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Method for preparing low-viscosity Semen plantaginis polysaccharides through enzyme technology

A technology for the preparation of psyllium polysaccharide and enzymatic method, which is applied in the field of natural product preparation

Inactive Publication Date: 2015-11-11
NANCHANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the high viscosity of psyllium polysaccharide, it encounters many inconveniences in the process of extraction, purification and subsequent production. Reducing its viscosity will greatly facilitate its basic research and product development and application.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Soak the psyllium in 80% ethanol for 24 hours, filter and collect the psyllium, and place it in a drying oven to adjust the temperature at 50 o C, drying and removing impurities. Take by weighing 100g of the above-mentioned Semen Plantaginis polysaccharide, adopt boiling water method to extract Semen Plantaginis polysaccharide, extraction temperature is 100 o C, the mass-to-volume ratio of solid to liquid (g / mL) is 1:10, and the extraction is performed twice, and the extraction time is 3 hours each time. The extract was centrifuged at a speed of 4800r / min for 10min. After the supernatant was concentrated in vacuum, ethanol with a mass fraction of 95% was added for precipitation. The final mass fraction of ethanol in the system was 80%. Refrigerate overnight at 4°C, and then The precipitate was collected by centrifugation at a speed of 4800r / min and a time of 10min. The precipitate was washed twice with acetone, anhydrous ether and absolute ethanol in sequence, and the...

Embodiment 2

[0017] Soak the psyllium in 80% ethanol for 24 hours, filter and collect the psyllium, and place it in a drying oven to adjust the temperature at 50 o C, drying and removing impurities. Take by weighing 100g of the above-mentioned Semen Plantaginis polysaccharide, adopt boiling water method to extract Semen Plantaginis polysaccharide, extraction temperature is 100 o C, the mass-to-volume ratio of solid to liquid (g / mL) is 1:10, and the extraction is performed twice, and the extraction time is 3 hours each time. The extract was centrifuged at a speed of 4800r / min for 10min. After the supernatant was concentrated in vacuum, ethanol with a mass fraction of 95% was added for precipitation. The final mass fraction of ethanol in the system was 80%. Refrigerate overnight at 4°C, and then The precipitate was collected by centrifugation at a speed of 4800r / min and a time of 10min. The precipitate was washed twice with acetone, anhydrous ether and absolute ethanol in sequence, and the...

Embodiment 3

[0019] Soak the psyllium in 80% ethanol for 24 hours, filter and collect the psyllium, and place it in a drying oven to adjust the temperature at 50 o C, drying and removing impurities. Take by weighing 100g of the above-mentioned Semen Plantaginis polysaccharide, adopt boiling water method to extract Semen Plantaginis polysaccharide, extraction temperature is 100 o C, the mass-to-volume ratio of solid to liquid (g / mL) is 1:10, and the extraction is performed twice, and the extraction time is 3 hours each time. The extract was centrifuged at a speed of 4800r / min for 10min. After the supernatant was concentrated in vacuum, ethanol with a mass fraction of 95% was added for precipitation. The final mass fraction of ethanol in the system was 80%. Refrigerate overnight at 4°C, and then The precipitate was collected by centrifugation at a speed of 4800r / min and a time of 10min. The precipitate was washed twice with acetone, anhydrous ether and absolute ethanol in sequence, and the...

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Abstract

A method for preparing low-viscosity Semen plantaginis polysaccharides through an enzyme technology comprises the following steps: 1, drying Semen plantaginis, and removing impurities; 2, extracting Semen plantaginis polysaccharides through adopting a boiling water technology; 3, purifying the Semen plantaginis polysaccharides; 4, weighing a certain amount of the purified Semen plantaginis polysaccharides, dissolving the polysaccharides according to a mass / volume ratio (mg / mL) of the polysaccharides to an aqueous solution of 1:1-5:1, adding feruloyl esterase according to an enzyme activity / mass ratio (U / mg) of feruloyl esterase to the polysaccharides of 0.1-0.5, and allowing the feruloyl esterase to act for 2-24h; reacting the obtained polysaccharide and feruloyl esterase mixed system in 40DEG C water bath; and dialyzing a solution obtained after the above reaction, concentrating after dialysis ends, and freeze-drying to obtain the low-viscosity Semen plantaginis polysaccharides. The low-viscosity Semen plantaginis polysaccharides are prepared under mild conditions; and the basic structure characteristics of the polysaccharides have no obvious change before and after enzyme technology treatment.

Description

technical field [0001] The invention belongs to the field of natural product preparation. Background technique [0002] Psyllium psyllium Plantago asiatica L. or flat car front Plantagoderpressa The dry mature seeds of Willd. are obtained by harvesting the ears when the seeds are ripe in summer and autumn, rubbing out the seeds and removing impurities. It is now listed as a health food item by my country's Food and Drug Administration. Plantago seed coat is rich in viscous polysaccharides, which have the functions of laxative, preventing the diffusion of harmful substances, and beneficial to intestinal health, so it is widely used at home and abroad. [0003] Psyllium polysaccharide is a polysaccharide with high viscosity, rich in arabinoxylan, and can form a weak gel under certain conditions. However, due to the high viscosity of psyllium polysaccharide, it encounters many inconveniences in the process of extraction, purification and subsequent production. Reducing its ...

Claims

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Application Information

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IPC IPC(8): C08B37/00
Inventor 聂少平殷军艺蔡思佳施晓丹
Owner NANCHANG UNIV
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