Compound fertilizer and preparation method thereof
A compound fertilizer and culture technology, applied in the field of compound fertilizer, can solve the problems of unstable product quality and application effect, low overall level of microbial fertilizer industry, and insufficient technological innovation, so as to enhance the ability to resist diseases and insect pests and improve the micro-ecological environment. , the effect of enhancing drought resistance
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Examples
preparation example Construction
[0030] (1) Preparation of bacterial suspension
[0031] Inoculate the Li-2013 single colony grown after streaking on the plate into the seed medium, culture at 40°C for 12 hours at 100 r / min, take 1 mL of the culture medium, centrifuge, wash twice with normal saline, and resuspend with 9 mL of physiological in salt water.
[0032] (2) Ultraviolet-Lithium Chloride-Diethyl Sulfate Compound Mutagenesis
[0033] The bacterial suspension was placed on a sterile plate, stirred and irradiated for 100s under a UV lamp with a distance of 30cm and a power of 15w. The irradiated bacteria solution was diluted and spread on the lithium chloride plate after gradient dilution, and the bacteria solution without ultraviolet irradiation was diluted and spread on the plate as a control. Wrap the uniformly coated plate above with black cloth or newspaper, incubate at 40°C for 48 hours, screen out the plate with the largest ratio of hydrolysis circle to colony diameter on the plate that grows co...
Embodiment 1
[0052] A kind of compound fertilizer, the array of parts by weight becomes: 8 parts of yeast inoculum, 8 parts of colloid bacillus inoculum, 8 parts of Bacillus megaterium phosphate solubilizing inoculum, 12 parts of Bacillus subtilis culture, 3 parts of Aspergillus awamori culture, Plant Lactobacillus agent 3 parts.
[0053] The strains used are as follows:
[0054] Bacillus subtilis subsp CGMCC7926
[0055] Lactobacillus plantarum CICC20764
[0056] Aspergillus awamori (Aspergillus awamori) CGMCC3.6484
[0057] Saccharomyces cerevisiae selected cicc1526, cicc1413, and the strains were purchased from China Industrial Microorganism Culture Collection Center.
[0058] For the production method of yeast inoculum, refer to Xiao Dongguang's "Production and Application Technology of Saccharomyces cerevisiae Active Dry Yeast", published by Inner Mongolia People's Publishing House in 1994.
[0059] The preparation method of Awamori Aspergillus agent:
[0060] The technical solut...
Embodiment 2
[0083] Basically the same as embodiment 1
[0084] A kind of compound fertilizer, the array of parts by weight is: 9 parts of saccharomyces agent, 7 parts of colloid bacillus bacteria agent, 7 parts of Bacillus megaterium phosphate solubilizing agent, 13 parts of Bacillus subtilis culture, 2 parts of Aspergillus awamori culture, Plant Lactobacillus agent 2 parts.
PUM
Property | Measurement | Unit |
---|---|---|
pore size | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com