A strain of Bacillus Veles that can produce ferulic acid esterase and its application

A technology of Bacillus Velez and ferulic acid esterase, applied in the field of microorganisms, can solve the problems of limiting the industrialization process of ferulic acid and low production of ferulic acid esterase

Active Publication Date: 2021-10-19
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the low yield of ferulic acid esterase produced by the existing strains that can produce ferulic acid esterase, this greatly limits the industrialization process of producing ferulic acid by biological enzyme method

Method used

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  • A strain of Bacillus Veles that can produce ferulic acid esterase and its application
  • A strain of Bacillus Veles that can produce ferulic acid esterase and its application
  • A strain of Bacillus Veles that can produce ferulic acid esterase and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Embodiment 1: the acquisition of Bacillus Velez SK52.002

[0040] Specific steps are as follows:

[0041] 1. Separation and purification

[0042] Take the soil from Wuxi City, Jiangsu Province as a sample, take 1.0g of the sample and put it into 10mL of sterile water filled with glass beads, shake at 30°C and 200r / min for 30min on a shaker to fully separate the bacteria and obtain a mixed bacteria solution ; Draw 0.5mL of mixed bacterial solution and add in a 10mL centrifuge tube that 4.5mL of normal saline is housed under aseptic environment, obtain 10 -1 Diluent, repeat the above dilution steps to obtain 10 -2 、10 -3 、10 -4 、10 -5 、10 -6 Diluent; pipette 100μL dilution gradient of 10 -3 、10 -4 、10 -5 、10 -6 Spread the diluted solution on the strain isolation medium, and incubate it upside down in a constant temperature incubator at 30°C for 24 to 48 hours to obtain a diluted coating plate; pick a single colony on the diluted coating plate and streak it on the ...

Embodiment 2

[0048] Embodiment 2: the production of ferulic acid esterase

[0049] Specific steps are as follows:

[0050] Dip the bacterium solution of Bacillus velezensis SK52.002 obtained in Example 1, streak on the LB solid medium, and culture it upside down in a constant temperature and humidity incubator at 30°C for 24 to 48 hours to obtain a single colony Pick a single colony and inoculate it into the seed medium, and cultivate it in a shaker at 30°C and 200r / min for 48h to obtain the seed liquid; inoculate the seed liquid into the fermentation medium according to the inoculum size of 5% (v / v) , and fermented in a shaker at 30° C. and 200 r / min for 16 hours to obtain a fermentation broth.

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Abstract

The invention discloses a ferulic esterase-producing bacillus velei and application thereof, belonging to the technical field of microbes. The invention provides a strain of Bacillus velezensis (Bacillus velezensis) SK52.002. This Bacillus velezensis SK52.002 can highly produce ferulic acid esterase, which is embodied in the following steps: the Bacillus velezensis (Bacillus velezensis) ) SK52.002 was inoculated into the fermentation medium for fermentation, and after 16 hours of fermentation, the enzyme activity of ferulic acid esterase in the fermentation broth could be as high as 12mU / mL. Therefore, Bacillus velezensis SK52.002 in It has great application prospects in the production of ferulic acid esterase and ferulic acid.

Description

technical field [0001] The invention relates to a ferulic esterase-producing bacillus velei and its application, belonging to the technical field of microbes. Background technique [0002] Ferulic acid (FA) widely exists in plant cell walls, and is connected with polysaccharides, cellulose, and lignin through ester bonds or ether bonds to form a complex network skeleton structure in the cell wall to maintain the integrity of the cell wall. At the same time reduce the biodegradation rate. [0003] Ferulic acid is recognized as a safe antioxidant in the world, and it is included in food additives in Japan, the United States and other countries. In addition, ferulic acid also has the functions of ultraviolet absorption, antibacterial and anti-inflammatory, anti-cancer and hypolipidemic, etc. It has broad application prospects in medicine, cosmetics, papermaking and other fields. [0004] At present, the methods for producing ferulic acid mainly include plant extraction method...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N9/18C12P7/42C12R1/07
CPCC12N1/20C12N9/18C12P7/42C12Y301/01073C12N1/205C12R2001/07
Inventor 张涛江波段晓莉缪铭
Owner JIANGNAN UNIV
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