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Feruloyl esterase production strain and method for producing feruloyl esterase by using same

A ferulic acid esterase and ferulic acid technology, applied in the field of bioengineering, can solve problems such as complex operation, ferulic esterase extraction method needs to be improved, and industrial production limitations

Active Publication Date: 2012-11-28
CHAMBROAD CHEM IND RES INST CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Patent CN200810056477.6 discloses a method for extracting ferulic acid esterase, but the operation is relatively complicated. There are many problems to be solved in the research of ferulic acid esterase, mainly the currently screened ferulic acid esterase secretion method. The secretion of microorganisms still cannot meet the requirements of industrial production of ferulic acid esterase; secondly, the extraction method of ferulic acid esterase needs to be improved, and its industrial production is limited

Method used

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  • Feruloyl esterase production strain and method for producing feruloyl esterase by using same

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Experimental program
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Effect test

Embodiment 1

[0067] (1) Activation of strains: move the strains on the slant of the test tube stored on PDA medium at 4°C to room temperature (20-25°C) for activation for 4 hours;

[0068] (2) Preparation of liquid seeds: Use 10mL of sterilized distilled water to make a suspension of the activated test tube slant strains on the aseptic operating table, and wash them into the sterile seed culture under aseptic conditions. In the Erlenmeyer flask of the base, one test tube strain was inoculated into one Erlenmeyer flask, the pH value was natural, the culture temperature was 24°C, the shaker speed was 200r / min, and the culture time was 48h;

[0069] The composition of the seed medium, by weight (w / w): 2% sucrose, 0.2% yeast extract powder, 0.05% magnesium sulfate, 0.025% potassium dihydrogen phosphate, add water to 1000mL; sterilization conditions are 121°C, 0.15Mpa Sterilize for 20 minutes;

[0070] (3) Fermentation and regulation process: Inoculate liquid seeds with 10% (v / v) inoculum into...

Embodiment 2

[0074] (1) Activation of strains: move the strains on the slant of the test tube stored on PDA medium at 4°C to room temperature (20-25°C) for activation for 4 hours;

[0075] (2) Preparation of liquid seeds: Use 10mL of sterilized distilled water to make a suspension of the activated test tube slant strains on the aseptic operating table, and wash them into the sterile seed culture under aseptic conditions. In the Erlenmeyer flask of the base, one test tube strain was inoculated into one Erlenmeyer flask, the pH value was natural, the culture temperature was 24°C, the shaker speed was 200r / min, and the culture time was 48h;

[0076] The composition (w / w) of the seed medium is: by weight (w / w): 2% sucrose, 0.6% yeast extract powder, 0.05% magnesium sulfate, 0.025% potassium dihydrogen phosphate, add water to 1000mL; sterilization conditions Sterilize at 121°C, 0.15Mpa for 20min;

[0077] (3) Fermentation and regulation process: Inoculate the liquid seeds with 5% (v / v) inoculu...

Embodiment 3

[0081] (1) Activation of strains: move the strains on the slant of the test tube stored on PDA medium at 4°C to room temperature (20-25°C) for activation for 4 hours;

[0082](2) Preparation of liquid seeds: Use 10mL of sterilized distilled water to make a suspension of the activated test tube slant strains on the aseptic operating table, and wash them into the sterile seed culture under aseptic conditions. In the Erlenmeyer flask of the base, one test tube strain was inoculated into one Erlenmeyer flask, the pH value was natural, the culture temperature was 24°C, the shaker speed was 200r / min, and the culture time was 48h;

[0083] The composition of the seed medium, by weight (w / w): 2% sucrose, 0.2% yeast extract powder, 0.05% magnesium sulfate, 0.025% potassium dihydrogen phosphate, add water to 1000mL; sterilization conditions are 121°C, 0.15Mpa Sterilize for 20 minutes;

[0084] (3) Fermentation and regulation process: Inoculate the liquid seeds with 5% (v / v) inoculum in...

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Abstract

The invention belongs to the technical field of bioengineering, and provides a feruloyl esterase high-producing strain and a method for preparing feruloyl esterase through liquid fermentation by using the same. The feruloyl esterase production strain provided by the invention is a filamentous fungus; 16S rDNA identification shows that the feruloyl esterase production strain is a Trichodermaviride; the strain code is JBSH-001; and the preservation number of China General Microbiological Culture Collection Center is CGMCC NO.5612. Feruloyl esterase can be produced through liquid fermentation by using the strain. The enzyme activity of the feruloyl esterase obtained through fermentation according to the method can reach 200 mU / ml or above; and the enzyme activity of the purified feruloyl esterase can reach 50000 mU / g or above.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and provides a microbial strain with high ferulic acid esterase production, in particular to a strain of Trichoderma viridans and a method for producing ferulic acid esterase by using the bacteria. Background technique [0002] Ferulic esterase, also known as cinnamic esterase, is a subclass of carboxylate hydrolase and an extracellular enzyme that hydrolyzes methyl ferulate, oligosaccharide ferulate, and polysaccharides The ester bond in the ferulic acid ester frees the ferulic acid. Ferulic acid plays an important role in the structure of plant cell walls. It can form junctions between lignin and lignin in plant cell walls, between lignin and hemicellulose, and between hemicellulose and hemicellulose, thereby Form a skeleton structure, making the entire cell wall hard. Ferulic acid esterase can open the dense network cross-linking structure formed by molecules such as ferulic acid, p-c...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12N9/18C12R1/885
Inventor 徐泽平马韵升史庆苓杨传伦张心青王秀芝付慧君虞凤慧
Owner CHAMBROAD CHEM IND RES INST CO LTD
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