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Aspergillus awamori, and microbial inoculant and application thereof

A technology of microbial agent, Aspergillus awamori, which is applied in the field of agricultural microorganisms, can solve the problems of small species and quantity, and achieve the effect of remarkable control effect and less resistance to pesticides

Active Publication Date: 2014-09-03
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the types and quantities of antagonistic microorganisms currently available are still relatively small, mainly including Trichoderma fungi, non-pathogenic Fusarium oxysporum, Paecilomyces lilacinus, Bacillus, etc. It is of great significance for the control of cucumber wilt

Method used

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  • Aspergillus awamori, and microbial inoculant and application thereof
  • Aspergillus awamori, and microbial inoculant and application thereof
  • Aspergillus awamori, and microbial inoculant and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] The isolation and screening of embodiment 1 bacterial classification:

[0031] (1-1) Preparation of PDA medium 、

[0032] The PDA medium: potato 200g, glucose 20g, agar 15-20g, H 2 O 1000ml, pH natural. Wash and peel the potatoes first, then weigh 200g of potatoes and cut them into small pieces, add water to boil (boil for 20-30 minutes), filter with eight layers of gauze, heat, then add agar according to the actual experiment needs, continue to heat and mix well , after the agar is dissolved, add glucose, stir evenly, and then make up water to 1000ml after cooling down a bit, divide into test tubes or Erlenmeyer flasks, stopper, bandage, sterilize at 121°C for about 20 minutes, take out the test tubes and put them on an inclined plane or put them on a flat plate , and store for later use after cooling and solidification.

[0033] The PDA plates and PDA slopes used in the experiment were prepared by conventional operation methods.

[0034] (1-2) Preparation of soi...

Embodiment 2

[0047] Example 2 Screening of cucumber fusarium wilt antagonistic fungi:

[0048] (2-1) Solid medium: prepare PDA medium: same as (1-1) in Example 1.

[0049] (2-2) Activation of Cucumber Fusarium Wilt Pathogenic Bacteria

[0050]Fusarium oxysporum, the pathogenic bacterium of cucumber wilt preserved at low temperature, was inserted into PDA slant medium for activation culture, cultured in an incubator at 28°C for 4 days, and set aside.

[0051] (2-3) Plate antagonism test

[0052] Inoculate the cultured and activated cucumber wilt pathogenic bacteria into the center of the PDA plate, and inoculate the isolated fungi around the plate at the same time, inoculate 4 isolated fungi on each plate, and place them in a fungal incubator at 28°C for cultivation after inoculation. After 48 hours, observe the experimental results every 24 hours, mainly to investigate the presence and size of the inhibition zone. According to the size of the inhibition zone, efficient antagonistic mi...

Embodiment 3

[0053] The preparation of embodiment 3 microbial bacterial agents:

[0054] (3-1) Activation culture of strain 2-17: Inoculate the slant preservation of the isolated Aspergillus awamori (Aspergillus awamori) 2-17 strain on the PDA slant, place it in an incubator at 28°C for 4 days, and then inoculate PDA as the inoculum After inoculation, place the PDA plate in an incubator at 28°C for 4-7 days until spores are formed on the mycelium.

[0055] (3-2) Preparation of solid medium: use bran as raw material, adjust moisture content to 40%-50%, autoclave at 121°C for 20min,

[0056] The bran after cooling was used as a solid medium for Aspergillus awamori (Aspergillus awamori) 2-17.

[0057] (3-3) Preparation of solid microbial agent: Wash the spores and mycelia of Aspergillus awamori 2-17 on the PDA plate with sterile water and inoculate them on the solid medium as an inoculum, and inoculate them at 28°C Cultivate for 7 days to carry out sufficient fermentation and spore produc...

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Abstract

The invention discloses an Aspergillus awamori 2-17 strain for preventing and treating fusarium wilt of cucumber, which is collected to Common Microorganism Center of China Committee for Culture Collection of Microorganisms positioned in Yard 1 No.3, Beichenxi Road, Chaoyang District, Beijing City on 11th January, 2013, wherein the collection number is CGMCC No.7127. The invention also discloses a microbial inoculant prepared from the strain and a preparation method thereof. The Aspergillus awamori 2-17 and microbial inoculant thereof have obvious preventing and treating effects on fusarium wilt of cucumber, can control the morbidity within 5%, can not easily generate drug resistance, are safe for human and livestock, can not pollute water and soil, are harmless to crops, and provide a new efficient microbial way for preventing and treating fusarium wilt of cucumber.

Description

technical field [0001] The invention specifically relates to Aspergillus awamori for preventing and treating cucumber wilt, a microbial agent containing the aspergillus awamori, and its application in preventing and treating cucumber wilt, belonging to the technical field of agricultural microorganisms. Background technique [0002] Cucumber wilt is a worldwide soil-borne fungal disease caused by the infection of Fusarium oxysporum f.sp. and duct damage, eventually causing the cucumber to shrivel, wilt and even die. In many vegetable bases, the annual incidence of cucumber wilt is 10%-30%, and it can reach 80%-90% in severe disease years. Generally, the yield is reduced by 30%-50%. In severe cases, the harvest is completely destroyed, which seriously affects the yield and quality of cucumbers. [0003] At present, chemical methods are mainly used in the prevention and control of cucumber wilt in countries around the world. Although chemical methods have suppressed the occur...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01N63/04A01P3/00C12R1/665
Inventor 姚燕来朱凤香薛智勇陈晓旸洪春来王卫平
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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