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Method of preparing yellow ginger diosgenin by microbial conversion method

A technology for the transformation of turmeric diosgenin and microorganisms, which is applied in the directions of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve saponin loss, hinder the hydrolysis of microorganisms on saponin, and the fermentation conversion rate cannot reach ideal requirements, etc. problems, to achieve the effect of strengthening pertinence, reducing the effect of wrapping, and improving efficiency

Inactive Publication Date: 2014-12-24
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] In recent years, researchers have found that some microorganisms such as Aspergillus oryzae and Trichoderma can convert the saponins in turmeric into diosgenin, but the fermentation conversion rate cannot meet the ideal requirements, because most of the substrates in the fermentation medium are turmeric Powder or turmeric saponins extracted by absolute ethanol. On the one hand, the content of starch and cellulose in turmeric powder accounts for more than 80%, which hinders the hydrolysis of saponins by microorganisms; on the other hand, absolute ethanol can only extract Some alcohol-soluble saponins, water-soluble saponins will be lost

Method used

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  • Method of preparing yellow ginger diosgenin by microbial conversion method
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  • Method of preparing yellow ginger diosgenin by microbial conversion method

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Embodiment 1

[0035] A method for preparing turmeric diosgenin by microbial conversion method, comprising the following steps:

[0036] (1) The carbon source in seed activation medium and fermentation medium is turmeric saponin, and the optimal extraction process of turmeric saponin was obtained by optimizing the response surface methodology. Take 10g of turmeric powder and add 75% (v / v) ethanol according to the solid-liquid ratio of 1:5 (g / ml), and extract the turmeric powder three times in a 200r / min shaker water bath at 70°C to obtain the carbon source turmeric saponin, Used in seed activation medium and fermentation medium.

[0037] (2) Inoculate Aspergillus awamori for fermentation transformation. Inoculate the Aspergillus awamori obtained by screening on the seed activation medium and cultivate it for 5 days. The composition of the seed activation medium is: carbon source turmeric saponin 2g / L, peptone 6g / L, potassium dihydrogen phosphate 1 / L, MgSO 4 ·7H 2 O0.5g / L, potassium chlori...

Embodiment 2

[0041] A method for preparing turmeric diosgenin by microbial conversion method, comprising the following steps:

[0042] (1) The carbon source in seed activation medium and fermentation medium is turmeric saponin, and the optimal extraction process of turmeric saponin was obtained by optimizing the response surface methodology. Weigh 10g of turmeric powder and add 80% (v / v) ethanol according to the solid-liquid ratio of 1:6 (g / ml), and extract the turmeric powder three times in a 200r / min shaker water bath at 75°C to obtain the carbon source turmeric saponin, Used in seed activation medium and fermentation medium.

[0043](2) Inoculate Aspergillus awamori for fermentation transformation. The Aspergillus awamori obtained by screening was inoculated in the seed activation medium and cultivated for 7 days. The composition of the seed activation medium was: turmeric saponin 3g / L, sodium nitrate 3g / L, potassium dihydrogen phosphate 1.5g / L, magnesium sulfate heptahydrate 1g / L , P...

Embodiment 3

[0047] A method for preparing turmeric diosgenin by microbial conversion method, comprising the following steps:

[0048] (1) The carbon source in seed activation medium and fermentation medium is turmeric saponin, and the optimal extraction process of turmeric saponin was obtained by optimizing the response surface methodology. Weigh 10g of turmeric powder and add 70% (v / v) ethanol according to the solid-liquid ratio of 1:4 (g / ml), and extract the turmeric powder three times in a 200r / min shaker water bath at 75°C to obtain the carbon source turmeric saponin, Used in seed activation medium and fermentation medium.

[0049] (2) Inoculate Aspergillus awamori for fermentation transformation. The Aspergillus awamori obtained by screening was inoculated in the seed activation medium and cultivated for 5 days. The composition of the seed activation medium was: turmeric saponin 4g / L, peptone 6g / L, potassium dihydrogen phosphate 2g / L, magnesium sulfate heptahydrate 2g / L, Potassium ...

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Abstract

The invention provides a method of preparing yellow ginger diosgenin by a microbial conversion method. The method specifically comprises the following steps: extracting yellow ginger saponin by 75% alcohol; adding the yellow ginger saponin as well as a nitrogen source and other inorganic salt ions to prepare a seed activation culture medium and a fermentation culture medium; inoculating aspergillus awamori and fermenting a shaking bed; and adding methanol to ultrasonically oscillate and extract diosgenin produced by fermentation. The method disclosed by the invention is high conversation ratio of yellow ginger diosgenin, environmentally friendly and capable of recycling the methanol; and moreover, the defects that the existing acid hydrolysis process wastes raw materials, is severe in pollution and high in investment cost are solved.

Description

technical field [0001] The invention relates to a method for preparing turmeric diosgenin, in particular to a method for preparing turmeric diosgenin by a microbial transformation method. Background technique [0002] Diosgenin is an important basic raw material for the production of steroid drugs. Diosgenin produced by the metabolism of intestinal flora after oral administration of diosgenin is the real active ingredient that plays a role and is an important pharmaceutical and chemical raw material for the synthesis of steroid hormone drugs and steroidal contraceptives. It also has anti-aging, anti-tumor, It can kill snails and prevent cardiovascular diseases. [0003] At present, the main processes for producing diosgenin at home and abroad include direct acid hydrolysis and pre-fermentation acid hydrolysis. These two processes both have the process of acid hydrolysis, that is, the acid hydrolysis method has the advantages of short hydrolysis time and low cost, but the d...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P33/20C12R1/665
Inventor 吴晖陈宇任尧胡博涵
Owner SOUTH CHINA UNIV OF TECH
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