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Biological complex enzyme production method

A biological compound enzyme and production method technology, applied in the field of biological compound enzyme products, can solve the problems that the role of organic fertilizer is difficult to fully exert, and the soil structure is not suitable for improvement, and achieve the effect of simple use method

Active Publication Date: 2011-12-14
JINAN ZEYUAN BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is difficult to give full play to the role of organic fertilizer, and the soil structure should not be improved

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] The composition of the product of the present invention consists of: Bacillus subtilis 8.0×10 per gram of product 8 A, Aspergillus awamori 4.0×10 7 1, protease 200IU, β-glucanase 220IU, xylanase 100IU, cellulase 110IU, amylase 270IU.

[0027] The concrete production method step of product of the present invention is as follows:

[0028] Bacteria culture preparation:

[0029] Bacillus subtilis agent culture preparation: Bacillus subtilis agent preparation: transfer and cultivate Bacillus subtilis from the slope, transfer the seed liquid after step-by-step expansion into the fermenter, and centrifuge after fermentation to obtain wet bacteria and fermentation liquid; After the fermentation is completed, the fermented liquid is vacuum-concentrated to 50% of the original volume by low-temperature negative pressure to obtain a bacterial concentrated liquid. Add carrier: Add the mixed carrier to the concentrated solution and mix evenly; the weight ratio of the concentrated ...

Embodiment 2

[0060] Embodiment 2: basically with embodiment 1.

[0061] The product of the present invention consists of: Bacillus subtilis 4.0×10 per gram of product 8 A, Aspergillus awamori 4.0×10 7 1, protease 150IU, β-glucanase 250IU, xylanase 50IU, cellulase 100IU, amylase 250IU.

[0062] The concrete production method step of product of the present invention is as follows:

[0063] Bacteria culture preparation:

[0064] Bacillus subtilis agent culture preparation: Bacillus subtilis agent preparation: transfer and cultivate Bacillus subtilis from the slope, transfer the seed liquid after step-by-step expansion into the fermenter, and centrifuge after fermentation to obtain wet bacteria and fermentation liquid; After the fermentation is completed, the fermented liquid is vacuum-concentrated to 50% of the original volume by low-temperature negative pressure to obtain a bacterial concentrated liquid. Add carrier: Add the mixed carrier to the concentrated solution and mix evenly; the ...

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Abstract

The invention discloses a production method of a biological compound enzyme, belongs to the technical field of biology, and particularly relates to a biological compound enzyme product. The production method of the product comprises the following specific steps: respectively culturing and preparing aspergillus awamori culture, lactobacillus plantarum agent and bacillus subtilis inoculant, whereinthe inoculant is compounded and mixed in the following proportion: 50 to 80 parts of the aspergillus awamori culture, 5 to 10 parts of the lactobacillus plantarum agent, and 20 to 40 parts of the bacillus subtilis inoculant. Years of experiments prove that: the soil biological enzyme can promote soil loosening of an active soil layer, and improve soil fertility so as to build an excellent soil environment suitable for crop root growth; transformation of the soil biological enzyme plays remarkable effects in reducing agricultural production cost, reducing fertilizer use, recovering soil ecological land fertility, effectively increasing crop yield and the like; therefore, the efficacy of organic matters in soil can be sufficiently exerted.

Description

Technical field: [0001] The invention belongs to the field of biotechnology, in particular to biological complex enzyme products. Background technique [0002] Biological complex enzymes are widely used in textile, food processing, medicine, fermentation, tobacco aging, feed and other industries. However, it is the first time that it is applied to improve soil and promote the growth of crops, and there is no data report. [0003] The reasonable compatibility of unconventional extracellular complex enzymes produced by industrial microorganisms and intracellular complex enzymes can effectively promote the growth of other microorganisms. And it has a positive effect on the production of most organisms and metabolites, and at the same time has a significant activation effect on microbial enzymes, and puts forward the idea of ​​developing preparation products for the fermentation industry. [0004] At the same time, a compound enzyme preparation for soil microorganisms (soil bi...

Claims

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Application Information

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IPC IPC(8): C12N11/14C12N11/10C12N1/20C12N1/14C09K17/00C12R1/665C12R1/125C12R1/25C09K101/00
Inventor 李绩李政孔日祥
Owner JINAN ZEYUAN BIOTECH
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