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A kind of method for producing ferulic acid esterase by fermentation of Aspergillus fumigatus

A technology of ferulic acid esterase and Aspergillus fumigatus, which is applied in the field of bioengineering and microbial fermentation, can solve the problems of limiting the application of ferulic acid esterase and insufficient tolerance, and achieve fast and convenient enzyme activity determination with strong tolerance , the effect of mild conditions

Inactive Publication Date: 2011-12-21
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there is a great demand for ferulic acid esterase in industry, and the ferulic acid esterase used in industry is mainly produced by Aspergillus niger, because the ferulic acid esterase of Aspergillus niger is resistant to high temperature, acid or alkali and other conditions Insufficient performance limits the industrial application of ferulic acid esterase to a certain extent

Method used

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  • A kind of method for producing ferulic acid esterase by fermentation of Aspergillus fumigatus
  • A kind of method for producing ferulic acid esterase by fermentation of Aspergillus fumigatus

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Effect test

Embodiment 1

[0020] Ferulic acid esterase comparison produced by microbial fermentation under the induction of embodiment 1 corn flour

[0021] (1) Preparation of culture medium: take KH 2 PO 4 3g, Na 2 HPO 4 ·7H 2 O 6g, NaCl 0.1g, yeast powder 8g, MgSO 4 ·7H 2 O 0.2g, CaCl 2 0.05g, 20g of corn flour crushed to 40 mesh, added to 1000mL of water, heated and mixed to obtain a culture solution; the culture solution was divided into 250mL Erlenmeyer flasks, and each Erlenmeyer flask contained 30mL of culture solution.

[0022] (2) Activation of the strain: inoculate the Aspergillus fumigatus preserved at 4°C on the potato agar slant medium, and activate and cultivate it at 28°C for 3 days; scrape the activated Aspergillus fumigatus into sterile water with an inoculation needle, shake A suspension of Aspergillus fumigatus spores was obtained, and the concentration of Aspergillus fumigatus spores in the suspension of Aspergillus fumigatus was 1×10 6 individual / mL. The above-mentioned ...

Embodiment 2-5

[0033] The influence of different fermentation time under the induction of embodiment 2-5 corn flour on ferulic acid esterase produced by Aspergillus fumigatus

[0034] (1) Preparation of culture medium: take KH 2 PO 4 3g, Na 2 HPO 4 ·7H 2 O 6g, NaCl 0.1g, yeast powder 8g, MgSO 4 ·7H 2 O 0.2g, CaCl 2 0.05g, 20g of corn flour crushed to 60 mesh, added to 1000mL of water, heated and mixed to obtain a culture solution; the culture solution was divided into 250mL Erlenmeyer flasks, and each Erlenmeyer flask contained 30mL of culture solution.

[0035] (2) Activation of the bacterial strain: inoculate the Aspergillus fumigatus (source as above) preserved at 4°C on the potato agar slant medium, activate and cultivate it for 3 days at 28°C; scrape the activated Aspergillus fumigatus into the sterile Aspergillus fumigatus spore suspension was prepared by shaking in bacterial water, and the concentration of Aspergillus fumigatus spores in the suspension was 1×10 6 individual / ...

Embodiment 6-9

[0042] Effects of different fermentation times on the production of ferulic acid esterase by Aspergillus fumigatus under the induction of embodiment 6-9 bran

[0043] (1) Preparation of culture medium: take KH 2 PO 4 3g, Na 2 HPO 4 ·7H 2 O 6g, NaCl 0.1g, yeast powder 8g, MgSO 4 ·7H 2 O 0.2g, CaCl 2 0.05g, 20g of bran crushed to 60 mesh, added to 1000mL of water, heated and mixed to obtain a culture solution; the culture solution was divided into 250mL Erlenmeyer flasks, and each Erlenmeyer flask contained 30mL of culture solution.

[0044] (2) Activation of the bacterial strain: inoculate the Aspergillus fumigatus (source as above) preserved at 4°C on the potato agar slant medium, activate and cultivate it for 3 days at 28°C; scrape the activated Aspergillus fumigatus into the sterile Aspergillus fumigatus spore suspension was prepared by shaking in bacterial water, and the concentration of Aspergillus fumigatus spores in the suspension was 1×10 6 individual / mL.

[...

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Abstract

The invention discloses a method for producing feruloyl esterase by the fermentation of aspergillus fumigatus. The activated aspergillus fumigatus with the preservation number of CGMCC No. 3.5835 is inoculated into culture medium, and is fermented under the temperature of 25 DEG C to 30 DEG C for two to six days, wherein the culture medium comprises corn starch or bran and basal liquid culture medium. The method comprises the following steps: the corn starch is adopted as the main material of the fermentation medium, the feruloyl esterase is produced after the aspergillus fumigatus is fermented in the liquid for four to five days, and the enzyme activity stably reaches more than 98U / L. The material source is wide, and the growth and multiplication of bacterial strains are fast; the technique is simple, and conditions are mild, and are easy to control; and moreover, the enzyme activity of the produced feruloyl esterase is high and highly stable, the measurement of enzyme activity is convenient, and the invention provides a new idea for the industrialized production of the feruloyl esterase.

Description

technical field [0001] The invention belongs to the fields of bioengineering and microbial fermentation, and in particular relates to a method for producing ferulic acid esterase by fermentation of Aspergillus fumigatus. Background technique [0002] Ferulic acid (4-hydroxy-3-methoxycinnamic acid) exists in a large amount in plants and food raw materials. Studies have found that ferulic acid plays an important role in antioxidant, antithrombotic, blood lipid lowering, coronary heart disease prevention, and antibacterial. Anti-inflammation, anti-mutation and anti-cancer and other aspects have shown strong activity. In plant cell wall lignin, phenolic acids such as ferulic acid, p-coumaric acid, and dimerized ferulic acid form a dense network cross-linked structure with hemicellulose branches in the form of ester bonds, which restricts the space of animals or Efficient degradation of plant cell walls by microorganisms. In addition to cellulase and β-glucosidase participating...

Claims

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Application Information

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IPC IPC(8): C12N9/18C12R1/68
Inventor 陈启和方若思何国庆傅明亮董亚晨冯宇
Owner ZHEJIANG UNIV
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