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Fractionation of hemicellulosic materials

A technology for hemicellulose and raw materials, applied in the field of enzyme modification of hemicellulose, can solve the problems of expensive sources, limited range of gel strength, etc., to reduce production costs, avoid alcohol precipitation steps, and maximize yield the effect of

Inactive Publication Date: 2000-10-04
EI DU PONT DE NEMOURS & CO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, lactase is unacceptable in some food sectors, expensive and limited
Furthermore, oxidases such as lactase are relatively weak oxidation promoters and the range of different gel strengths achievable with this enzyme is limited

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0175] 10 g of corn bran was cultured in 500 mL of water containing 200 units of ferulic acid esterase produced by Aspergillus niger at pH 5 and 35° C. (1 unit = 1 mmol ferulic acid released per minute). Free ferulic acid was monitored based on the absorption of the supernatant at 310nm. The release of ferulic acid from corn bran is shown in the following table, and the properties of the extracted AXF gel (2wt%, gelled with peroxidase / hydrogen peroxide) are compared.

[0176] Time (min) Relative content of free ferulic acid Gel strength

[0177] 15 15 high

[0178] 30 50 in

[0179] 60 70 low

[0180] 120 100 zero (liquid)

Embodiment 2

[0182] 100g of wheat bran was cultured in 5L of water at pH 5 and 50°C with 500 units of acetyl xylan esterase derived from barley sprouts. The extraction of AXF was continuously monitored by SE-HPLC on the centrifuged supernatant during 3 hours.

[0183] Time (min) AXF yield (%)

[0184] 15 0

[0185] 45 1

[0186] 90 3

[0187] 180 6

[0188] When peroxidase / hydrogen peroxide is used for gelation (2% w / w), all recovered AXF has high gel strength.

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PUM

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Abstract

Disclosed is a process that uses selective enzymes from microbial or plant sources to facilitate the extraction of hemicellulose. Selective removal of acetate using acetyl xylan esterase (E.C.3.1.1.6) under conditions that will not hydrolyse the feruoylate esters. This treatment renders the arabinoxylan ferulate soluble under mild aqueous conditions without the use of harsh alkalis. Arabinoxylan ferulate is extracted intact and subsequently the level of ferulic acid can be controlled to give the degree of crosslinking required by treatment of the soluble arabinoxylan ferulate with ferulic acid esterase.

Description

Invention field [0001] The present invention relates to methods for extracting and modifying various compositions obtained from plant tissues, in particular to an enzymatic modification method for hemicellulose. Specifically, the present invention relates to the enzymatic treatment and post-processing of various hemicellulose raw materials (including gelled and non-gelled hemicellulose, gelled hemicellulose, cellulose fiber, protein and phenol extract), A method of producing products and co-products (especially industrial production methods) that have a wide variety of uses in the food and pharmaceutical industries and agriculture. Background of the invention [0002] Plant tissue can be divided into the following 8 main components: [0003] 1. Cellulose [0004] 2. Hemicellulose [0005] 3. β-glucan [0006] 4. Starch [0007] 5. Protein [0008] 6. Phenolic acid [0009] 7. Lignin [0010] 8. Wax, cutin and cork resin. [0011] Below, we will briefly discuss each of the above c...

Claims

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Application Information

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IPC IPC(8): C08B30/04C08B37/00C08H1/00C08H99/00C08L5/14C12P19/04
CPCC08H1/00C08H99/00C08B37/0024C12P19/04C08B37/0045C08L5/14C08H6/00C08B30/04C08B37/0057
Inventor C·S·菲彻特
Owner EI DU PONT DE NEMOURS & CO
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