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Method for producing 4-vinyl guaiacol by fermenting bacillus circulans

A technology of bacillus circulans and guaiacol, applied in the field of bioengineering, can solve the problems of low ferulic acid decarboxylase activity, inability to meet the requirements of industrial production, and low 4-VG content, and achieve excellent fermentation performance, The effect of low production cost and wide range of sources

Inactive Publication Date: 2014-05-14
QILU UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Many microorganisms (such as yeasts, bacteria, fungi involved in wine brewing, etc.) can use the nutrients in the culture medium to ferment and produce 4-vinylguaiacol, but because the activity of ferulic acid decarboxylase in the cells of these microorganisms is relatively low Low, the content of 4-VG produced is low (generally 2.0-5.0mg / L), so it cannot meet the requirements of industrial production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] A type of Bacillus circulans ( Bacillus circulans ) The method for producing 4-vinylguaiacol by fermentation of HG12 bacterial strain, the steps are as follows:

[0034] (1) Use an inoculation loop to pick a ring of Bacillus circulans HG12 strain from the slant of the strain test tube and inoculate it into 10 mL of liquid seed medium, and culture it for 14 hours at 37°C and 120 r / min to obtain an activated liquid seed.

[0035] (2) Inoculate the liquid seeds prepared in step (1) into 200mL liquid fermentation medium at an inoculum size of 5% (v / v), culture at 40°C and 200r / min for 12h, and add xylanase , the dosage is 20.0U / g bran; continue to cultivate at 40°C and 200r / min for 12h, add ferulic acid esterase, the dosage is 10.0U / g bran; continue at 40°C and 200r / min After culturing for 72 hours, a fermentation broth containing 4-vinylguaiacol was obtained.

[0036] (3) Filter and sterilize the fermentation broth containing 4-vinylguaiacol prepared in step (2) with a...

Embodiment 2

[0041] Utilize bacillus circulans as described in Example 1 ( Bacillus circulans ) The method for fermenting and producing 4-vinylguaiacol by strain HG12, the difference is:

[0042] In step (2), the liquid seeds prepared in step (1) were inoculated into 200 mL of liquid fermentation medium at an inoculum size of 5% (v / v), and the dosage of xylanase was 10.0 U / g bran , to prepare a fermentation broth containing 4-vinylguaiacol.

[0043] The content of 4-vinylguaiacol in the fermentation broth was detected to be 1.18g / L.

[0044] Under these conditions, 0.231 g of 4-vinylguaiacol product was obtained.

Embodiment 3

[0046] Utilize bacillus circulans as described in Example 1 ( Bacillus circulans ) The method for fermenting and producing 4-vinylguaiacol by strain HG12, the difference is:

[0047] In step (2), the liquid seed prepared in step (1) was inoculated into 200 mL of liquid fermentation medium at an inoculum size of 5% (v / v), and the dosage of ferulic acid esterase was 5.0 U / g bran bark to obtain a fermentation broth containing 4-vinylguaiacol.

[0048] The content of 4-vinylguaiacol in the fermentation broth was detected to be 1.12g / L.

[0049] Under these conditions, 0.219 g of 4-vinylguaiacol product was obtained.

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Abstract

The invention belongs to the technical field of biological engineering, and particularly discloses a method for producing 4-vinyl guaiacol by fermenting bacillus circulans. The method takes the bacillus circulans as a producion strain. The method is characterized by comprising the following steps of choosing 1-2 bacillus circulans HG12 thalli from a test tube slant, inoculating to a liquid seed culture medium, and culturing to prepare an activated liquid seed; inoculating the liquid seed into a liquid fermentation culture medium according to inoculum size, and sequentially adding xylanase and feruloyl esterase, culturing to prepare fermentation liquid which contains the 4-vinyl guaiacol; carrying out filtration, extraction and standing phase splitting on the fermentation liquid to prepare extract liquid which contains the 4-vinyl guaiacol, and carrying out vacuum evaporation on the extract liquid to prepare a 4-vinyl guaiacol product. The method disclosed by the invention can realize the continuity and scale of 4-vinyl guaiacol production; the 4-vinyl guaiacol has the advantages of simplicity and convenience for production process, short production period, low production cost and great industrialized application prospect.

Description

(1) Technical field [0001] The invention belongs to the technical field of bioengineering, in particular to a method for producing 4-vinylguaiacol by fermentation of bacillus circulans. (2) Background technology [0002] 4-Vinylguaiacol (4-Vinylguaiaco1, 4-VG) is also known as 2-methoxy-4-vinylphenol, p-vinylguaiacol, and its molecular formula is C 9 h 10 o 2 , with a molecular weight of 150.18, is a colorless or light yellow oily liquid with a slightly sweet taste and a fermented aroma. 4-VG is a volatile phenolic substance that can produce special aroma or clove-like aroma. Its sensory threshold is very low, only 0.3mg / L. The natural aroma of foods such as beverages has a great influence. 4-VG is a commonly used high-grade spice, mainly used in food to increase its aroma components, and also used in tobacco, daily chemical, medicine, flavor synthesis and other industries. In the brewing industry, 4-VG is an essential aroma substance in top-fermented beer with wheat a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/22C12R1/09
Inventor 董永胜
Owner QILU UNIV OF TECH
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