Preparation method for great burdock fruit extract through microbial combined fermentation
A combined fermentation and microorganism technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of changing structure, complicated process, isomerization, etc., and achieve the goal of changing drug properties, simple process and low cost Effect
Active Publication Date: 2013-08-07
武汉市畜牧兽医科学研究所
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[0003] At present, there have been reports in China on the in vitro enzymatic hydrolysis of extracted arctiin by using helicase, but there are some shortcomings: the process is complicated and the cost is too hi
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The invention specifically discloses a preparation method for a great burdock fruit extract through combined microbial fermentation, which belongs to the technical field of preparation of traditional Chinese medicinal material extracts through microbial fermentation. According to the invention, the great burdock fruit extract is prepared through combined fermentation of Aspergillus awamori var. CICC2203 and Trichoderma reesei CICC40932, optimization study is carried out on fermentation process conditions, so a conversion rate of arctiin in great burdock fruit into arctigenin is substantially improved and reaches 99.9%, the problem of a low conversion rate of arctiin into arctigenin is effectively overcome, and a technical problem of industrial production of the great burdock fruit extract is overcome.
Description
technical field [0001] The invention relates to the technical field of methods for preparing Chinese herbal extracts by microbial fermentation, in particular to a method for preparing burdock fruit extracts by combined fermentation with microorganisms. Background technique [0002] Burdock fruit (Fructus Arctii) mainly contains lignan compounds such as arctiin and arctigenin (ACT). Arctiin is decomposed into arctigenin in the body and produces many pharmacological effects. Arctigenin has significant antibacterial, antiviral, antitumor, anti-inflammatory and calcium antagonistic activities. And arctigenin is rich in sources, and its precursor arctiin is relatively high in the traditional Chinese medicine burdock and burdock root fruit, which has high value for new drug development. [0003] At present, there have been reports in China on the in vitro enzymatic hydrolysis of extracted arctiin by using helicase, but there are some shortcomings: the process is complicated and ...
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Inventor 陈洁何斌陆征操继跃钱运国杨文海童新红叶胜强韩艳云童伟文陶弼菲夏瑜孙书林
Owner 武汉市畜牧兽医科学研究所
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