Aspergillus awamori and its microbe inocula and application thereof

A technology of Streptomyces pseudogreyishus and microbial agents, applied in the field of preventing and treating tomato seedling blight, can solve the problems of environmental pollution, economic loss of agricultural production, etc., increase stem diameter, reduce morbidity, prevent The effect of excellent control rate

Active Publication Date: 2015-09-16
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Yellow damping-off is a fungal disease caused by pathogenic Pythium spp. , peppers, cucumbers, kale, celery, onions, kidney beans; lettuce and other vegetable seedlings are common diseases in spring and autumn seedling cultivation in protected areas in China. The incidence rate in general nurseries is 20% to 50%, and it can reach more than 80% in severe cases At present, it is also more prominent in substrate seedling cultivation, which has caused huge economic losses to agricultural production.
At present, the prevention and control of damping-off disease in China is still dominated by chemical control, but it seriously pollutes the environment, and with the prolongation of the use of chemicals, it also induces the disease resistance of pathogenic bacteria. Therefore, the development of green damping-off prevention and control technology has been extensive attention

Method used

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  • Aspergillus awamori and its microbe inocula and application thereof
  • Aspergillus awamori and its microbe inocula and application thereof
  • Aspergillus awamori and its microbe inocula and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0030] Preparation of bacteria.

[0031] Explanation of the materials involved: The tomato variety tested is Zheza 205; the Streptomyces pseudogriseolus strain nmg4 (Streptomyces pseudogriseolus), which is an antagonistic strain to Streptomyces pseudogriseolus, was isolated and preserved in our laboratory. Damping-off pathogenic strain Pythium ultima ( Phthium ultinum , ACCC36075, hereinafter referred to as 36075) was purchased from China Agricultural Microorganism Culture Collection Center.

[0032] Streptomyces pseudogriseolus nmg4 adopts Actinomyces No. 1 Gaoshi culture medium, and PDA medium is used for the cultivation of the pathogenic bacteria of Streptomyces pseudogiseolus.

[0033] Substrate material and co-cultivation substrate: conventional substrate raw materials are respectively fermented and decomposed rice wine distiller's grains and mushroom residues after cultivation of Flammulina velutipes; semi-fermented and decomposed distiller's grains and mushroom resi...

Embodiment 2

[0037] Seedling comparison test.

[0038] Tomato seeds were selected for the antagonistic substrate seedling cultivation experiment. Three treatments were set up in the experiment: conventional substrate inoculated with pathogenic bacteria, antagonistic bacteria co-cultivated substrate, and antagonistic bacteria co-cultured substrate inoculated with pathogenic bacteria. A 25-hole seedling tray was used, and the experiment was repeated 3 times. Before sowing, mix the co-cultivation antagonistic matrix and conventional matrix materials, perlite and vermiculite in a ratio of 8:1:1, and then add compound fertilizer (15-15-15) and 2% of The 10-fold diluted pathogenic bacteria culture solution was mixed evenly with the substrate in proportion. Seeds were germinated in an incubator at 28°C, and sown in plug trays in time after germination. The seedlings were tested regularly, and the incidence rate was recorded. After the test, 10 plants were randomly selected for each repetition ...

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Abstract

The invention relates to Aspergillus awamori, its microbe inocula and an application thereof. The invention discloses aspergillus awamori used for preventing and treating damping off at a seeding stage of tomato, the bacterial strain is preserved in the General Microbiological Center of China Committee of Culture Collection for Microorganisms, in No.3 yard, Bei Chenxi Road No. 1, Chaoyang District, Beijing, and the preservation number CGMCC No. 10520. The invention also discloses the microbe inocula produced by the bacterial strain and a preparation method. The provided Streptomyces pseudogriseolus nmg4 and its microbe inocula, antagonistic action is strong, incidence of damping off disease at the seeding stage of tomato is reduced, strain height and stem thickness of the tomato sprout are increased, tomato growth is promoted, the semi-fermentation decomposed mushroom slag and a vinasse matrix are compared, and rate of prevention and control of the matrix matrix prepared by the antagonistic bacteria co-culture mushroom slag is better.

Description

technical field [0001] The present invention specifically relates to a Streptomyces pseudogreyiseus for preventing and treating tomato seedling blight and a microbial inoculant containing the same, and its application in preventing and treating tomato blight at seedling stage, which belongs to agriculture field of microbiology. Background technique [0002] Yellow damping-off is a fungal disease caused by pathogenic Pythium spp. It is a world-renowned and devastating disease that can damage eggplant, tomato, pepper, cucumber, kale, celery, onion, kidney bean; lettuce and many other vegetable seedlings are common diseases in spring and autumn seedling cultivation in China's protected areas. The incidence rate in general nurseries is 20% to 50%, and it can reach more than 80% in severe cases. Huge economic loss. At present, the prevention and control of damping-off disease in China is still dominated by chemical control, but it seriously pollutes the environment, and with th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01P3/00C12R1/465
CPCA01N63/00C12N1/20C12N1/205C12R2001/465
Inventor 王卫平洪春来姚燕来朱凤香陈晓旸薛智勇
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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