Application of ranae chensinensis seeds and extracts thereof in preparing sleep improvement products
By using frog seeds and their extracts, especially frog seed oil, to prepare drugs and health products, the problems of drug resistance and side effects of existing chemically synthesized drugs have been solved, achieving safe and effective sleep improvement.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2026-06-08
- Publication Date
- 2026-07-10
AI Technical Summary
Existing chemically synthesized drugs for improving sleep suffer from drug resistance, dependence, and side effects, while there is a lack of safe and effective sleep improvement products from natural sources.
Oils are prepared from frog seeds and their extracts, especially frog seed oil, through supercritical CO2 extraction or cold pressing, and then combined with pharmaceutically acceptable excipients to make drugs, health products, or special diets for improving sleep.
Forest frog seed extract can shorten the time to fall asleep, improve sleep efficiency, prolong sleep duration, improve the quality of deep sleep, and relieve sleep disorders caused by nervous tension. It has excellent biocompatibility and multiple auxiliary effects.
Smart Images
Figure CN122351297A_ABST
Abstract
Description
Technical Field
[0001] This invention belongs to the field of sleep health technology, specifically relating to the application of forest frog seeds and their extracts in the preparation of sleep-improving products. Background Technology
[0002] With the fast pace of life and increasing work pressure, sleep disorders have become a common health problem. According to the World Health Organization, about one-third of the global population suffers from varying degrees of sleep problems, such as difficulty falling asleep, difficulty maintaining sleep, and poor sleep quality. Chronic sleep deprivation can seriously affect daily work and life, and may also lead to other diseases. Clinically, medications used to improve sleep are mainly chemically synthesized drugs such as benzodiazepines and non-benzodiazepines. While they are relatively fast-acting, they have side effects such as drug tolerance, dependence, drowsiness the following day, and impaired cognitive function. Therefore, developing safe, effective, naturally derived sleep improvement products suitable for long-term use has become a primary goal in the health field.
[0003] Forest frog seeds and their extracts, including forest frog seed oil, are animal-derived fats extracted from forest frog seeds. These seeds and extracts are rich in various nutrients and have high nutritional value. However, research on their related efficacy is insufficient, hindering the development of high-value-added products. Current research indicates that forest frog seeds have anti-fatigue, immunity-boosting, and weight-loss effects. However, no reports have been found regarding the sleep-improving effects of forest frog seeds and their extracts. Summary of the Invention
[0004] This invention provides the application of frog seeds and their extracts in the preparation of sleep-improving products. The purpose is to provide the application of frog seed oil in improving sleep, and to provide new directions and technical support for its development in the fields of medicine, health food, and special dietary foods.
[0005] The technical solution adopted in this invention is the application of frog seeds in the preparation of drugs, health products or special diets that improve sleep.
[0006] The application of frog seed extract in the preparation of drugs, health products or special diets with sleep-improving functions, wherein the frog seed extract is frog seed oil.
[0007] The product of this invention contains an effective amount of frog seeds or frog seed oil and pharmaceutically or food-grade excipients.
[0008] The product described in this invention is a formulation made with frog seeds or frog seed oil as the active ingredient and other pharmaceutically acceptable auxiliary ingredients.
[0009] The sleep improvement methods described in this invention include: shortening the time to fall asleep, prolonging the duration of sleep, improving the quality of deep sleep, or alleviating sleep disorders caused by nervous tension.
[0010] The frog seed extract described in this invention is an oil obtained by supercritical CO2 extraction or cold pressing of frog seeds.
[0011] The conditions for supercritical CO2 extraction described in this invention are: extraction pressure 20–40 MPa, extraction temperature 35–55℃, separation pressure 5–10 MPa, separation temperature 30–45℃, CO2 flow rate 55–85 L / h, and extraction time 1–4 hours.
[0012] The cold pressing method described in this invention includes: drying frog seeds to a moisture content of ≤8%, pressing them at a temperature of ≤50℃ and a pressure of 30–60MPa, and then removing impurities by centrifugation or filtration to obtain frog seed oil.
[0013] The effective daily dose of the frog seeds described in this invention is 1g-50g.
[0014] The effective daily dose of the frog seed extract described in this invention is 0.1g - 5g.
[0015] The beneficial effects of this invention are as follows: Forest frog seeds and their extracts, as naturally derived active substances, possess excellent biocompatibility. Forest frog seeds and their extracts not only improve sleep but also have auxiliary effects such as regulating blood lipids, combating fatigue, and enhancing immunity, contributing to a comprehensive improvement in physical fitness. Animal pharmacodynamic experiments have confirmed that the forest frog seeds and their extracts of this invention can improve sleep. Specifically, this sleep-improving function encompasses one or more of the following aspects: shortening sleep onset time and alleviating sleep anxiety; improving sleep onset efficiency; prolonging sleep duration and increasing total sleep volume; reducing the number of nighttime awakenings and improving sleep continuity; improving the quality of deep sleep, increasing the proportion of deep sleep, and promoting bodily recovery. Attached Figure Description
[0016] Figure 1 This is a comparison chart of sleep durations across different treatment groups; Figure 2 This is a comparison chart of sleep delay times for the untreated group. Detailed Implementation
[0017] Example 1 After removing impurities, washing, and drying, the frog eggs were crushed and passed through a 40-mesh sieve. A supercritical CO2 extraction device was used, with the extraction pressure set at 30 MPa, extraction temperature at 45℃, separation pressure at 8 MPa, separation temperature at 38℃, CO2 flow rate at 80 L / h, and extraction time at 2.5 hours. The extract was collected and centrifuged to obtain frog egg oil, with an oil yield of 13.6%. The obtained oil was pale yellow and transparent. Example 2
[0018] After removing impurities, washing, and drying, the frog eggs were crushed and passed through a 40-mesh sieve. A supercritical CO2 extraction device was used, with the extraction pressure set at 20 MPa, extraction temperature at 35℃, separation pressure at 5 MPa, separation temperature at 30℃, CO2 flow rate at 55 L / h, and extraction time at 1 hour. The extract was collected and centrifuged to obtain frog egg oil. Example 3
[0019] After removing impurities, washing, and drying the frog eggs, they were pulverized and passed through a 40-mesh sieve. A supercritical CO2 extraction device was used, with the extraction pressure set at 40 MPa, extraction temperature at 55℃, separation pressure at 10 MPa, separation temperature at 45℃, CO2 flow rate at 85 L / h, and extraction time at 1 hour. The extract was collected and centrifuged to obtain frog egg oil. Example 4
[0020] Dried frog eggs were collected, cleaned, and dried in a 40℃ oven until the moisture content was ≤8%. The eggs were then pressed using a hydraulic oil press at 45℃ and 45MPa. The pressed liquid was collected, centrifuged at 4000 rpm for 15 minutes, and filtered to remove residue, yielding frog egg oil with a yield of 12.3%. The resulting oil was light in color and had the characteristic aroma of frog eggs. Example 5
[0021] Dried frog eggs were collected, cleaned and purified, and then dried in an oven at 40℃ until the moisture content was ≤7%. The eggs were then pressed using a hydraulic oil press at 40℃ and 30MPa. The pressed liquid was collected, centrifuged at 4000r / min for 12min, and filtered to remove residue, yielding frog egg oil. Example 6
[0022] Dried frog eggs were collected, cleaned and purified, and then dried in an oven at 40℃ until the moisture content was ≤6%. The eggs were then pressed using a hydraulic oil press at 50℃ and 60MPa. The pressed liquid was collected, centrifuged at 4000r / min for 18min, and filtered to remove residue, yielding frog egg oil.
[0023] Example 7 Thirty ICR mice (6-8 weeks old) were randomly divided into three groups: a blank control group (CG), a high-dose frog seed oil group (RH), and a low-dose frog seed oil group (RL). The CG group was administered an equal volume of saline by gavage, the RH group was administered 400 mg / kg of frog seed oil by gavage, and the RL group was administered 200 mg / kg of frog seed oil by gavage, for 28 days. After the last administration, the mice were observed to exhibit sleep patterns. The results showed that frog seed oil had no effect on sleep.
[0024] Table 1 Results of the direct sleep experiment in mice of each group (x̄±s, n=10) Group Number of people falling asleep Sleep incidence CG 0 0 RH 0 0 RL 0 0 Example 8
[0025] Similar to Example 7, 30 minutes after the last administration to the mice, each group of mice was intraperitoneally injected with sodium pentobarbital (45 mg / kg). Figure 1 The results showed that frog seed oil can prolong sleep duration. Example 9
[0026] Similar to Example 7, 30 minutes after the last administration, mice in each group were intraperitoneally injected with sodium pentobarbital (30 mg / kg, injection volume 10 mL / kg). The mice were then placed in a quiet environment, and their sleep behavior was continuously observed. The results showed that the number of animals falling asleep in the drug-treated groups increased compared to the CG group (1 mouse) (3 mice in the RH group and 2 mice in the RL group). Example 10
[0027] Similar to Example 7, 30 minutes after the last administration of the drug to the mice, each group of animals was intraperitoneally injected with sodium barbital (dose of 260 mg / kg). The criterion for sleep onset was the duration of absence of the righting reflex exceeding 1 minute. The time required from the completion of injection to the animal falling asleep was recorded. (Appendix) Figure 2 The results showed that frog seed oil can shorten the sleep latency period and promote sleep. Example 11
[0028] Soft capsules made from 300g of frog seed oil, 100g of soybean oil, 2g of vitamin E, 1g of vitamin B2, gelatin, glycerin, purified water, titanium dioxide, etc., can be used for people who have difficulty falling asleep. Example 12
[0029] Forest frog seeds are dried at low temperature and coarsely ground, then ultra-finely ground using an ultra-micro pulverizer at ≤10℃ to obtain ultra-fine forest frog seed powder with a particle size D90≤20μm. The mixture consists of 30g of forest frog seed ultra-fine powder, 15g of jujube seed powder, 10g of Poria cocos powder, 20g of erythritol, 1g of citric acid, and 4g of fruit powder. The mixture is thoroughly mixed and packaged into 5g packets. Adults are recommended to take 1-2 packets with 150mL of warm water before bedtime daily. The daily dose of forest frog seeds is approximately 3g-6g. Example 13
[0030] 25g of raw frog seed powder (passed through a 100-mesh sieve), 60g of sorbitol, 10g of microcrystalline cellulose, 1.5g of magnesium stearate, and 0.5g of peppermint flavoring are mixed evenly and compressed into tablets. Each tablet weighs 0.8g and contains 0.2g of raw frog seed powder. Adults are recommended to take 2-4 tablets daily before bedtime. Example 14
[0031] 300g of frog seed oil, 200g of modified starch, 180g of maltodextrin, 50g of sodium caseinate, and 5g of glyceryl monostearate were dissolved in an appropriate amount of purified water. The frog seed oil was slowly added under high-speed shear conditions, followed by homogenization and emulsification (40MPa, twice). After spray drying (inlet air temperature 175℃, outlet air temperature 80℃), frog seed oil microcapsule powder was obtained, with an encapsulation rate of 93.2% and an oil loading of 30.5%. 10g of microcapsule powder, 8g of erythritol, and 0.5g of citric acid were mixed and packaged into 5g packets. Each packet contains approximately 1.0g of frog seed oil. The recommended adult dosage is one packet taken before bedtime daily. Example 15
[0032] Ingredients: 200g frog seed oil, 60g gamma-aminobutyric acid (GABA), 10g lecithin, 5g beeswax, gelatin, glycerin, and purified water. Soft capsules are prepared using a soft capsule pressing device. Each capsule contains 550mg of contents (approximately 380mg of frog seed oil and 110mg of GABA). Recommended adult dosage: 1-2 capsules daily before bedtime.
Claims
1. Application of frog seeds in the preparation of drugs, health products or special diets that improve sleep.
2. The application of frog seed extract in the preparation of drugs, health products or special diets with sleep-improving functions, wherein the frog seed extract is frog seed oil.
3. The application according to claim 1 or 2, characterized in that, The product contains an effective amount of frog seeds or frog seed oil, as well as pharmaceutically or food-grade excipients.
4. The application according to claim 1 or 2, characterized in that, The product is a formulation made with frog seeds or frog seed oil as the active ingredient and other pharmacologically acceptable auxiliary ingredients.
5. The application according to claim 1 or 2, characterized in that, The improvements in sleep include: shortening the time it takes to fall asleep, prolonging the duration of sleep, improving the quality of deep sleep, or alleviating sleep disorders caused by nervous tension.
6. The application according to claim 2, characterized in that, The frog seed extract is an oil obtained by extracting frog seeds through supercritical CO2 extraction or cold pressing.
7. The application according to claim 6, characterized in that, The conditions for supercritical CO2 extraction are: extraction pressure 20–40 MPa, extraction temperature 35–55℃, separation pressure 5–10 MPa, separation temperature 30–45℃, CO2 flow rate 55–85 L / h, and extraction time 1–4 hours.
8. The application according to claim 6, characterized in that, The cold pressing method includes: drying the frog seeds to a moisture content of ≤8%, pressing them at a temperature of ≤50℃ and a pressure of 30–60 MPa, and then removing impurities by centrifugation or filtration to obtain frog seed oil.
9. The application according to claim 1, characterized in that, The effective daily dose of the frog seeds is 1g - 50g.
10. The application according to claim 2, characterized in that, The effective daily dose of the frog seed extract is 0.1g - 5g.