Preparation method of biofertilizer

A technology of biological bacterial fertilizer and Lactobacillus plantarum, applied in the direction of nitrogen fertilizer, organic fertilizer, fertilization device, etc., can solve the problems of unstable product quality and application effect, insufficient technological innovation, and low overall level of microbial fertilizer industry

Inactive Publication Date: 2015-02-11
BEIJING KEHUITONG WISDOM SCI & TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] my country's microbial fertilizer industry still has the problems of low overall level, insufficient technological innovation, and unstable product quality and application effects.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065] Bacillus subtilis (Bacillus subtilis) Li-2013-02, the strain has been preserved in the General Microbiology Center of China Committee for Culture Collection of Microorganisms (CGMCC for short) on July 15, 2013, the address is: Beichen West Road, Chaoyang District, Beijing, China No. 1 Courtyard No. 3), the preservation number is CGMCC No.7926.

[0066] The characteristic of the strain is that it has high enzyme activity for producing high temperature-resistant α-amylase, and strong heat resistance and acid resistance.

[0067] The high temperature-resistant α-amylase enzyme activity prepared by the strain is 30000-35000u / ml; the applicable temperature range is 105-115°C, the optimum reaction temperature is 110°C, and the enzyme activity is completely stable at 110°C; the applicable reaction pH range is 3.0-7.0, when the pH value is 3.0, the enzyme activity is completely stable, and the optimum reaction pH value is 4.2.

[0068] Described bacterial strain characteristic...

Embodiment 2

[0129] A biological bacterial fertilizer, the number of parts by weight is: 25 parts of Lactobacillus plantarum agent, 1 part of phosphorus-dissolving bacterial agent, 3 parts of potassium-dissolving bacterial agent, 12 parts of Bacillus subtilis culture, 10 parts of Aspergillus awamori culture, polyglutamine acid 6 parts.

[0130] The preparation method of the biological bacterial fertilizer is as follows: mix the Lactobacillus planta agent, the phosphate-dissolving bacteria agent, the potassium-dissolving bacteria agent, the culture of Bacillus subtilis, and the culture of Aspergillus awamori according to the proportion; Control it at 1 mm, then make the fertilizer particles adhere and add polyglutamic acid in a wet state, and then control the temperature at 35 degrees for 1 hour.

[0131] The polyglutamic acid is γ-polyglutamic acid.

[0132] Preparation method of Bacillus subtilis culture:

[0133] Obtaining of fermented liquid: Obtaining Bacillus subtilis fermented liqu...

Embodiment 3

[0147] Embodiment 3 is basically the same as example 1 and 2

[0148] A biological bacterial fertilizer, the number of parts by weight is: 25 parts of Lactobacillus plantarum agent, 2 parts of phosphorus-dissolving bacterial agent, 2 parts of potassium-dissolving bacterial agent, 12 parts of Bacillus subtilis culture, 9 parts of Aspergillus awamori culture, polyglutamic acid Acid 7.

[0149] The preparation method of the biological bacterial fertilizer is as follows: mix the Lactobacillus planta agent, the phosphate-dissolving bacteria agent, the potassium-dissolving bacteria agent, the culture of Bacillus subtilis, and the culture of Aspergillus awamori according to the proportion; Control it at 0.5-2 mm, then make the fertilizer particles adhere to add polyglutamic acid in a wet state, and then control the temperature at 35 degrees for 2 hours.

[0150] The polyglutamic acid is γ-polyglutamic acid.

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Abstract

The invention discloses a preparation method of a biofertilizer, belonging to the technical field of agricultural fertilizers. The preparation method of the biofertilizer comprises the following steps: uniformly mixing plant lactobacillus, phosphate solubilizing bacteria, potassium solubilizing bacteria, a bacillus subtilis culture and an aspergillus awamori culture in proportion; putting the mixture into a granulator to be granulated, controlling the grain sizes between 0.5mm and 2mm, then enabling the fertilizer granules to adhere to added humid polyglutamic acid, then controlling the temperature at 30-40 DEG C and maintaining the temperature for 1-3 hours, wherein the parts by weight of plant lactobacillus, the phosphate solubilizing bacteria, the potassium solubilizing bacteria, the bacillus subtilis culture, the aspergillus awamori culture and polyglutamic acid are respectively 20-35 parts, 1-2 parts, 2-4 parts, 10-15 parts, 8-15 parts and 5-8 parts. The fertilizer can be used for enhancing the drought resistance capabilities of crops, improving the physical properties of soil, improving the use efficiency, regulating acid-base balance of soil and reducing pollution of harmful substances in soil, such as nitrite and is conductive to increasing soil fertility.

Description

technical field [0001] The invention belongs to the technical field of agricultural fertilizers, in particular to a method for preparing biological bacterial fertilizers. Background technique [0002] Microbial fertilizer is a microbial living product that uses microbial life activities and its products to cause crops to obtain specific fertilizer effects. It can improve soil fertility, improve fertilizer utilization, inhibit crops from absorbing harmful substances such as heavy metals and pesticides, and purify and restore soil. It plays an irreplaceable role in promoting the utilization of crop stalks and urban waste, and improving the quality of agricultural products. Microbial fertilizers have a variety of fertility and efficacy of chemical fertilizers, organic fertilizers, and beneficial biological bacteria. They are ideal fertilizers for activating fertilizer nutrients, improving fertilizer effects, improving crop quality, and promoting agricultural production and effi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05F11/08
CPCC05C11/00C05G3/80C05F11/08C05G5/12
Inventor 孔日祥李建树
Owner BEIJING KEHUITONG WISDOM SCI & TECH CO LTD
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