84 results about "Guaianediol" patented technology

The invention relates to an application of oxygenase in catalyzing 4-ethene guaiacol to synthesize vanillin. The amino acid sequence of the oxygenase comprises a sequence as shown in SEQ ID NO.1. The invention also relates to an application of the oxygenase in catalyzing 4-ethene guaiacol to synthesize vanillin. The invention provides an application of oxygenase in catalyzing synthesis of vanillin from 4-ethene guaiacol. The 4-ethene guaiacol oxygenase is in soluble expression in host bacteria. Crude enzyme liquid expressed by the strain stably catalyzes conversion of 4-ethylene guaiacol into vanillin, the conversion rate within 2 hours is 80%, the conversion rate within 24 hours exceeds 98%, and 11.5 g / L of vanillin can be accumulatively produced within 6 hours.

The invention relates to laccase as well as a mutant and application thereof, and belongs to the technical field of biology. The amino acid sequence of the laccase PnLac1 is as shown in SEQ ID NO. 2, and the nucleotide sequence of the laccase PnLac1 is as shown in SEQ ID NO. 1. The mutant is characterized in that aspartic acid at the 166th position of laccase PnLac1 is subjected to site-specific mutagenesis, histidine at the 348th position of laccase PnLac1 is subjected to site-specific mutagenesis, methionine at the 403th position of laccase PnLac1 is subjected to site-specific mutagenesis, or leucine at the 467th position of laccase PnLac1 is subjected to site-specific mutagenesis, amino acid sequences are shown as SEQ ID NO.4, SEQ ID NO.6, SEQ ID NO.8 and SEQ ID NO.10, and nucleotide sequences are shown as SEQ ID NO.3, SEQ ID NO.5, SEQ ID NO.7 and SEQ ID NO.9. The decoloring efficiency of the laccase PnLac1 on crystal violet reaches 5h, and the degradation rate reaches 75%. When the ABTS is used as a substrate, the enzyme activities of the laccase mutants H348F and L467M are respectively improved by 1.89 times and 1.65 times, and the ABTS has the potential of degrading stubborn substances; when guaiacol is used as a substrate, the enzyme activities of the laccase mutants D166A and M403F are respectively improved by 2.07 times and 3.72 times, and the guaiacol has a relatively high application prospect in the field of lignin.

The invention provides a Ni-molybdenum oxide / Nb2O5 nano composite catalyst as well as a preparation method and application thereof, and belongs to the technical field of catalysts. The catalyst precursor is prepared by adopting an excessive impregnation method, then the Ni-molybdenum oxide / Nb2O5 nano-composite catalyst is prepared by adopting a hydrogen reduction treatment method, active components in the catalyst are uniformly loaded, and the catalyst has excellent self-hydrogen hydrothermal catalytic performance. The Ni-molybdenum oxide / Nb2O5 nano composite catalyst prepared by the invention can minimize the dissociation energy of a C-O bond, promote the breakage of the C-O bond, enable the reaction to be carried out in a forward direction, and realize high-value conversion of guaiacol to catechol under mild reaction conditions without exogenous hydrogen.

The invention belongs to the technical field of catalysts, and provides Ni-CoO / SiO2 aerogel as well as a preparation method and application thereof. The method comprises the following steps: mixing water, tetraethoxysilane, ethanol and a hydrochloric acid solution, and hydrolyzing to obtain silica sol; the preparation method comprises the following steps: mixing silica sol, a nickel salt solution, a cobalt salt solution and a hydrochloric acid solution, and sequentially carrying out ultrasonic treatment, water bath and freeze drying to obtain SiO2 aerogel containing NiO and CoO; the preparation method comprises the following steps: in a reducing atmosphere, carrying out reduction reaction on SiO2 aerogel containing NiO and CoO to obtain the Ni-CoO / SiO2 aerogel. The Ni-CoO / SiO2 aerogel can realize hydrogen production through water photolysis of CoO, Ni promotes the hydrodeoxygenation process of guaiacol, meanwhile, the greenhouse effect of the porous structure of the SiO2 aerogel provides a high-temperature environment for the reaction process, the reaction is promoted to be continuously and positively carried out, and efficient conversion from guaiacol to phenol under the mild reaction condition is realized.

The invention discloses a 4-propyl guaiacol oxidase mutant as well as a preparation method and application thereof, wild type 4-propyl guaiacol oxidase comes from Penicillium simplicissimum, an active center of the wild type 4-propyl guaiacol oxidase is modified by using a directed evolution means, and the mutant is obtained by modifying the active center of the wild type 4-propyl guaiacol oxidase. 4-propyl guaiacol oxidase mutants D170E, S426T, S50Q / D170E, D170E / L411V and D170E / L411V / S106C with relatively high activity, cis-trans selectivity and trans-isoeugenol yield are obtained, efficient synthesis from cheap 4-propyl guaiacol to high-value trans-isoeugenol is realized, and industrial synthesis and application of the trans-isoeugenol are facilitated.

The present invention discloses a bio-based benzoxazine monomer-modified epoxy resin and a preparation method thereof. The method comprises: reacting guaiacol, ethanolamine, and paraformaldehyde to obtain a bio-based benzoxazine monomer; reacting the bio-based benzoxazine monomer with phosphorus oxychloride in a solvent to obtain a benzoxazine phosphate monomer; and uniformly mixing the benzoxazine phosphate as a modifying curing agent, a bio-based diamine Priamine 1074 as a toughening curing agent, and a bisphenol A-type epoxy resin (DGEBA). The mixture is poured into a mold, and then heated and cured to obtain the bio-based benzoxazine monomer-modified epoxy resin. The bio-based benzoxazine monomer-modified epoxy resin obtained by the present invention has strong mechanical properties and thermal stability, as well as excellent adhesion, flame retardancy, low dielectric properties, and biodegradability.

The invention discloses a catalyst for preparing phenol and guaiacol through lignin depolymerization as well as a preparation method and application of the catalyst. The catalyst comprises a carrier and active components loaded on the carrier, the carrier is activated carbon, and the active components are molybdenum, cobalt and ruthenium. The catalyst is applied to catalytic depolymerization of lignin to prepare phenol and guaiacol, 100% lignin conversion rate can be achieved in an absolute ethyl alcohol solvent in a nitrogen atmosphere under the condition that no extra hydrogen source is needed, the phenol yield is larger than 10%, the guaiacol yield is larger than 25%, the depolymerization capacity and selectivity of the catalyst to lignin can be improved, and the catalyst can be applied to preparation of phenol and guaiacol. Good industrial application prospects are realized.

The invention discloses a method for preparing guaiacol monomer products by catalyzing selective hydrogenolysis of lignin through aluminum oxide loaded nickel and nickel-tin alloy. The catalyst simultaneously containing metal nickel and nickel-tin alloy active phases is prepared by adopting a urea precipitation method, organic soluble lignin is taken as a raw material, a mixture of alcohol substances and water is taken as a solvent, and the aluminum oxide loaded metal nickel and nickel-tin alloy catalyst prepared by adopting the urea precipitation method is added; and replacing air in the reaction kettle with inert gas, pressurizing to 1.0-3.0 MPa, and stirring at 210-270 DEG C for 1-6 hours to convert the organic soluble lignin into a compound mainly comprising guaiacol monomer products with high selectivity. The catalyst is simple in preparation process and low in cost, the lignin depolymerization reaction has the advantages of mild conditions, safe operation process and the like, and the obtained guaiacol product can be used for preparing chemicals such as food additives or medical intermediates.

The invention discloses a 3-hydroxyisovaleric acid and guaiacol composition and application of the 3-hydroxyisovaleric acid and guaiacol composition in preparation of a medicine for treating or preventing glucose metabolism disorder. The invention provides a strategy for treating metabolic diseases by combining 3-hydroxyisovaleric acid and guaiacol for the first time. It is found that combination of the two has a significant synergistic effect, and combination of low doses is superior to single use of high doses. Systematic animal experiments prove that the traditional Chinese medicine composition has a remarkable effect of improving blood sugar, insulin resistance and fatty liver.

The invention provides bio-based epoxy resin based on 4-propyl guaiacol as well as preparation, degradation and reprocessing methods and application of the bio-based epoxy resin, and relates to the technical field of epoxy resin. The bio-based epoxy resin based on the 4-propyl guaiacol, provided by the invention, is prepared from the following raw materials: a 4-propyl guaiacol epoxy monomer and a curing agent; the curing agent contains amino groups and / or disulfide bonds. The bio-based epoxy resin based on 4-propyl guaiacol provided by the invention has the characteristic of being capable of being reprocessed for multiple times, and can keep the integrity of a chemical structure and the stability of thermodynamic properties after being reprocessed for multiple times. The bio-based epoxy resin based on the 4-propyl guaiacol can complete repair of surface scratches under the action of small pressure, can be chemically degraded easily in an N, N-dimethylformamide-solvent system, is convenient to degrade and recycle, and reduces pollution and damage to the ecological environment.

The invention relates to the technical field of macromolecules, in particular to a bio-based non-isocyanate polyurea material and a preparation method thereof.The preparation method comprises the steps that guaiacol sequentially reacts with epoxy chloropropane and carbon dioxide to obtain guaiacol cyclic carbonate, and then the guaiacol cyclic carbonate and a composite curing agent are heated and subjected to polycondensation to prepare the bio-based non-isocyanate polyurea material. The synthesis process is simple and convenient, the used raw materials guaiacol and beta-cyclodextrin are bio-based, consumption of non-renewable resources is reduced, and the raw materials are low in price and easy to obtain; the composite curing agent is obtained by mixing amino-modified beta-cyclodextrin and tris (2-aminoethyl) amine, so that the polyurea material has good mechanical properties. The bio-based non-isocyanate polyurea material prepared by the preparation method disclosed by the invention has good mechanical properties and thermal stability while renewable resources are utilized, and has a wide application prospect compared with the prior art.

The application discloses 4-propyl guaiacol oxidase mutants, a preparation method and application thereof, wherein wild-type 4-propyl guaiacol oxidase is from Penicillium simplicissimum Penicillium simplicissimum ​ The active center of the wild-type 4-propyl guaiacol oxidase is reformed by using a directed evolution method, and 4-propyl guaiacol oxidase mutants D170E, S426T, S50Q / D170E, D170E / L411V and D170E / L411V / S106C with high activity, cis-trans selectivity and trans-isoeugenol yield are obtained, efficient synthesis of high-value trans-isoeugenol from cheap 4-propyl guaiacol is realized, and the industrialized synthesis and application of the trans-isoeugenol are facilitated.

The invention discloses application of guaiacol in treatment of rheumatoid arthritis, and particularly relates to application of guaiacol in preparation of drugs for treating rheumatoid arthritis. Cell experiments prove that guaiacol can effectively down-regulate expression of proinflammatory factors in macrophages and up-regulate expression of anti-inflammatory factors, and has remarkable anti-inflammatory activity; animal experiments show that the guaiacol effectively relieves rheumatoid arthritis model mouse diseases, and the specific manifestation is that the guaiacol obviously relieves the mouse claw swelling degree, reduces the arthritis disease score, obviously improves joint inflammatory cell infiltration, repairs cartilage injury, inhibits osteoclast generation and reduces the number of joint apoptotic cells; meanwhile, the level of proinflammatory factors in serum of mice can be effectively reduced, and the level of proinflammatory repair factors can be up-regulated. The invention proves that guaiacol has a definite curative effect on rheumatoid arthritis, good biological safety and no obvious side effect, provides a new effective active component for the treatment of rheumatoid arthritis, and has a good prospect of being developed into the medicine for treating rheumatoid arthritis.

The invention discloses a copper molybdate catalyst, a preparation method, application and a method for producing 4-propyl phenol. A preparation method of a copper molybdate catalyst for catalyzing demethoxylation of 4-propyl guaiacol comprises the following steps: mixing water-soluble molybdate, water-soluble copper salt and water to obtain a metal aqueous solution; and concentrating, drying and calcining the metal aqueous solution to obtain the copper molybdate catalyst. The preparation method is simpler to operate and more environmentally friendly, the prepared copper molybdate catalyst can be used for catalyzing 4-propyl guaiacol to remove methoxy so as to produce 4-propyl phenol, and the catalytic performance and stability are excellent.

Use of guaijaverin in preparation of an eye protection product is provided, belonging to the technical field of biomedicine. Use of guaijaverin in preparation of an eye protection product is provided. In Examples, a human retinal primary Müller cell optical damage model, a zebrafish eye damage model by blue light, and a mouse retinal optical damage model are constructed in vitro separately. Based on an eye-protection effect of the guaijaverin, further development may be conducted on drugs for treating retinal optical damage-related diseases and functional products for alleviating visual fatigue.

The present invention relates to lignin comprising beta-O-4'units bound in position to one or more compounds of formula (I), such as phenol, catechol, resorcinol, hydroquinone, pyrogallol and / or guaiacol, a process for its preparation and its use. And lignin fragment 1.

The invention relates to application of guaiacol or a derivative thereof in preparation of a medicine for preventing or treating metabolic syndrome, and belongs to the technical field of medicines. The guaiacol and the derivative thereof provided by the invention have the advantages that by regulating a fat metabolism pathway, the insulin resistance condition is obviously improved, the inflammatory response and oxidative stress injury are relieved, and the prevention and treatment on the metabolism-related fatty liver disease (MASLD), hyperlipidemia, hypercholesteremia, obesity and diabetes mellitus are realized through the synergistic effect of multiple dimensions.

The invention discloses a diatom-loaded amorphous cerium oxide electrocatalyst and a preparation method and application thereof, and relates to the technical field of catalyst material synthesis and biomass value materials.The preparation method comprises the steps that amorphous cerium oxide is dispersed in absolute ethyl alcohol, then metal salt is added for ultrasonic treatment, and a precursor is obtained; and after the treatment is completed, obtaining a precipitate, and calcining the precipitate in an H2 / Ar atmosphere at 500-800 DEG C for 5-10 hours to obtain the diatom-loaded amorphous cerium oxide electrocatalyst. The electrocatalyst prepared by the invention has high atom loading capacity, high selectivity and multiple active sites, and shows excellent electrocatalytic performance when being applied to electrochemical catalysis of guaiacol hydrodemethylation to produce catechol.

The application relates to application of guaiacol or a derivative thereof in preparation of a medicine for preventing or treating metabolic syndrome, and belongs to the technical field of medicines. The guaiacol and the derivative thereof provided by the application can significantly improve insulin resistance conditions by regulating a fat metabolism channel, and can simultaneously reduce inflammatory reaction and oxidative stress damage, and can realize prevention and treatment of metabolic associated steatohepatitis (MASLD), hyperlipidemia, hypercholesterolemia, obesity and diabetes through synergistic action in multiple dimensions.

This invention discloses a highly selective synthesis method for isovlandrin, comprising the following steps: Step S1, reacting guaiacol with a benzylating agent in the presence of an alkaline catalyst and an organic solvent to generate o-benzyloxyanisole; Step S2, reacting the o-benzyloxyanisole with a Vilsmeier-Haack reagent for formylation to obtain 3-benzyloxy-4-methoxybenzaldehyde; Step S3, mixing 3-benzyloxy-4-methoxybenzaldehyde with a 65wt%-70wt% aqueous sulfuric acid solution to perform an acid debenzylation reaction to obtain crude isovlandrin; Compared with the prior art, the yield of isovlandrin can reach up to 70% or more, and the selectivity is greatly improved.

A process for producing rotundone from α-guaiene, in particular by oxidation of the C(3) position, wherein the α-guaiene is produced from a precursor by a sesquiterpene synthase. The sesquiterpene synthase is produced in a microorganism.

The invention belongs to the field of bio-based epoxy resin, and discloses a preparation method of bio-based novolac epoxy resin. The preparation method comprises the following steps: by taking guaiacol from a biological source and a formaldehyde solution as raw materials, reacting under an acidic condition to prepare an epoxy resin curing agent guaiacol phenolic resin with a plurality of phenolic hydroxyl groups, and reacting with epichlorohydrin to prepare the epoxy resin monomer. The invention provides a high bio-based content epoxy resin prepared from guaiacol. The bio-based epoxy resin is excellent in performance, has the advantages of being green, high in bio-based content, high in curing activity, good in fluidity and the like, can be used as an epoxy resin monomer used in an epoxy molding compound, and plays a certain role in improving the molding process of the epoxy molding compound.

The application discloses a method for in-situ aqueous phase catalytic conversion of guaiacol and lignin into phenolic compounds, and belongs to the technical field of catalytic conversion for preparing phenolic compounds. The method adopts guaiacol or lignin to selectively generate phenol through HDO under the action of a Ni / MgO catalyst. In the absence of external hydrogen and under environmentally-friendly conditions, a one-pot reaction of the Ni / MgO catalyst can obtain a phenolic (phenol and methyl phenol) yield of up to 12.1 wt% from industrial lignin. The application opens up a non-noble metal catalytic strategy, realizes the conversion from renewable lignin resources to phenolic compounds in the absence of external hydrogen, and is consistent with the concept of green chemistry and sustainable development.

The present invention relates to a process for the preparation of catechol and use of said catechol directly for the preparation of guaiacol, guaethol, vanillin and ethylvanillin.

The invention belongs to the technical field of guaiacol hydrogenation catalysts, and particularly relates to a mesoporous SiO2 coated Mo2C-Co core-shell nanoparticle catalyst as well as a preparation method and application thereof. According to the invention, Mo2C-Co metal nanoparticles and mesoporous silica coated outside the Mo2C-Co metal nanoparticles are adopted to form a catalyst, and high activity, high stability and unprecedented high cyclohexanol selectivity in a hydrodeoxygenation reaction of guaiacol are realized through a unique core-shell structure (Mo2C-Co core + mesoporous SiO2 shell) and a bimetallic synergistic effect (Mo2C and Co) of the catalyst. The invention not only provides a high-efficiency catalyst for solving the key problem of product selectivity control in biomass conversion, but also opens up a more attractive green path for producing a high value-added chemical cyclohexanol, and has remarkable technical advantages and application prospects.

The invention discloses a catalyst, a preparation method and application thereof, and a production method of 4-propyl phenol. The invention relates to a catalyst for a demethoxylation reaction of 4-propyl guaiacol, which is a Cu-Mo-SiO2 catalyst and is formed by a molybdenum-containing inorganic substance, a copper-containing inorganic substance and a silicon-containing organic substance, based on the total mass of the catalyst, the mass fraction of the Mo element is 14.8-16.3 wt%, and the mass fraction of the Cu element is 0.9-9.8 wt%. The catalyst can be used for catalyzing 4-propyl guaiacol to demethoxylate in a fixed bed reactor so as to generate 4-propyl phenol.

The invention discloses a CoOx / ZSM (Zeolite Socony Mobil) catalyst as well as a preparation method and application thereof, and the preparation method comprises the following steps: loading cobalt on a ZSM-5 molecular sieve by a wet impregnation method, and drying to obtain a precursor; calcining the precursor in air, and calcining and reducing in hydrogen to obtain the CoOx / ZSM catalyst. The method comprises the following steps: mixing biomass, a CoOx / ZSM catalyst and a methanol / water mixed solvent, reacting in an H2 atmosphere, extracting a liquid product to obtain 4-n-propyl guaiacol, and separating the CoOx / ZSM catalyst in solid residues to obtain biomass residues. The prepared catalyst does not need precious metal, the reaction condition is mild, the high efficiency and selectivity of the catalyst can be kept in multiple cycles, the ratio of Co to CoO is achieved by regulating and controlling the hydrogen reduction temperature, the hydrogen overflow effect is accurately controlled, and the contradiction among high efficiency, selectivity, mild conditions and cost of a traditional RCF technology is solved.

The invention discloses sweet plum essence and a preparation method thereof. The sweet plum essence is prepared from the following components in parts by mass: 0.01 to 0.05 part of eugenol, 0.02 to 0.10 part of alpha damascenone, 0.05 to 0.20 part of beta damascenone, 0.20 to 1.00 part of guaiacol, 0.20 to 1.00 part of myricetin, 0.30 to 1.50 part of ethyl maltol, 5 to 20 parts of dark plum tincture, 5 to 20 parts of jujube tincture and 50 to 90 parts of propylene glycol. The preparation method comprises the following steps: carrying out clarification pretreatment on dark plum tincture and jujube tincture, dissolving ethyl maltol, eugenol, guaiacol, alpha-damascenone and beta-damascenone in propylene glycol at 35-45 DEG C, cooling to not higher than 30 DEG C, adding myricetin, uniformly mixing, adding a clarified tincture, curing for 12-72 hours, and carrying out 0.22-0.45 mu m microfiltration to obtain a finished product. The obtained essence is clear and stable, good in batch consistency, harmonious in sweet plum style level and lasting in aftertaste, and is suitable for aroma enhancement of foods or beverages.

To provide an animal repellent and an animal repelling method that exhibit a rapid repelling effect against animals and a high sustained repelling effect without olfactory habituation.SOLUTION: The animal repellent contains phenols, such as guaiacol, 3-methoxyphenol, 4-ethylguaiacol, phenol, creosol, o-cresol, m-cresol, and p-cresol as active ingredients.SELECTED DRAWING: Figure 1

A core-shell structured catalyst comprising: a metal nanoparticle, a non-acidic, spherically shaped inner core, and an acid, spherically shaped outer shell, wherein the metal nanoparticle is supported on the spherically shaped inner core. Also disclosed is a method for preparing the core-shell structured catalyst and use thereof for the hydrodeoxygenation of guaiacol to produce an oxygen-free, polycyclic, high-density fuel precursor.