Green microecological compound fertilizer
A technology of micro-ecology and compound fertilizer, which is applied in the direction of organic fertilizers, can solve the problems of unstable product quality and application effect, the overall level of microbial fertilizer industry is not high, and lack of investment in research, so as to prevent soil moisture from evaporating and improve micro The effect of improving the ecological environment and improving soil fertility
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Examples
preparation example Construction
[0041] (1) Preparation of bacterial suspension
[0042] Inoculate the Li-2013 single colony grown after streaking on the plate into the seed medium, culture at 40°C for 12 hours at 100 r / min, take 1 mL of the culture medium, centrifuge, wash twice with normal saline, and resuspend with 9 mL of physiological in salt water.
[0043] (2) Ultraviolet-Lithium Chloride-Diethyl Sulfate Compound Mutagenesis
[0044] The bacterial suspension was placed on a sterile plate, stirred and irradiated for 100s under a UV lamp with a distance of 30cm and a power of 15w. The irradiated bacteria solution was diluted and spread on the lithium chloride plate after gradient dilution, and the bacteria solution without ultraviolet irradiation was diluted and spread on the plate as a control. Wrap the uniformly coated plate above with black cloth or newspaper, incubate at 40°C for 48 hours, screen out the plate with the largest ratio of hydrolysis circle to colony diameter on the plate that grows co...
Embodiment 1
[0063] A kind of green micro-ecological compound fertilizer is provided: the number of parts by weight is as follows: 6 parts of Aspergillus niger culture, 5 parts of colloid bacillus bacteria agent, 5 parts of phosphorus-solubilizing bacillus megaterium bacteria agent, 10 parts of bacillus subtilis culture, awamori Aspergillus culture 6, Lactobacillus plantarum agent 3 parts.
[0064] The strains used are as follows:
[0065] Bacillus subtilis subsp CGMCC7926
[0066] Lactobacillus plantarum CICC20764
[0067] Aspergillus awamori (Aspergillus awamori) CGMCC3.6484
[0068] The preservation number of Aspergillus niger Li-2013-03 is CGMCC NO.7927.
[0069] The preparation method of Awamori Aspergillus agent:
[0070] The technical solution is as follows:
[0071] Activation culture of slant strains: transfer Aspergillus awamori slant strains to the slant medium, and culture at 27°C for 3 days.
[0072] Solid first-level seed culture: pick the Aspergillus awamori slant stra...
Embodiment 2
[0093] Basically the same as embodiment 1
[0094] A kind of green micro-ecological compound fertilizer is provided: the number of parts by weight is: 5 parts of Aspergillus niger culture, 7 parts of colloid bacillus bacteria agent, 6 parts of phosphorus-solubilizing bacillus megaterium bacteria agent, 12 parts of bacillus subtilis culture, awamori Aspergillus culture 6, plantarum lactobacillus agent 2 parts.
PUM
Property | Measurement | Unit |
---|---|---|
Enzyme activity | aaaaa | aaaaa |
Vitality | aaaaa | aaaaa |
Aperture | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com