Biological compound fertilizer
A bio-compound fertilizer and culture technology, applied in the field of bio-fertilizer and bio-compound fertilizer, can solve the problems of single microbial strain, limited use method, lack of fermentation metabolites, etc., to improve soil physical properties, prevent soil moisture from evaporating, The effect of improving soil aggregate structure
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Embodiment 1
[0028] Bacillus subtilis (Bacillus subtilis) Li-2013-02, the strain has been preserved in the General Microorganism Center of China Committee for Culture Collection of Microorganisms (CGMCC for short) on July 15, 2013, the address is: Beichen West Road, Chaoyang District, Beijing, China No. 1 Courtyard No. 3), the preservation number is CGMCC No.7926.
[0029] The characteristic of the strain is that it has high enzyme activity for producing high temperature-resistant α-amylase, and strong heat resistance and acid resistance.
[0030] The high temperature-resistant α-amylase enzyme activity prepared by the strain is 30000-35000u / ml; the applicable temperature range is 105-115°C, the optimum reaction temperature is 110°C, and the enzyme activity is completely stable at 110°C; the applicable reaction pH range is 3.0-7.0, when the pH value is 3.0, the enzyme activity is completely stable, and the optimum reaction pH value is 4.2.
[0031] Described bacterial strain characteristi...
Embodiment 2
[0092] A biological compound fertilizer, the number of parts by weight is: 12 parts of colloidal bacillus, 5 parts of phosphorus solubilizing bacteria, 8 parts of potassium solubilizing bacteria, 25 parts of bacillus subtilis culture, 7 parts of aspergillus awamori culture, plant lactobacillus agent 6 servings.
[0093] Preparation method of Bacillus subtilis culture:
[0094] Obtaining of fermented liquid: Obtaining Bacillus subtilis fermented liquid by step-by-step expansion culture of slanted strains;
[0095] (1) Primary seed cultivation: Inoculate the slant strain of Bacillus subtilis into a 500-mL shaker flask with a medium capacity of 100 mL, a rotary shaker at 180 rpm, a cultivation temperature of 30°C, and a cultivation time of 24 hours;
[0096] (2) Secondary seed cultivation: Put the first-grade seeds into 500 ml second-grade seed shake flasks according to the inoculation amount of 10%, and the cultivation conditions are the same as the first-grade seeds;
[0097]...
Embodiment 3
[0109] A biological compound fertilizer, the number of parts by weight is: 10 parts of Bacillus colloidus, 6 parts of phosphate solubilizing bacteria, 7 parts of potassium solubilizing bacteria, 27 parts of Bacillus subtilis culture, 9 parts of Aspergillus awamori culture, plant Lactobacillus agent 7 servings.
[0110] The preparation of the Bacillus subtilis culture: transfer and cultivate the Bacillus subtilis from the inclined plane, transfer the seed liquid after step-by-step expansion into the fermentation tank, and after the fermentation is completed, the fermentation liquid is filtered and dried to obtain the Bacillus subtilis culture .
[0111] The preparation method of Lactobacillus plantarum agent: transfer and cultivate Lactobacillus plantarum from an inclined plane, transfer the seed liquid after step-by-step expansion into a fermenter, and after the fermentation is completed, the fermented liquid is concentrated to 45% of the original volume by low-temperature neg...
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