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Preparation method of biological compound fertilizer

A biological compound fertilizer and seed technology, applied in the direction of educts, organic fertilizers, reagents, etc., can solve the problems of lack of fermentation metabolites, single microbial strains, single function, etc., to improve soil aggregate structure, improve soil physical properties, The effect of improving water retention capacity

Active Publication Date: 2014-04-23
山东省神农生态科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] For many years, most of the processing methods of microbial fertilizers only use finished bacterial agents mixed with fillers, lacking fermentation metabolites, which seriously affect the fertilizer efficiency of microbial fertilizer products. For example, the patent application with publication number CN103396252A discloses a compound microorganism containing amino acids Fertilizer, in which the finished microbial agent is composed of Bacillus subtilis, Bacillus licheniformis and Bacillus megaterium mixed in a certain proportion
[0006] Moreover, the agricultural microbial agents currently on the market are mainly single microbial strains, which are difficult to restore the micro-ecological structure. At the same time, the synergy between common strains and crops is weak, which is unfavorable such as improving plant stress resistance and root nutrient absorption. For example, the publication number is The patent application of CN102888356A discloses a method and application of using Bacillus subtilis to prepare microbial fertilizer
Although some compound microbial agents have appeared on the market, their functions are single, or their use methods are limited, and the effect of increasing crop production after use is still unsatisfactory. Composite microbial agent or fertilizer with comprehensive functions and good yield-increasing effect
[0007] Due to the long-term lack of investment in microbial fertilizer research in China, my country's microbial fertilizer industry still has problems such as low overall level, insufficient technological innovation, and unstable product quality and application effects.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Bacillus subtilis (Bacillus subtilis) Li-2013-02, the strain has been preserved in the General Microorganism Center of China Committee for Culture Collection of Microorganisms (CGMCC for short) on July 15, 2013, the address is: Beichen West Road, Chaoyang District, Beijing, China No. 1 Courtyard No. 3), the preservation number is CGMCC No.7926.

[0030] The characteristic of the strain is that it has high enzyme activity for producing high temperature-resistant α-amylase, and strong heat resistance and acid resistance.

[0031] The high temperature-resistant α-amylase enzyme activity prepared by the strain is 30000-35000u / ml; the applicable temperature range is 105-115°C, the optimum reaction temperature is 110°C, and the enzyme activity is completely stable at 110°C; the applicable reaction pH range is 3.0-7.0, when the pH value is 3.0, the enzyme activity is completely stable, and the optimum reaction pH value is 4.2.

[0032] Described bacterial strain characteristi...

Embodiment 2

[0092] A biological compound fertilizer, the number of parts by weight is: 12 parts of colloidal bacillus, 5 parts of phosphorus solubilizing bacteria, 8 parts of potassium solubilizing bacteria, 25 parts of bacillus subtilis culture, 7 parts of aspergillus awamori culture, plant lactobacillus agent 6 servings.

[0093] Preparation method of Bacillus subtilis culture:

[0094] Obtaining of fermented liquid: Obtaining Bacillus subtilis fermented liquid by step-by-step expansion culture of slanted strains;

[0095] (1) Primary seed cultivation: Inoculate the slant strain of Bacillus subtilis into a 500-mL shaker flask with a medium capacity of 100 mL, a rotary shaker at 180 rpm, a cultivation temperature of 30°C, and a cultivation time of 24 hours;

[0096] (2) Secondary seed cultivation: Put the first-grade seeds into 500 ml second-grade seed shake flasks according to the inoculation amount of 10%, and the cultivation conditions are the same as the first-grade seeds;

[0097]...

Embodiment 3

[0109] A biological compound fertilizer, the number of parts by weight is: 10 parts of Bacillus colloidus, 6 parts of phosphate solubilizing bacteria, 7 parts of potassium solubilizing bacteria, 27 parts of Bacillus subtilis culture, 9 parts of Aspergillus awamori culture, plant Lactobacillus agent 7 servings.

[0110] The preparation of the Bacillus subtilis culture: transfer and cultivate the Bacillus subtilis from the inclined plane, transfer the seed liquid after step-by-step expansion into the fermentation tank, and after the fermentation is completed, the fermentation liquid is filtered and dried to obtain the Bacillus subtilis culture .

[0111] The preparation method of Lactobacillus plantarum agent: transfer and cultivate Lactobacillus plantarum from an inclined plane, transfer the seed liquid after step-by-step expansion into a fermenter, and after the fermentation is completed, the fermented liquid is concentrated to 45% of the original volume by low-temperature neg...

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Abstract

The invention discloses a preparation method of a biological compound fertilizer, belonging to the technical field of agricultural fertilizers. The preparation method of the biological compound fertilizer comprises the following steps: mixing 5-18 parts by weight of Bacillus mucilaginosus, 2-6 parts by weight of phosphate-solubilizing bacterium, 5-10 parts by weight of potassium bacterium, 18-30 parts by weight of Bacillus subtilis nutrient material, 5-10 parts by weight of Aspergillus awamori nutrient material and 3-8 parts by weight of lactobacillus plantarum microbial inoculum, or mixing and granulating. The fertilizer can enhance the drought resistance of the crops; the Bacillus mucilaginosus in the compound strain is prepared by industrially fermenting high-potassium-solubilizing-capacity silicate bacterium screened from crop roots in a specific culture medium, and can secrete auxin substances, gibberellin substances and other growth promoting substances to reinforce the roots and seedlings; the silicon in the soil is decomposed and supplied to the plant, so that the waxy layer of the plant is thickened, thereby enhancing the water retention capacity of the plant; and the fertilizer can promote the formation of the soil aggregate from, keep soil from packing together, destroy the soil capillarity and prevent the soil moisture from evaporation.

Description

technical field [0001] The invention relates to a biological fertilizer processing method, which belongs to the technical field of agricultural fertilizers, in particular to a preparation method of biological compound fertilizer. Background technique [0002] Fertilizer pollution has become a major public hazard in the world today. Agricultural experts have unanimously called for reducing the amount of chemical fertilizers to control the environment. An industry expert and academician Chen Wenxin of China Agricultural University believes that the excessive application of chemical fertilizers in my country has reached its limit. Taking nitrogen fertilizer as an example, my country's average application rate is nearly three times that of the United States and more than eight times that of Australia. This has caused a series of serious problems such as the destruction of soil structure, the reduction of natural biodiversity and ecosystem stability. Dr. Li Jun, director of the...

Claims

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Application Information

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IPC IPC(8): C05F11/08
CPCY02P20/141
Inventor 邵素英孔日祥
Owner 山东省神农生态科技股份有限公司
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