of a biological compound enzyme, belongs to the technical field of biology, and particularly relates to a biological compound enzyme product. The production method of the product comprises the following specific steps: respectively culturing and preparing aspergillus awamori culture, lactobacillus plantarum agent and bacillus subtilis inoculant, whereinthe inoculant is compounded and mixed in the following proportion: 50 to 80 parts of the aspergillus awamori culture, 5 to 10 parts of the lactobacillus plantarum agent, and 20 to 40 parts of the bacillus subtilis inoculant. Years of experiments prove that: the soil biological enzyme can promote soil loosening of an active soil layer, and improve soil fertility so as to build an excellent soil environment suitable for crop root growth; transformation of the soil biological enzyme plays remarkable effects in reducing agricultural production cost, reducing fertilizer use, recovering soil ecological land fertility, effectively increasing crop yield and the like; therefore, the efficacy of organic matters in soil can be sufficiently exerted.

The invention discloses a biological composite enzyme, belongs to the field of biotechnology and particularly relates to a biological composite enzyme product. The product provided by the invention consists of product of culture of aspergillus awamori, lactobacillus plantarum preparation and bacillus subtilis preparation. Each gram of biological composite enzyme contains 4.0 to 9.5*10<8> bacillus subtilis, 4.0 to 8.5*10<7> aspergillus awamori, 150 to 200IU of protease, 200 to 250IU of beta-glucanase, 50 to 150 IU of xylanase, 100 to 120 IU of cellulose and 250 to 300 IU of amylase. When the biological composite enzyme is applied into soil, the soil looseness of an active layer can be promoted, the soil fertility can be improved, and the sound soil environment suitable for the roots of crops to grow. The conversion of the soil bio-enzyme has obvious effects in aspects of reducing agricultural production cost, reducing consumption of fertilizer, restoring ecological productivity of soil, effectively improving crop yield, and the like and can give full play to the efficacy of the organic matters of the soil.

The invention discloses an organic bio-bacterial manure and a preparation method thereof. The organic bio-bacterial manure comprises the following raw materials in parts by weight: 15-20 parts of a lactobacillus plantarum agent, 1-2 parts of a phosphorus releasing bacteria agent, 2-4 parts of a potassium-releasing bacteria agent, 10-15 parts of a bacillus subtilis culture, 8-15 parts of an aspergillus awamori culture, 5-8 parts of polyglutamic acid, 50-100 parts of humic acid, 100-150 parts of organic fertilizers, 80-100 parts of chemical fertilizers and 50-80 parts of bentonite. According to a formula, the organic bio-bacterial manure disclosed by the invention contains the organic fertilizers, is capable of increasing the utilization rate of the chemical fertilizers, improving soil hardening, effectively supplementing organic matters of soil and improving the disease-resistant capacity of crops, and particularly is good in the effect of preventing and treating soil-borne diseases and the continuous cropping disease of the crops due to compound micro-organisms.

The invention discloses a masson pine rhizosphere solubilizing phosphorus fungus aspergillus awamori which is aspergillus awamori JP-NJ1 and application thereof. The aspergillus awamori JP-NJ1 is collected in the China Center for Type Culture Collection (CCTCC) on May 14, 2012 with the collection number of CCTCC No: M2012166. The aspergillus awamori JP-NJ1 disclosed by the invention has strong capacity of dissolving the insoluble phosphate which is the calcium phosphate. The room-temperature pot culture experiments prove that the aspergillus awamori JP-NJ1 can promote the growth of the massonpine seedlings significantly. Therefore, the aspergillus awamori provides excellent strain resources for the development of the masson pine growth promoting bacteria and has good application and development prospects.

The invention discloses an efficient micro-ecological bio-fertilizer and its preparation method. The invention relates to a micro-ecological fertilizer and belongs to the technical field of agricultural fertilizers. The efficient micro-ecological bio-fertilizer comprises the following ingredients, by weight, 13-26 parts of lactobacillus plantarum, 10-16 parts of aspergillus niger culture, 1-5 parts of bacillus mucilaginosus microbial inoculum, 1-3 parts of phosphate-solubilizing bacillus megaterium microbial inoculum, 8-15 parts of bacillus subtilis powder, 2-6 parts of aspergillus awamori culture and 1-3 parts of polyglutamic acid. polyglutamic acid on the surface of the fertilizer particles can absorb moisture during the actual use process so as to form hydrogel. Thus, a moisture environment is provided for rapid growth and propagation of the granule interior fertilizer. Meanwhile, the effect of slowly releasing fertility of the bio-fertilizer also can be achieved, and irrigation water capacity in the growth stage of crops can effectively be reduced by more than 13%.

The invention discloses a method for producing methane through fungal solid-state fermentation of kitchen waste. The method comprises the following steps: sorting a certain amount of kitchen waste forimpurity removal, and then mechanically crushing to form kitchen waste to be treated; selecting 5-20% of the kitchen waste to be treated, and adding fungal aspergillus awamori and aspergillus oryzaefor solid-state fermentation to culture a solid substance rich in amylase and proteinase; adding the cultured solid substance rich in the amylase and the proteinase into water-mixed kitchen waste forhydrolyzing to form a hydrolyzate rich in small molecular nutrient substances (glucose and amino acids); successively performing solid-liquid separation and oil-water separation on the hydrolyzate toprepare a kitchen waste hydrolyzate; adding the kitchen waste hydrolyzate into an anaerobic fermentation tank for producing the methane. According to the method, the amylase and the proteinase are cultured through the fungal solid-state fermentation to hydrolyze the kitchen waste, so that the hydrolysis and liquefaction speed of the kitchen waste can be increased, the utilization rate of a substrate is increased, and further the yield of the methane is increased.

The invention discloses a method for producing cellulose and polypeptide protein feed by fermenting with Aspergillus awamori 1042-2 which belongs to the Aspergillus and has the collection registration number of M209142 in the China Center for Type Culture Collection (CCTCC). The method for producing the cellulose and the polypeptide protein feed comprises the following steps: A, selecting the Aspergillus awamori 1042-2; B, authenticating the Aspergillus awamori 1042-2, C, culturing the Aspergillus awamori 1042-2 liquid; D, fermenting the solid material; and F, postprocessing. The solid culture which is obtained by inoculating the Aspergillus awamori 1042-2 onto the solid culture medium and fermenting the Aspergillus awamori 1042-2 and the solid culture medium is baked to dry, ground, sieved and subjected to the detection of the enzyme activity and the polypeptide protein content. The method for producing the cellulose and the polypeptide protein feed is feasible and easy, the produced powdered cellulose has the CMC enzyme activity of 31397.1-41826.7 U/g and the FPA enzyme activity of 1779.3-2212.1 U/g, has good stability and can be produced on a large scale, and the produced polypeptide protein feed contains more than 35 percent of protein. The Aspergillus awamori 1042-2 can be used for fermenting the palm kernel dregs, other dregs and the vegetable fiber and producing the biological feed, the biological fertilizer, the biological fuel and can be also used in the food industry, chemical industry and the pharmaceutical industry. The polypeptide protein feed produced by fermenting with the Aspergillus awamori 1042-2 can be used as the additive of the protein of various feed.

The invention discloses a high-efficiency microbial fertilizer, belonging to the technical field of agricultural fertilizers. The high-efficiency microbial fertilizer is composed of 2-5 parts of Bacillus mucilaginosus, 1-3 parts of phosphate solubilizing bacteria, 20-35 parts of Bacillus subtilis culture, 6-8 parts of Aspergillus awamori culture and 10-15 parts of Lactobacillus plantarum inoculum. The fertilizer can improve the soil; the beneficial microbes in the fertilizer can generate saccharides and plant mucilage; the mineral embryo can be combined with the organic colloids to improve the crumb structure of the soil, enhance the physical properties of the soil and reduce the loss of the soil particles; and the fertilizer can participate in humification under certain conditions, thereby being beneficial to increasing the soil fertility. The product is simple in application method, the consumption is 0.3-1kg per mou, the cost is only 70-100 Yuan/mou, and the product is sprayed on the field surface before irrigation in the seed treatment or growth period.

The invention discloses feed additive and a production method thereof. The invention belongs to the field of feed additive, especially relates to the product of cow feed additive. The feed comprises the following components by weight in percent: 30-50 wt% of extruded soybean, 4-9 wt% of urea, 3-8 wt% of yeast protein, 20-30 wt% of gloeostereum insarnatum, 15-25 wt% of ganoderma lucidum, 2-9 wt% of slow-releasing agent, and 3-6 wt% of aspergillus awamori culture. The feed production method provided by the invention provides fully nutrient storage for the cows at the perinatal period, improves the daily milk production and peak sustainable power, and improves the pregnancy rate of the cows.

The invention discloses a green microecological compound fertilizer which is composed of an Aspergillus niger nutrient material, a Bacillus mucilaginosus microbial inoculum, a phosphate-solubilizing Bacillus megaterium microbial inoculum, a Bacillus subtilis nutrient material, an Aspergillus awamori nutrient material and a lactobacillus plantarum microbial inoculum. The fertilizer has the advantages of abundant nutrition and high organic content. The fertilizer can satisfy the production demands for organic food. The fertilizer is prepared by fermenting compound microbes, can improve the microecological environment of soil, and provides beneficial conditions for crop growth.

The invention discloses a biofertilizer and a preparation method thereof, belonging to the technical field of agricultural fertilizers. The biofertilizer comprises the following components in parts by weight: 20-35 parts of plant lactobacillus, 1-2 parts of phosphate solubilizing bacteria, 2-4 parts of potassium solubilizing bacteria, 10-15 parts of bacillus subtilis culture, 8-15 parts of aspergillus awamori culture and 5-8 parts of polyglutamic acid. The preparation method comprises the following steps: uniformly mixing plant lactobacillus, the phosphate solubilizing bacteria, the potassium solubilizing bacteria, the bacillus subtilis culture and the aspergillus awamori culture in proportion; putting the mixture into a granulator, granulating, controlling the grain sizes between 0.5mm and 2mm, then enabling the fertilizer granules to adhere to the added humid polyglutamic acid, then controlling the temperature at 30-40 DEG C and maintaining the temperature for 1-3 hours. The fertilizer can be used for enhancing the drought resistance capabilities of crops, improving the physical properties of soil, improving the use efficiency, regulating acid-base balance of soil and reducing pollution of harmful substances in soil, such as nitrite and is conductive to increasing soil fertility.

The method relates to a method for preparing a sumac extract by adopting aspergillus fermentation of aspergillus oryzae and the like and the sumac extract prepared by the method, in particular to the method for preparing the sumac extract with the following steps: a) the sumac is dried, grinded and water is added to carry out moisture absorption on the sumac; b) hygroscopic material prepared in step a) is cooked; c) aspergillus is inoculated, nurtured and fermented in the cooked material prepared in step b), the aspergillus is selected from a group composed of aspergillus oryzae, aspergillus kawachii, aspergillus usamii, aspergillus shirousamii, aspergillus niger and aspergillus awamori; d) water is added to leavening prepared in step c) and is heated; e) liquid ingredients are recovered from the heated material prepared in step d). The method for preparing the sumac extract of the invention sharply improves efficiency of numerous active ingredients of the sumac, eliminates toxic ingredients thereof and improves functional characteristics of the prepared sumac extract.

The invention discloses an Aspergillus awamori 2-17 strain for preventing and treating fusarium wilt of cucumber, which is collected to Common Microorganism Center of China Committee for Culture Collection of Microorganisms positioned in Yard 1 No.3, Beichenxi Road, Chaoyang District, Beijing City on 11th January, 2013, wherein the collection number is CGMCC No.7127. The invention also discloses a microbial inoculant prepared from the strain and a preparation method thereof. The Aspergillus awamori 2-17 and microbial inoculant thereof have obvious preventing and treating effects on fusarium wilt of cucumber, can control the morbidity within 5%, can not easily generate drug resistance, are safe for human and livestock, can not pollute water and soil, are harmless to crops, and provide a new efficient microbial way for preventing and treating fusarium wilt of cucumber.

The invention relates to Aspergillus awamori, its microbe inocula and an application thereof. The invention discloses aspergillus awamori used for preventing and treating damping off at a seeding stage of tomato, the bacterial strain is preserved in the General Microbiological Center of China Committee of Culture Collection for Microorganisms, in No.3 yard, Bei Chenxi Road No. 1, Chaoyang District, Beijing, and the preservation number CGMCC No. 10520. The invention also discloses the microbe inocula produced by the bacterial strain and a preparation method. The provided Streptomyces pseudogriseolus nmg4 and its microbe inocula, antagonistic action is strong, incidence of damping off disease at the seeding stage of tomato is reduced, strain height and stem thickness of the tomato sprout are increased, tomato growth is promoted, the semi-fermentation decomposed mushroom slag and a vinasse matrix are compared, and rate of prevention and control of the matrix matrix prepared by the antagonistic bacteria co-culture mushroom slag is better.

The invention discloses a preparation method of a biological compound fertilizer, belonging to the technical field of agricultural fertilizers. The preparation method of the biological compound fertilizer comprises the following steps: mixing 5-18 parts by weight of Bacillus mucilaginosus, 2-6 parts by weight of phosphate-solubilizing bacterium, 5-10 parts by weight of potassium bacterium, 18-30 parts by weight of Bacillus subtilis nutrient material, 5-10 parts by weight of Aspergillus awamori nutrient material and 3-8 parts by weight of lactobacillus plantarum microbial inoculum, or mixing and granulating. The fertilizer can enhance the drought resistance of the crops; the Bacillus mucilaginosus in the compound strain is prepared by industrially fermenting high-potassium-solubilizing-capacity silicate bacterium screened from crop roots in a specific culture medium, and can secrete auxin substances, gibberellin substances and other growth promoting substances to reinforce the roots and seedlings; the silicon in the soil is decomposed and supplied to the plant, so that the waxy layer of the plant is thickened, thereby enhancing the water retention capacity of the plant; and the fertilizer can promote the formation of the soil aggregate from, keep soil from packing together, destroy the soil capillarity and prevent the soil moisture from evaporation.

The invention discloses a microbial bacterium, which is prepared from the following raw materials in parts by weight: mixed biological bacteria including 0.01 to 0.02 part of aspergillus foetidus, 0.02 to 0.04 part of aspergillus awamori, 0.01 to 0.02 part of bacillus mucilaginosus, 0.01 to 0.02 part of streptomyces roseoflavus, 0.01 to 0.02 part of bacillus benzoevorans, 0.03 to 0.05 part of bacillus megatherium and 0.03 to 0.05 part of bacillus polymyxa; other materials including 60 to 70 parts of sweet buckwheat, 10 to 12 parts of mangosteen peel powder, 3 to 5 parts of copper sulfate, 2 to 4 parts of ferric chloride, 5 to 7 parts of zinc chloride, 1 to 2 parts of yeast extracts, 0.3 to 0.6 part of phellodendron extracts and 1 to 2 parts of catechin. The microbial bacterium has the advantages that the matching is reasonable; the livestock and poultry manure can be effectively treated and decomposed.

The invention discloses a preparation method of a high-efficiency microbial fertilizer, belonging to the technical field of agricultural fertilizers. The high-efficiency microbial fertilizer is prepared by mixing or mixing and granulating the following components in parts by weight: 2-5 parts of Bacillus mucilaginosus, 1-3 parts of phosphate solubilizing bacteria, 20-35 parts of Bacillus subtilis culture, 6-8 parts of Aspergillus awamori culture and 10-15 parts of Lactobacillus plantarum inoculum. The fertilizer can improve the soil; the beneficial microbes in the fertilizer can generate saccharides and plant mucilage; the mineral embryo can be combined with the organic colloids to improve the crumb structure of the soil, enhance the physical properties of the soil and reduce the loss of the soil particles; and the fertilizer can participate in humification under certain conditions, thereby being beneficial to increasing the soil fertility. The product is simple in application method, the consumption is 0.3-1kg per mou, the cost is only 70-100 Yuan/mou, and the product is sprayed on the field surface before irrigation in the seed treatment or growth period.

The invention provides a composite microbial agent and application thereof. The composite microbial agent comprises an aspergillus awamori preparation and a bacillus siamensis preparation, wherein aspergillus awamori in the aspergillus awamori preparation is collected in China General Microbiological Culture Collection Center of China Committee for Culture Collection of Microorganisms on October,15, 2019 and has an accession number of CGMCC No. 18595, and bacillus siamensis in the bacillus siamensis preparation is collected in China General Microbiological Culture Collection Center of China Committee for Culture Collection of Microorganisms on October, 15, 2019 and has an accession number of CGMCC No. 18688. The composite microbial agent provided by the invention has relatively good cellulose decomposing capability and can be effectively applied to straw composting, a temperature rise speed of a primary stage of composting is obviously accelerated, a fermentation temperature is increased, the rate of weight loss of straws after composting is increased, the C/N content is lowered, the well-composted degree of composting is high, and thus, the composite microbial agent is applicableto large-scale application.

The invention specifically discloses a preparation method for a great burdock fruit extract through combined microbial fermentation, which belongs to the technical field of preparation of traditional Chinese medicinal material extracts through microbial fermentation. According to the invention, the great burdock fruit extract is prepared through combined fermentation of Aspergillus awamori var. CICC2203 and Trichoderma reesei CICC40932, optimization study is carried out on fermentation process conditions, so a conversion rate of arctiin in great burdock fruit into arctigenin is substantially improved and reaches 99.9%, the problem of a low conversion rate of arctiin into arctigenin is effectively overcome, and a technical problem of industrial production of the great burdock fruit extract is overcome.

The invention discloses a method for preparing arctigenin. The method comprises the following steps of activating strains, preparing seed microbial liquid, fermenting fructus arctii powder through trichoderma reesei and aspergillus awamori, conducting ultrasonic-wave-assisted extraction to obtain fructus arctii crude extract and conducting silica gel column chromatography purification. According to the method, the fructus arctii powder serves as the raw material to prepare the fructus arctii extract through a biological fermentation technology, the fructus arctii powder is processed through fermentation of beta-glucosidase and a cellulase high-producing strain, the glucosidic bond of burdock glycoside is ruptured to be converted into the arctigenin under the in-vitro controllable condition, the content of arctigenin in fructus arctii is increased, a simple and effective extraction method is adopted, and high-purity arctigenin can be extracted.

The invention discloses an allelochemicals-containing biological compound fertilizer. A production process of the allelochemicals-containing biological compound fertilizer comprises the following steps of: culturing and expanding aspergillus awamori, pseudomonas fluorescens, bacillvss ubtilis and geotrichum candidum serving as strains, and preparing the cultured and expanded strains in the ratio of 3:3:2:2 into a mixed strain; and mixing the mixed strain with other materials such as a humus-containing organic compound, boron urea, a cementing agent and the like in a weight ratio, and adding chinaberry seed powder and castor seed powder to obtain the allelochemicals-containing biological compound fertilizer. The allelochemicals-containing biological compound fertilizer prepared by the production process has the advantages of suppressing nitrification, remarkably increasing the utilization ratio of a nitrogen fertilizer, reducing the loss rate of urea by 50 percent, reducing the loss rate of an ammonium fertilizer or an ammonium-containing nitrogen fertilizer by 25 percent, activating soil nutrient, improving crop quality, promoting the growth of plants and increasing the yield of crops by 25.7 percent on average, along with certain rat-preventing and worm-killing functions, reasonable formula, simple process and stable quality. The fertilizer is a novel allelochemicals-containing biological compound fertilizer.