Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Aspergillus clavatus for producing feruloyl esterase and application thereof

A technology of ferulic acid esterase and Aspergillus clavus, which is applied in the biological field, can solve problems such as unfavorable sustainable development, and achieve the effects of easy cultivation and preservation, high enzyme activity, and rapid reproduction

Active Publication Date: 2014-10-08
华工利亚(佛山)生物科技有限责任公司
View PDF3 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Although the chemical method is simple to operate and has high efficiency, the subsequent treatment of the extraction waste liquid is not conducive to sustainable development

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Aspergillus clavatus for producing feruloyl esterase and application thereof
  • Aspergillus clavatus for producing feruloyl esterase and application thereof
  • Aspergillus clavatus for producing feruloyl esterase and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Primary screening of Aspergillus clavus, high-yielding strains were screened by Oxford cup transparent circle screening experiment.

[0041] Collect soil samples from the South China Botanical Garden, weigh 10g of the sample, put it into a 300mL Erlenmeyer flask filled with 90mL of sterile saline containing glass beads, shake for 20 minutes, and make a 10-fold incremental serial dilution of the sample homogeneous solution 1:10, 1:100 , 1:1000, and 1:10000, take 1.0mL each of the diluted sample homogeneous solution 1:100, 1:1000, and 1:10000 and add it to a sterile plate; add 15mL of PDA medium and mix evenly, let it stand for 1min, and put it in a sterile plate. Inverted culture at 28°C for 72 hours, transfer the single colony on the medium to the slant PDA medium and culture for 72 hours at 28°C, then transfer to the primary screening medium containing ethyl ferulic acid and culture for 60 hours at 28°C to screen for ferulic acid production Ester strains, spare;

[00...

Embodiment 2

[0044] Microbiological characteristics and identification of strains

[0045] The colony of Aspergillus clavus grows slowly on Cha's culture medium, and the diameter is 30mm after 7 days of culture; the texture is velvety to powdery, the mycelium is white, the conidia are dark blue-green in structure, and there is no exudate. The growth rate of colony on malt juice agar is relatively fast, and the diameter is 45mm after 7 days of cultivation. The colony was cultured on Saccharomyces chagheri paste agar for 7 days, with a diameter of 40 mm, velvety texture, and a slightly thick bottle; conidia had many structures, grayish blue, thinner, white edges, and no exudate.

[0046] Under the microscope, it can be observed that the conidia heads are rod-shaped when they are young, and the conidiophores occur in the matrix, and the lengths of the spores vary; the apical capsule gradually expands from the top of the spore to a club shape, with a diameter of 50-60 mm, and a sporulation str...

Embodiment 3

[0049] Determination of the Release Amount of Bound Ferulic Acid by Solid State Fermentation

[0050] According to the formula of the seed culture medium, weigh 10g of bran and add 10g of water and mix it into a 500mL triangular flask. After the seed culture medium is sterilized, inoculate Aspergillus clavus ( Aspergillus clavatus ) Tobacco slant strain, the inoculum amount was 0.5%, and after culturing at 28°C for 3 days, the seeds of Aspergillus clavus were obtained;

[0051] According to the formula of the fermentation medium, add 200g of bran and 200g of water, mix and sterilize, put it on a porcelain plate to cool, control the thickness of wheat bran within 2.5cm, and the inoculation amount of Aspergillus clavus seeds is 0.5% (the bacterial content is 1~2×10 9 Individual / g), culture temperature is 28 ℃, carry out ventilated culture, fermentation time is 48h. Solid-state fermented bran was leached in a water bath at 40°C for 60 min at the ratio of 15 g to 90 mL of water...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a strain of Aspergillus clavatus for producing feruloyl esterase and application thereof. The Aspergillus clavatus has the category name of Aspergillus clavatus fumigatus, is collected by China center for type culture collection, called CCTCC for short, at December 8, 2013, has the collection number of CCTCCNO: M2013641 and is applied to the production of feruloyl esterase. According to the strain provided by the invention, a method that feruloyl esterase is produced through fermenting by taking wheat bran as a fermentation substrate and bound-type phenolic and ferulic acids are obtained through rupturing ester bonds between the polysaccharide and lignin of plant cell walls and ferulic acid by the feruloyl esterase, is adopted, and the condition that microorganisms are short in growth cycle, are not restricted to climate and are easily subjected to large-scale production is utilized, so that a novel biological method for extracting bound-type phenolic acid substances, such as ferulic acid is provided.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a ferulic esterase-producing Aspergillus clavus and application thereof. Background technique [0002] Wheat is one of the crops with the most extensive planting area in the world, accounting for about 26% of the planting area of ​​grain crops. The annual output of wheat in my country is high, and the annual output of wheat bran can reach more than 20 million tons. However, the development of bran has always been Utilization is seldom, and it is basically only used as feed raw materials. Wheat bran mainly contains non-starch polysaccharides, starch, fat, lignin, protein, vitamins and phenolic substances, among which arabinoxylan-based non-starch polysaccharides contain 46%, which is the main component of wheat cell walls. Element. Bran also contains a certain amount of phenolic acids, such as ferulic acid, p-coumaric acid, caffeic acid, sinapic acid and syringic acid Am...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12N9/18C12R1/66
Inventor 吴晖胡博涵赖富饶刘素纯曾岚
Owner 华工利亚(佛山)生物科技有限责任公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com