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Efficient micro-ecological bio-fertilizer and preparation method thereof

A micro-ecological and biological fertilizer technology, applied in organic fertilizers, fertilization devices, fertilizer mixtures, etc., can solve the problems of low overall level of the microbial fertilizer industry, unstable product quality and application effect, and insufficient technological innovation.

Inactive Publication Date: 2015-04-01
邵素英
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Due to the long-term lack of investment in microbial fertilizer research in China, my country's microbial fertilizer industry still has problems such as low overall level, insufficient technological innovation, and unstable product quality and application effects.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0084] (1) Preparation of bacterial suspension

[0085] Inoculate the Li-2013 single colony grown after streaking on the plate into the seed medium, culture at 40°C for 12 hours at 100 r / min, take 1 mL of the culture medium, centrifuge, wash twice with normal saline, and resuspend with 9 mL of physiological in salt water.

[0086] (2) Ultraviolet-Lithium Chloride-Diethyl Sulfate Compound Mutagenesis

[0087] The bacterial suspension was placed on a sterile plate, stirred and irradiated for 100s under a UV lamp with a distance of 30cm and a power of 15w. The irradiated bacteria solution was diluted and spread on the lithium chloride plate after gradient dilution, and the bacteria solution without ultraviolet irradiation was diluted and spread on the plate as a control. Wrap the uniformly coated plate above with black cloth or newspaper, incubate at 40°C for 48 hours, screen out the plate with the largest ratio of hydrolysis circle to colony diameter on the plate that grows co...

Embodiment 1

[0106] The high-efficiency micro-ecological biological fertilizer is composed of 20 parts by weight of Lactobacillus plantarum agent, 13 parts of Aspergillus niger culture, 3 parts of Bacillus colloidus bacteria agent, 2 parts of Bacillus megaterium phosphate solubilizing agent, and Bacillus subtilis bacteria powder 10, Aspergillus awamori culture 3, polyglutamic acid 2.

[0107] The polyglutamic acid is γ-polyglutamic acid.

[0108] The plantarum lactobacillus is CGMCC9405.

[0109] The preparation method of described high-efficiency micro-ecological biological fertilizer is as follows:

[0110] Mix Lactobacillus plantarum agent, Aspergillus niger culture, Bacillus coliformis agent, Bacillus megaterium phosphate solubilizing agent, Bacillus subtilis agent and Aspergillus awamori culture according to the proportion, and use fertilizer granulator to granulate , the particle size is between 0.5-2mm, and then the above-mentioned fertilizer granules are mixed with polyglutamic a...

Embodiment 2

[0140] Basically the same as embodiment 1

[0141] The high-efficiency micro-ecological biological fertilizer is composed of 13 parts by weight of Lactobacillus plantarum agent, 16 parts of Aspergillus niger culture, 1 part of Bacillus colloidus agent, 3 parts of Bacillus megaterium phosphate solubilizer, and Bacillus subtilis powder 15, Aspergillus awamori culture 2, polyglutamic acid 1.

[0142] The polyglutamic acid is γ-polyglutamic acid.

[0143] The plantarum lactobacillus is CGMCC9405.

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Abstract

The invention discloses an efficient micro-ecological bio-fertilizer and its preparation method. The invention relates to a micro-ecological fertilizer and belongs to the technical field of agricultural fertilizers. The efficient micro-ecological bio-fertilizer comprises the following ingredients, by weight, 13-26 parts of lactobacillus plantarum, 10-16 parts of aspergillus niger culture, 1-5 parts of bacillus mucilaginosus microbial inoculum, 1-3 parts of phosphate-solubilizing bacillus megaterium microbial inoculum, 8-15 parts of bacillus subtilis powder, 2-6 parts of aspergillus awamori culture and 1-3 parts of polyglutamic acid. polyglutamic acid on the surface of the fertilizer particles can absorb moisture during the actual use process so as to form hydrogel. Thus, a moisture environment is provided for rapid growth and propagation of the granule interior fertilizer. Meanwhile, the effect of slowly releasing fertility of the bio-fertilizer also can be achieved, and irrigation water capacity in the growth stage of crops can effectively be reduced by more than 13%.

Description

technical field [0001] The invention relates to a micro-ecological fertilizer and belongs to the technical field of agricultural fertilizers. Background technique [0002] The efficacy of microbial fertilizers is mainly achieved through the synergistic effect on traditional chemical fertilizers and organic fertilizers, the improvement and activation of soil, and the physiological effects of microorganisms. Therefore, microbial fertilizers have a variety of fertility and efficacy of chemical fertilizers, organic fertilizers, and beneficial biological bacteria. They are ideal fertilizers for activating fertilizer nutrients, improving fertilizer effects, improving crop quality, and promoting agricultural production and efficiency. Microbial fertilizer is a living fertilizer, and its function is mainly completed by the life activities of a large number of beneficial microorganisms it contains. Only when the life activities of these beneficial microorganisms are completed. Only...

Claims

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Application Information

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IPC IPC(8): C05G3/00
CPCC05F11/08
Inventor 邵素英李建树
Owner 邵素英
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