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Preparation method of green microecological compound fertilizer

A technology of micro-ecology and compound fertilizer, which is applied in the direction of organic fertilizers, can solve the problems of unstable product quality and application effect, the overall level of microbial fertilizer industry is not high, and lack of investment in research, so as to prevent soil moisture from evaporating and improve micro The effect of improving the ecological environment and improving soil fertility

Inactive Publication Date: 2014-04-23
HEBEI LVTIAN BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Due to the long-term lack of investment in microbial fertilizer research in China, my country's microbial fertilizer industry still has problems such as low overall level, insufficient technological innovation, and unstable product quality and application effects.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0042] (1) Preparation of bacterial suspension

[0043] Inoculate the Li-2013 single colony grown after streaking on the plate into the seed medium, culture at 40°C for 12 hours at 100 r / min, take 1 mL of the culture medium, centrifuge, wash twice with normal saline, and resuspend with 9 mL of physiological in salt water.

[0044] (2) Ultraviolet-Lithium Chloride-Diethyl Sulfate Compound Mutagenesis

[0045] The bacterial suspension was placed on a sterile plate, stirred and irradiated for 100s under a UV lamp with a distance of 30cm and a power of 15w. The irradiated bacteria solution was diluted and spread on the lithium chloride plate after gradient dilution, and the bacteria solution without ultraviolet irradiation was diluted and spread on the plate as a control. Wrap the uniformly coated plate above with black cloth or newspaper, incubate at 40°C for 48 hours, screen out the plate with the largest ratio of hydrolysis circle to colony diameter on the plate that grows co...

Embodiment 1

[0064] A kind of green micro-ecological compound fertilizer is provided: the number of parts by weight is as follows: 6 parts of Aspergillus niger culture, 5 parts of colloid bacillus bacteria agent, 5 parts of phosphorus-solubilizing bacillus megaterium bacteria agent, 10 parts of bacillus subtilis culture, awamori Aspergillus culture 6, Lactobacillus plantarum agent 3 parts.

[0065] The strains used are as follows:

[0066] Bacillus subtilis subsp CGMCC7926

[0067] Lactobacillus plantarum CICC20764

[0068] Aspergillus awamori (Aspergillus awamori) CGMCC3.6484

[0069] The preservation number of Aspergillus niger Li-2013-03 is CGMCC NO.7927.

[0070] The preparation method of Awamori Aspergillus agent:

[0071] The technical solution is as follows:

[0072] Activation culture of slant strains: transfer Aspergillus awamori slant strains to the slant medium, and culture at 27°C for 3 days.

[0073] Solid first-level seed culture: pick the Aspergillus awamori slant stra...

Embodiment 2

[0094] Basically the same as embodiment 1

[0095] A kind of green micro-ecological compound fertilizer is provided: the number of parts by weight is: 5 parts of Aspergillus niger culture, 7 parts of colloid bacillus bacteria agent, 6 parts of phosphorus-solubilizing bacillus megaterium bacteria agent, 12 parts of bacillus subtilis culture, awamori Aspergillus culture 6, plantarum lactobacillus agent 2 parts.

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Abstract

The invention discloses a preparation method of a green microecological compound fertilizer, belonging to the technical field of agricultural fertilizers. The preparation method of the green microecological compound fertilizer comprises the following step: proportionally combining an Aspergillus niger nutrient material, a Bacillus mucilaginosus microbial inoculum, a phosphate-solubilizing Bacillus megaterium microbial inoculum, a Bacillus subtilis nutrient material, an Aspergillus awamori nutrient material and a lactobacillus plantarum microbial inoculum to obtain the microecological compound fertilizer. The fertilizer is prepared by fermenting compound microbes, can improve the microecological environment of soil, and provides beneficial conditions for crop growth. The fertilizer can improve the soil. The beneficial microbes in the fertilizer can generate saccharides (accounting for 0.1% of soil organic matters), the saccharides can be combined with the plant mucilage, mineral embryo and organic colloid to improve the crumb structure of the soil, enhance the physical properties of the soil and reduce the loss of the soil particles; and the fertilizer can participate in humification and improve the physical behaviors of the soil under certain conditions, thereby being beneficial to increasing the soil fertility.

Description

technical field [0001] The invention relates to a preparation method of a green micro-ecological compound fertilizer, which belongs to the technical field of agricultural fertilizers. Background technique [0002] Microbial fertilizer is a microbial living product that uses microbial life activities and its products to cause crops to obtain specific fertilizer effects. It can improve soil fertility, improve fertilizer utilization, inhibit crops from absorbing harmful substances such as heavy metals and pesticides, and purify and restore soil. It plays an irreplaceable role in promoting the utilization of crop stalks and urban waste, and improving the quality of agricultural products. The efficacy of microbial fertilizers is mainly achieved through the synergistic effect on traditional chemical fertilizers and organic fertilizers, the improvement and activation of soil, and the physiological effects of microorganisms. Therefore, microbial fertilizers have a variety of fertil...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05F11/08
Inventor 邵素英孔日祥
Owner HEBEI LVTIAN BIOTECH
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